Search results for "regeneration"
showing 10 items of 889 documents
PALERMO SICILIAN GARDEN
2019
Palermo, città-giardino fin dalle sue origini, ha fatto del verde una delle sue espressioni più manifeste. La città conserva come in un scrigno le testimonianze più significative della storia del verde che occupa un ruolo determinante dal punto di vista ambientale, storico e paesaggistico. Agli splendidi parchi della Conca d'Oro che un tempo abbracciavano la città, fanno eco i meravigliosi giardini privati nascosti all’interno di palazzi nobiliari, chiese e monasteri e i parchi e le ville pubbliche, alcuni dei quali trasformati oggi in spazi di verde attrezzato e orti urbani. Il contributo espone gli esiti di uno studio condotto presso il Dipartimento di Architettura dell'Università di Pale…
Lights on the City
2017
At the beginning it was Chandlers Street. If “street names are like the title of chapters of the history book of a city “(cit. Ferdinand Gregorovius), Chandlers Street owns at least two of them. The first chapter is very interesting because it tells the story of the distribution throughout the city of Palermo of the associations of Arts and Crafts that, since the Fifth Century, have resettled in strategic areas of the transportation network, concurring to the definition of urban toponymy: artisans expert in candle manufacturing opened their shops in Chandlers Street. The second chapter is perhaps even more interesting because it caused a real revolution, switching on a new light in the dark…
Multinucleated giant cells in the implant bed of bone substitutes are foreign body giant cells-New insights into the material-mediated healing process
2017
In addition to macrophages, multinucleated giant cells (MNGCs) are involved in the tissue reaction to a variety of biomaterials. Especially in the case of bone substitute materials it has been assumed that the MNGCs are osteoclasts, based on the chemical and physical similarity of many materials to the calcified matrix and the bony environment in which they are used. However, many studies indicate that these cells belong to the cell line of the foreign body giant cells (FBGCs), which are of "inflammatory origin", although they have been shown to possess both a pro- and also anti-inflammatory phenotype. Moreover, no information is available about their role in the tissue reaction to bone sub…
Superstrade urbane. Dall’alta velocità alle trasformazioni contemporanee
2013
This article focuses on urban freeway deconstruction processes that recognize this infrastructure as a resource to be reclaimed for urban regeneration. Since they first appeared, freeways have been more than simple carriers of traffic flow. Following the suggestions provided by Le Corbusier, Drexler and Rudofsky in their “ROADS” exhibition, Lawrence Halprin, Reyner Banham, particular attention is paid to the shift towards a larger set of aesthetic assumptions applied to highways. Fifty years after the iconic evocation of Autopia, urban freeways no longer embody the modern value of speed: they must increasingly deal with ecological challenges and re-cycling processes. The nodes where infrast…
Epigenetic modifiers are necessary but not sufficient for reprogramming non-myelinating cells into myelin gene-expressing cells.
2010
Background Modifications on specific histone residues and DNA methylation play an essential role in lineage choice and cellular reprogramming. We have previously shown that histone modifications or combinatorial codes of transcription factors (TFs) are critical for the differentiation of multipotential progenitors into myelinating oligodendrocytes. In this study we asked whether combining global manipulation of DNA methylation and histone acetylation together with the expression of oligodendrocyte- specific TFs, was sufficient to switch the identity of fibroblasts into myelin gene-expressing cells. Methodology/Principal Findings Transfection of six oligodendrocyte-specific TFs (Olig1, Olig2…
Comparative analysis of zebrafish nos2a and nos2b genes
2008
Abstract Nitric oxide synthase (NOS) produces nitric oxide (NO) from arginine. Three NOS isoforms have been identified in mammals, namely a neuronal (NOS1), an inducible (NOS2) and an endothelial (NOS3) enzyme. In zebrafish genome, one nos1 gene and two nos2 genes (nos2a and nos2b) were observed. We cloned zebrafish nos2a cDNA and compared nos2a and nos2b sequences, expression and inducibility. When analyzed by reverse transcription-PCR, the expression of nos2a remained very low during initial development, then increased at 96 hpf, while nos2b was expressed from 6 hpf and subsequently remained stable. Expression of nos2a is detected in the head, eye and gut regions by WISH experiments perfo…
Introduction to stem cell biology in vitro. Threshold to the future.
1999
Abstract: Transplantable hematopoietic cells with multilineage reconstituting ability can be quantitated in suspensions of human or murine cells using similar assay procedures. The incorporation into these assays of stringently defined functional endpoints ensures a high degree of specificity for the cells detected. Application of these assays to stem cell-containing suspensions after they have been stimulated for several days with defined cytokines in vitro, or by a mixture of defined and/or undefined factors in vivo, has shown that net amplifications in these populations can be obtained under both circumstances. Such studies have allowed cytokine conditions that support stem cell self-ren…
P08 Analysis of Nrf2-downstream targets after fumarate treatment in dorsal root ganglia—an anti-inflammatory therapy in neurodegenerative disease?!
2012
Background Dimethylfumarate (DMF) is a new disease modifying therapy. Several studies have shown convincing data after DMF therapy in both autoimmune inflammatory diseases and neurodegenerative disorders like Huntington9s disease (HD). DMF exerts neuroprotective effects via induction of the nuclear factor E2-related factor 2 (Nrf2) and detoxification pathways. Although the exact mechanisms that lead to neurodegeneration are not fully understood the contribution of oxidative stress inducing neurodegeneration is assumed. Aims To analyse the effects of DMF on axonal growth and regeneration and to describe the influence of DMF on the Nrf2-pathway. Methods/techniques We thus investigated the eff…
Conditional transgenic mouse models: from the basics to genome-wide sets of knockouts and current studies of tissue regeneration
2008
Many mouse models are currently available, providing avenues to elucidate gene function and to recapitulate specific pathological conditions. To a large extent, successful translation of clinical evidence or analytical data into appropriate mouse models is possible through progress in transgenic or gene-targeting technology. Beginning with a review of standard mouse transgenics and conventional gene targeting, this article will move on to discussing the basics of conditional gene expression: the tetracycline (tet)-off and tet-on systems based on the transactivators tet-controlled transactivator (Tta) and reverse tet-on transactivator (rtTA) that allow downregulation or induction of gene exp…
Regulation of cytokinesis and its clinical significance.
2015
Dysregulation of the cell cycle leads to polyploid cells, which are classified into mononuclear or binuclear polyploid cells depending on the number of nuclei. Polyploidy is common in plants and in animals. Physiologically, polyploidy and binucleation are differentiation markers and also features of the aging process. In fact, although they provide multiple copies of genes required for survival, a negative correlation between growth capacity and polyploidy has been reported, and thus, suppression or reversal of this phenomenon may be a growth advantage. On the other hand, unscheduled polyploidization may cause genomic instability that might lead to neoplastic aneuploidy. The aim of this rev…