Search results for "report"
showing 10 items of 2364 documents
Specific sequence elements in the 5′ untranslated regions of rbcL and atpB gene mRNAs stabilize transcripts in the chloroplast of Chlamydomonas reinh…
2001
Using a series of point mutations in chimeric reporter gene constructs consisting of the 5' regions of the Chlamydomonas chloroplast rbcL or atpB genes fused 5' to the coding sequence of the bacterial uidA (GUS) gene, RNA-stabilizing sequence elements were identified in vivo in the 5' untranslated regions (5' UTRs) of transcripts of the chloroplast genes rbcL and atpB in Chlamydomonas reinhardtii. In chimeric rbcL 5' UTR:GUS transcripts, replacement of single nucleotides in the 10-nt sequence 5'-AUUUCCGGAC-3', extending from positions +38 to +47 relative to the transcripts' 5' terminus, shortened transcript longevity and led to a reduction in transcript abundance of more than 95%. A similar…
Changes in the 5?-untranslated region of the rbcL gene accelerate transcript degradation more than 50-fold in the chloroplast of Chlamydomonas reinha…
2003
Using uidA (beta-glucuronidase; GUS) reporter gene constructs, the 5'-untranslated region (UTR) of the Chlamydomonas chloroplast rbcL gene was screened by deletion and mutational analysis for the presence of a promoter element that previous studies implied to reside within the first 63 base pairs of the UTR. Deleting a large segment of the rbcL 5'UTR in a 3'--5' direction to position +36, changing the remaining 36 base pairs at the 5' end of the UTR, and increasing by five base pairs the distance between the rbcL 5'UTR and the basic promoter element located at position -10 did not abolish transcription from the basic rbcL promoter. It is concluded that the apparent loss of transcriptional a…
The 3'-UTR of the mRNA coding for the major protein kinase C substrate MARCKS contains a novel CU-rich element interacting with the mRNA stabilizing …
2003
The expression of the major protein kinase C substrate MARCKS (myristoylated alanine-rich C kinase substrate) is controlled by the stability of its mRNA. While the MARCKS mRNA is long living in quiescent fibroblasts (t1/2 = 14 h), its half-life time is drastically reduced (t1/2 = 2 h) in cells treated with phorbol esters to activate protein kinase C (PKC) or treated with growth factors. In a first step to study the underlying mechanism we identified both a cis-element on the MARCKS mRNA and the corresponding trans-acting factors. Fusing the complete 3'-UTR or specific regions of the 3'-UTR of the MARCKS gene to a luciferase reporter gene caused a drastic decrease in luciferase expression to…
3'-Untranslated regions of oxidative phosphorylation mRNAs function in vivo, as enhancers of translation
2000
Recent findings have indicated that the 3´-untranslated region (3´-UTR) of the mRNA encoding the β-catalytic subunit of the mitochondrial H+-ATP synthase has an in vitro translation-enhancing activity (TEA) [Izquierdo and Cuezva, Mol. Cell. Biol. (1997) 17, 5255–5268; Izquierdo and Cuezva, Biochem. J. (2000) 346, 849–855]. In the present work, we have expressed chimaeric plasmids that encode mRNA variants of green fluorescent protein in normal rat kidney and liver clone 9 cells to determine whether the 3´-UTRs of nuclear-encoded mRNAs involved in the biogenesis of mitochondria have an intrinsic TEA. TEA is found in the 3´-UTR of the mRNAs encoding the α- and β-subunits of the rat H+-ATP syn…
Report WS6: Immigration and new inhabitants in the Gateway Cities
2012
It is in the last years that the planning discipline has fully recognized immigration issues as decisive for their role both in the urban studies and in the policy design. Nowadays, in Italy and Europe, the shifting social composition of the cities asks the planner for new cognitive instruments and new strategies for the interventions on urban systems. The present report resumes the results of the workshop “Immigration and new inhabitants in the gateway cities” which took place inside the IX Biennial of Towns and Town Planners of Europe, held in Genoa in September 2011. In the two days of debate, a number of Italian and European examples has been presented: the report underlines three issue…
Giustizia ambientale e recupero delle aree verdi: il caso di Palermo
2021
L’urban shrinkage è diventata una questione centrale tanto nel dibattito scientifico quanto nell’agenda politica di diverse città europee. Secondo i dati EUROSTAT (2019), circa il 40% delle città europee con più di 200.000 abitanti stanno perdendo popolazione; una contrazione che si esplicita maggiormente, sotto il profilo spaziale, in una progressiva sottoutilizzazione del patrimonio edificato, così come nel contestuale incremento di aree e terreni in stato di abbandono. In questo quadro controverso di mutazione strutturale della città, così come l’abbiamo conosciuta, le politiche urbane alla prova in Europa tentano di declinare la decrescita quale occasione strategica di investimento sull…
Accelerometer-determined physical activity and self-reported health in a population of older adults (65-85 years): A cross-sectional study
2014
Published version of an article in the journal: BMC Public Health. Also available from the publisher at: http://dx.doi.org/10.1186/1471-2458-14-284 Open Access Background: The link between physical activity (PA) and prevention of disease, maintenance of independence, and improved quality of life in older adults is supported by strong evidence. However, there is a lack of data on population levels in this regard, where PA level has been measured objectively. The main aims were therefore to assess the level of accelerometer-determined PA and to examine its associations with self-reported health in a population of Norwegian older adults (65-85 years). Methods. This was a part of a national mul…
What’s the resolutive surgery for Pseudo-Ainhum in vohwinkel syndrome? A case report and review of the literature
2021
Vohwinkel Syndrome, also known as Keratoderma Hereditarium Mutilans, is an extremely rare dominant autosomal keratosis. It typically presents with “starfish” keratoses on the knuckles, palmoplantar keratoderma (PPK), hearing impairment and mutilating digital constriction bands (pseudoainhum) that cause strangulation, often leading to autoamputation of the affected digit. Both medical and surgical treatment haven’t shown to date consistent results, in the treatment of pseudoainhum. In this study we present the case of a woman with Vohwinkel syndrome who showed constriction bands causing ischemic changes of the 5th digit of the right hand for which she was treated with surgery. We also presen…
Post-mortem findings in vaccine-induced thrombotic thrombocytopenia
2021
Greinacher et al.1 and Schultz et al.2 were the first to independently report the main clinical and laboratory features of 11 and five respective patients from Germany, Austria and Norway who developed life-threatening thrombohemorrhagic complications 5 to 16 days after the administration of the first dose of the chimpanzee adenoviral vector vaccine ChAdOx1nCoV-19 against SARS-CoV-2 and COVID-19. Subsequently Scully et al.3 reported similar findings in 23 patients treated with the same vaccine in the United Kingdom. More recently, See et al.4 reported a case series of 12 patients from the USA with cerebral venous sinus thrombosis following the vaccination with Ad26.CoV2.S employing a human …
Augstskolu gada pārskatu diahroniskā analīze
2022
Komunikācija ir jebkuras organizācijas nozīmīga daļa, tai skaitā arī augstskolu. Valodas pielietojuma analīzes augstskolu diskursos līdz šim veiktas tikai mājaslapām un brošūrām; gadu pārskati nav pētīti. Bakalaura darba mērķis ir analizēt LU Attīstības stratēģijā (2015. – 2020.) norādīto septiņu vērtību atspoguļojumu 2018., 2019. un 2020. gada pārskatā. Darba teorētiskajā daļā tiek izmantota integrējošā literatūras pārskatu metode un empīriskajā daļā tiek apspriesta pētījumā veiktā satura analīze. Analīzes rezultāti norāda, ka visos trīs pārskatos cilvēku un sadarbības vērtības ir ar lielāko leksisko vienību skaitu, no kā tiek secināts, ka tās ir visvairāk uzsvērtās.