Search results for "response"
showing 10 items of 4136 documents
Good clinical response, remission, and predictors of remission in rheumatoid arthritis patients treated with tumor necrosis factor-α blockers: The GI…
2007
OBJECTIVE: To assess the prevalence of good clinical response and remission in rheumatoid arthritis (RA) patients with longstanding disease treated with anti-tumor necrosis factor-alpha (TNF-alpha) drugs at outpatient clinics. METHODS: Retrospective national study of 14 academic tertiary referral rheumatology medical centers. RA patients with a Disease Activity Score (DAS28) > 3.2 were defined as having active disease and could start TNF-alpha blockers. All patients received one TNF-alpha blocker plus methotrexate (10-20 mg/wk). At the third month the patients were categorized as responders or nonresponders, based on improvement of at least 0.25 of the Health Assessment Questionnaire (HAQ).…
Delivery of proteins into living cells by reversible membrane permeabilization with streptolysin-O
2001
The pore-forming toxin streptolysin O (SLO) can be used to reversibly permeabilize adherent and nonadherent cells, allowing delivery of molecules with up to 100 kDa mass to the cytosol. Using FITC-labeled albumin, 10 5 –10 6 molecules were estimated to be entrapped per cell. Repair of toxin lesions depended on Ca 2+ -calmodulin and on intact microtubules, but was not sensitive to actin disruption or to inhibition of protein synthesis. Resealed cells were viable for days and retained the capacity to endocytose and to proliferate. The active domains of large clostridial toxins were introduced into three different cell lines. The domains were derived from Clostridium difficile B-toxin and Clo…
Late Activation of Stress-activated Protein Kinases/c-Jun N-terminal Kinases Triggered by Cisplatin-induced DNA Damage in Repair-defective Cells
2011
Although stress-activated protein kinases/c-Jun N-terminal kinases (SAPK/JNK) are rapidly activated by genotoxins, the role of DNA damage in this response is not well defined. Here we show that the SEK1/MKK4-mediated dual phosphorylation of SAPK/JNK (Thr-183/Tyr-185) correlates with the level of cisplatin-DNA adducts at late times (16–24 h) after drug treatment in both human and mouse cells. Transfection of platinated plasmid DNA also caused SAPK/JNK activation. A defect in transcription-coupled nucleotide excision repair resting on a mutation in Cockayne syndrome group B protein promoted the late SAPK/JNK activation following cisplatin exposure. Signaling to SAPK/JNK was accompanied by act…
Metabotropic glutamate receptors activate phospholipase D in astrocytes through a protein kinase C-dependent and Rho-independent pathway.
2003
Metabotropic glutamate receptors (mGluRs) are G protein-coupled receptors that mediate phospholipase D (PLD) activation in brain, but the mechanism underlying this response remains unclear. Here we used primary cultures of astrocytes as a cell model to explore the mechanism that links mGluRs to PLD. Glutamate activated both phospholipase C (PLC) and PLD with equal potency and this effect was mimicked by L-cysteinesulfinic acid, a putative neurotransmitter previously shown to activate mGluRs coupled to PLD, but not PLC, in adult brain. PLD activation by glutamate was dependent on Ca(2+) mobilization and fully blocked by both protein kinase C (PKC) inhibitors and PKC down-regulation, suggesti…
La formation du comportement de plainte chez le consommateur: recherche exploratoire chez des usagers de restaurants
2008
La investigación que se presenta aborda el proceso de formación del comportamiento de queja del consumidor. El objetivo general es profundizar en la naturaleza de este tipo de respuesta a la insatisfacción, describiendo su formación a partir de la contribución de un conjunto de variables investigadas en la literatura. Partiendo de que el comportamiento de queja agrupa tres tipos de respuestas (de queja, a terceras partes y privadas), analizamos el efecto que tiene el nivel de insatisfacción, la probabilidad de éxito de la queja, la importancia de la situación y las actitudes hacia la queja. Las relaciones causales estudiadas entre estos determinantes y los comportamientos de queja han permi…
MicroRNAs in Extracellular Vesicles in Sweat Change in Response to Endurance Exercise
2020
Background: To date, microRNAs (miRs) carried in extracellular vesicles (EVs) in response to exercise have been studied in blood but not in non-invasively collectable body fluids. In the present study, we examined whether six exercise–responsive miRs, miRs-21, -26, -126, -146, -221, and -222, respond to acute endurance exercise stimuli of different intensities in sweat. Methods: We investigated the response of miRs isolated from sweat and serum EVs to three endurance exercise protocols: (1) maximal aerobic capacity (VO2max), (2) anaerobic threshold (AnaT), and (3) aerobic threshold (AerT) tests. Sauna bathing was used as a control test to induce sweating through increased body temperature i…
The Paracentrotus lividus metallothionein gene family: structure and expression.
2014
Metallothioneins are metal binding proteins that play a pivotal role in metal homeostasis and detoxification. Since their initial discovery, they have been extensively studied in a variety of organisms ranging from microbes to plants and animals. Organisms often possess multiple genes encoding metallothionein homologs with distinct properties, such as varying affinities for different metals, and in many cases different functions. Despite the plethora of available studies, very little information is known about sea urchin P. lividus MT (1, 2). We previously identified five Pl-MT embryonic cDNAs and we studied their induction after cadmium treatment (3). Now we studied their expression during…
Study on the effects of the adipose tissue-derived mesenchymal stem cell secretome on the innate inflammatory response
2018
Se ha demostrado que las células madre mesenquimales derivadas de tejido adiposo (MSC) ejercen efectos beneficiosos sobre algunas afecciones inflamatorias, que se han relacionado principalmente con la secreción de factores paracrinos. Por lo tanto, se han investigado las MSC como posibles herramientas terapéuticas para el tratamiento de enfermedades inflamatorias y autoinmunes. El secretoma de las MSC contiene factores solubles y componentes vesiculares conocidos como vesículas extracelulares (EV) que juegan un papel importante en diferentes procesos fisiológicos. En este trabajo, hemos investigado el potencial del medio acondicionado (CM) de ratón de las MSC para modular la respuesta infla…
Leveraging National Auditing Criteria to Implement Cybersecurity Safeguards for the Automotive Emergency Response Vehicles : A case study from Finland
2017
A modern Emergency Response Vehicle (ERV) is a combination of emergency services and functional mobile office on the wheels. The mobile office is aiming to leverage the benefits of fixed office while moving on the wheels. Researchers have observed that emergency response personnel including Law Enforcement Authorities (LEAs), Police and border guards, could be on the duty while having possibility to use same services compared to fixed office. On the one hand, demand of mobile office has significantly improved the emergency response services. On the other hand, emergency vehicle designers should rethink the demand of users. This resulted into modern standard emergency response vehicle with t…
From Country Girl in Southern Finland to Longitudinal Research into Alternatives to Aggression and Violence
2021
Lea Pulkkinen, born in Finland in 1939, is Emerita Professor of Psychology at the University of Jyväskylä (Finland). She is best known for creating the ongoing Jyväskylä Longitudinal Study of Personality and Social Development (JLSPSD). The study was specifically intended to test the hypothesis that the human brain allows for more variation in behavior than the simple ‘fight or flight’ response observed in animal studies of aggression. She further hypothesized that humans’ capacity for cognitive control over emotional behavior was the key factor involved in controlling aggressive behavior. These hypotheses led her to devise an impulse control model to depict behavioral alternatives, which s…