Search results for "regulator"
showing 10 items of 1009 documents
New insights into the role of spermine in Arabidopsis thaliana under long-term salt stress
2010
Polyamines (putrescine, spermidine and spermine) are traditionally implicated in the response of plants to environmental cues. Free spermine accumulation has been suggested as a particular feature of long-term salt stress, and in the model plant Arabidopsis thaliana the spermine synthase gene (AtSPMS) has been reported as inducible by abscisic acid (ABA) and acute salt stress treatments. With the aim to unravel the physiological role of free spermine during salinity, we analyzed polyamine metabolism in A. thaliana salt-hypersensitive sos mutants (salt overlay sensitive; sos1-1, sos2-1 and sos3-1), and studied the salt stress tolerance of the mutants in spermine and thermospermine synthesis …
Cystic Fibrosis Transmembrane Conductance Regulator Interacts with Multiple Immunoglobulin Domains of Filamin A
2010
Mutations of the chloride channel cystic fibrosis transmembrane conductance regulator (CFTR) that impair its apical localization and function cause cystic fibrosis. A previous report has shown that filamin A (FLNa), an actin-cross-linking and -scaffolding protein, interacts directly with the cytoplasmic N terminus of CFTR and that this interaction is necessary for stability and confinement of the channel to apical membranes. Here, we report that the CFTR N terminus has sequence similarity to known FLNa-binding partner-binding sites. FLNa has 24 Ig (IgFLNa) repeats, and a CFTR peptide pulled down repeats 9, 12, 17, 19, 21, and 23, which share sequence similarity yet differ from the other FLN…
PHD3 regulates EGFR internalization and signalling in tumours
2014
Tumours exploit their hypoxic microenvironment to induce a more aggressive phenotype, while curtailing the growth-inhibitory effects of hypoxia through mechanisms that are poorly understood. The prolyl hydroxylase PHD3 is regulated by hypoxia and plays an important role in tumour progression. Here we identify PHD3 as a central regulator of epidermal growth factor receptor (EGFR) activity through the control of EGFR internalization to restrain tumour growth. PHD3 controls EGFR activity by acting as a scaffolding protein that associates with the endocytic adaptor Eps15 and promotes the internalization of EGFR. In consequence, loss of PHD3 in tumour cells suppresses EGFR internalization and hy…
In the Eye of the Beholder: How Regulatory Focus and Self-Efficacy Interact in Influencing Opportunity Recognition
2012
Although there is evidence that regulatory focus is associated with opportunity exploitation, there is a lack of research examining its role at the early stages of opportunity recognition. The present study makes two major contributions to address this gap. First, we demonstrate that entrepreneurs' promotion focus is positively related to opportunity recognition, whereas prevention focus is not significantly related to opportunity recognition. Second, integrating two theories of self-regulation – regulatory focus theory and self-efficacy theory – our findings reveal that a high promotion focus compensates for entrepreneurs' low levels of creative and entrepreneurial self-efficacy in opportu…
A note on validity in law and regulatory systems (position paper)
2012
This is position paper is located at the theoretical crossroads of several projects: CAPER, EU Grant Agreement 261712; CROWDSOURCING DER2012-39492-C02-0, SINTELNET FP7-ICT-2009-C-286380; CONSUMEDIA INNPACTO IPT-2011-1015-430000; CROWD-CRISSCONTROL IPT-2012-0968-390000. The notion of validity fulfils a crucial role in legal theory. The emerging Web 3.0 opens a new landscape where Semantic Web languages, legal ontologies, and the construction of Normative Multiagent Systems are built up to cover new regulatory needs. Conceptual models for complex regulatory systems shape the characteristic features of rules, norms and principles in different ways. This position paper outlines one of such mult…
Proteomic analysis reveals a role for Bcl2-associated athanogene 3 and major vault protein in resistance to apoptosis in senescent cells by regulatin…
2014
Senescence is a prominent solid tumor response to therapy in which cells avoid apoptosis and instead enter into prolonged cell cycle arrest. We applied a quantitative proteomics screen to identify signals that lead to therapy-induced senescence and discovered that Bcl2-associated athanogene 3 (Bag3) is up-regulated after adriamycin treatment in MCF7 cells. Bag3 is a member of the BAG family of co-chaperones that interacts with Hsp70. Bag3 also regulates major cell-signaling pathways. Mass spectrometry analysis of the Bag3 Complex revealed a novel interaction between Bag3 and Major Vault Protein (MVP). Silencing of Bag3 or MVP shifts the cellular response to adriamycin to favor apoptosis. We…
Cholesterol Starvation and Hypoxia Activate the FVII Gene via the SREBP1-GILZ Pathway in Ovarian Cancer Cells to Produce Procoagulant Microvesicles
2019
AbstractInteraction between the transcription factors, hypoxia-inducible factor (HIF1α and HIF2α) and Sp1, mediates hypoxia-driven expression of FVII gene encoding coagulation factor VII (fVII) in ovarian clear cell carcinoma (CCC) cells. This mechanism is synergistically enhanced in response to serum starvation, a condition possibly associated with tumor hypoxia. This transcriptional response potentially results in venous thromboembolism, a common complication in cancer patients by producing procoagulant extracellular vesicles (EVs). However, which deficient serum factors are responsible for this characteristic transcriptional mechanism is unknown. Here, we report that cholesterol deficien…
The effects of benzyladenine and meta-Topolin on in vitro sprouting and regrowth after encapsulation of C35 citrange [Citrus sinensis (L.) Osb. × Pon…
2016
C35, a hybrid of ‘Ruby Blood’ sweet orange and trifoliate orange, is a promising Citrus rootstock, mainly due to its resistance to Citrus Tristeza Virus (CTV), one of the most serious pathology affecting citriculture. The use of resistant rootstocks, such as citranges, is an efficient tool to fight this plant disease. This implies a continuous increasing demand of resistant rootstocks, in order to carry out the turnover of sour orange, still the most used Citrus rootstock in Italy. Micropropagation, can provide innovative solutions to obtain, in a short time and in a small space, a number of plants higher than traditional propagation. Micropropagation efficiency is also affected by the cult…
In vitro propagation of Lithodora rosmarinifolia (Ten.) Johnst., a rare endemic Sicilian shrub with potential as ornamental plant.
2007
Lithodora rosmarinifolia is a rare shrub endemic to the Mediterranean island of Sicily and its minor islands. Propagation of Lithodora rosmarinifolia by standard methods is difficult due to erratic seed production and low percentage of rooted cuttings. As the plant is suited for domestication, the possibility of establishing an efficient in vitro technique for propagation and conservation of this threatened species was investigated. Nodal segments from shoot tips were used as primary explants to establish in vitro culture. A 2 X 5 factorial experiment with five concentrations (0 to 17.74 mM) of N6-benzyladenine (BA) and two concentrations (0 and 2.46 mM) of indole-3-butyric acid (IBA) was u…
Effect of sodium dodecyl sulfate (SDS) on stress response in the Mediterranean mussel (Mytilus Galloprovincialis): Regulatory volumedecrease (Rvd) an…
2014
In this study the effects of an anionic surfactant, sodium dodecyl sulfate (SDS), are assessed on the Mediterranean mussel (. Mytilus galloprovincialis), exposed for 18 days at a concentration ranging from 0.1. mg/l to 1. mg/l. The effects are monitored using biomarkers related to stress response, such as regulatory volume decrease (RVD), and to oxidative stress, such as reactive oxygen species (ROS), endogenous antioxidant systems and Hsp70 levels. The results demonstrate that cells from the digestive gland of M. galloprovincialis, exposed to SDS were not able to perform the RVD owing to osmotic stress. Further, SDS causes oxidative stress in treated organisms, as demonstrated by the incre…