Search results for "real-time"

Quantification of denitrifiers by real-time PCR

2007

Comparative analysis of PR gene expression in tomato inoculated with virulent Fusarium oxysporum f. sp. lycopersici and the biocontrol strain F. oxys…

2008

Non-pathogenic strains of Fusarium oxysporum have the capacity to protect plants against wilt induced by pathogenic strains of F. oxysporum. Among the mechanisms involved in this protection, induced systemic resistance has been demonstrated by using a split-root method. The aim of this study was to follow the accumulation kinetics of transcripts encoding 5 PR proteins in roots and leaves of tomato inoculated with a pathogenic or a protective strain in comparison to non-inoculated control. Moreover quantification of transcripts was assessed by real-time RT-PCR in cell cultures inoculated with the pathogenic or the protective strain. Results showed a lower expression of PR encoding genes in t…

Role of nitric oxide synthase (NOS) of Klebsormidium nitens: Identification and characterization of partners

2021

National audience; Nitric oxide (NO) is an important cellular signaling molecule across kingdoms. During bioticor abiotic stresses, NO burst is detected in both plants and mammals although no sequenceencoding the well described mammalian NO synthase (NOS) is highlighted in plants. Comparedto terrestrial plants, some algae present transcripts encoding the NOS-like enzyme. Amongthem, Klebsormidium nitens the model alga to study the early transition step from aquatic algaeto land plants is found. As mechanisms governing NO synthesis and signaling in green lineageremain unclear, the study of NOS from K. nitens (KnNOS) through (i) the identification ofregulator proteins, (ii) the identification …

A portable system for multiple parameters monitoring: towards assessment of health conditions and stress level in the automotive field

2019

In this work, an electronic portable combo system able to synchronously acquire multiple signals, e.g. electrocardiographic (ECG), photoplethysmographic (PPG) and breathing waveforms, is presented. The realized system is also capable of showing in real time some physiological parameters which can be used for assessing health/stress status of the volunteer, such as heart rate and breathing frequency and their trends over time. Thanks to the use of non-invasive PPG probes, of batteries as power supply, and to the possibility to add in the future additional sensors to acquire other signals, the system could also be employed inside vehicles for assessing the status of the driver. Finally, the r…

Anesthesia for Euthanasia Influences mRNA Expression in Healthy Mice and after Traumatic Brain Injury

2014

Tissue sampling for gene expression analysis is usually performed under general anesthesia. Anesthetics are known to modulate hemodynamics, receptor-mediated signaling cascades, and outcome parameters. The present study determined the influence of anesthetic paradigms typically used for euthanization and tissue sampling on cerebral mRNA expression in mice. Naïve mice and animals with acute traumatic brain injury induced by controlled cortical impact (CCI) were randomized to the following euthanasia protocols (n=10-11/group): no anesthesia (NA), 1 min of 4 vol% isoflurane in room air (ISO), 3 min of a combination of 5 mg/kg midazolam, 0.05 mg/kg fentanyl, and 0.5 mg/kg medetomidine intraperi…

Embedded Real-Time Surveillance Using Multimodal Mean Background Modeling

2008

Automated video surveillance applications require accurate separation of foreground and background image content. Cost-sensitive embedded platforms place real-time performance and efficiency demands on techniques to accomplish this task. In this chapter, we evaluate pixel-level foreground extraction techniques for a low-cost integrated surveillance system. We introduce a new adaptive background modeling technique, multimodal mean (MM), which balances accuracy, performance, and efficiency to meet embedded system requirements. Our evaluation compares several pixel-level foreground extraction techniques in terms of their computation and storage requirements, and functional accuracy for three r…

Application Layer FEC for Mobile TV Delivery in IP Datacast Over DVB-H Systems

2009

In this paper we investigate the potential gain that can be obtained in DVB-H using application layer forward error correction (AL-FEC) to perform a multi-burst protection of the transmission for improving the reception of streaming services for mobile terminals. Compared to the conventional approach with link layer multi protocol encapsulation FEC (MPE-FEC), this technique allows to increase the robustness of the DVB-H transmission not only as a function of the capacity devoted for error repair (FEC overhead), but also as a function of the number of bursts jointly encoded. The main drawback of this approach is an increase of the network latency, that can be translated into a larger service…

How to Use the DHT22 Sensor for Measuring Temperature and Humidity with the Arduino Board

2016

Abstract The objective of this paper is to achieve a functional system in terms of hardware and software, to measure temperature and humidity. Also, this system will allow to monitoring the time. In this, we use an Arduino board with interfacing a sensor placed in local environment to measure temperature and humidity. The paper aims to achieve the following goals: - achieving a functional system in terms of hardware and software that allows measuring and monitoring temperature, humidity and the time; - using a development board for the communication with the sensor and clock; - implementation a program that allows requirements.

Improving the multiple errors detection coverage in distributed embedded systems

2004

Currently, a lot of critical applications in automobile and aircraft avionics are built on fault-tolerant real-time distributed embedded systems. Fault injection techniques have been used extensively in the experimental validation of these systems and it is a challenge to adapt them to the demands of new technologies. This paper deals with the effect of physical faults at pin level on the Communication Network Interface in a prototype based on time-triggered architecture. Due to the essential necessity of observing system behavior during injection experiments, a suitable monitor for distributed embedded systems is proposed. The monitor is used to detect failures in the value domain that cou…

DTMC modeling for performance evaluation of DW-MAC in wireless sensor networks

2016

Synchronized duty cycling (DC) aligns sensor nodes to wake up at the same time in order to reduce idle listening for medium access control (MAC) in wireless sensor networks (WSNs). Demand wakeup MAC (DW-MAC) is a popular synchronous DC MAC protocol which allows nodes to compete and transmit multiple packets in one operational cycle. This multiple packet transmission (MPT) feature makes DW-MAC more energy efficient when comparing with other existing single time competition based protocols such as sensor MAC (S-MAC). In the literature, no analytical model exists to evaluate the performance of DW-MAC. In this paper, we develop two associated discrete time Markov chain (DTMC) models and incorpo…