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showing 10 items of 2426 documents

Characterization of disease-specific cellular abundance profiles of chronic inflammatory skin conditions from deconvolution of biopsy samples

2019

Background Psoriasis and atopic dermatitis are two inflammatory skin diseases with a high prevalence and a significant burden on the patients. Underlying molecular mechanisms include chronic inflammation and abnormal proliferation. However, the cell types contributing to these molecular mechanisms are much less understood. Recently, deconvolution methodologies have allowed the digital quantification of cell types in bulk tissue based on mRNA expression data from biopsies. Using these methods to study the cellular composition of the skin enables the rapid enumeration of multiple cell types, providing insight into the numerical changes of cell types associated with chronic inflammatory skin c…

Keratinocytes0301 basic medicinePathologyMicroarraysBiopsyPATHOGENESISTranscriptome0302 clinical medicineDatabases GeneticLeukocytesATOPIC-DERMATITISGenetics (clinical)SkinPSORIASISmedicine.diagnostic_testintegumentary systemAtopic dermatitisDermismedicine.anatomical_structureDIFFERENTIATION030220 oncology & carcinogenesisChronic inflammatory skin diseasesResearch ArticleEXPRESSIONlcsh:Internal medicinemedicine.medical_specialtyCell typeGENESlcsh:QH426-470BiologyDENDRITIC CELLSDermatitis AtopicFlow cytometryMECHANISMS03 medical and health sciencesDermisPsoriasisBiopsyGeneticsmedicineHumanslcsh:RC31-1245SIGNATURESInflammationIDENTIFICATIONReproducibility of Resultsmedicine.diseaselcsh:Genetics030104 developmental biologyGene Expression RegulationChronic DiseaseSkin biopsyGene expressionEpidermis
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Validity and Reliability of the Leomo Motion-Tracking Device Based on Inertial Measurement Unit with an Optoelectronic Camera System for Cycling Peda…

2022

Background: The use of inertial measurement sensors (IMUs), in the search for a more ecological measure, is spreading among sports professionals with the aim of improving the sports performance of cyclists. The kinematic evaluation using the Leomo system (TYPE-R, Leomo, Boulder, CO, USA) has become popular. Purpose: The present study aimed to evaluate the reliability and validity of the Leomo system by measuring the angular kinematics of the lower extremities in the sagittal plane during pedaling at different intensities compared to a gold-standard motion capture camera system (OptiTrack, Natural Point, Inc., Corvallis, OR, USA). Methods: Twenty-four elite cyclists recruited from national a…

KinematicsHealth Toxicology and MutagenesisPublic Health Environmental and Occupational HealthReproducibility of ResultsCyclingCinemàticaIMUBicyclingBiomechanical PhenomenaMotion analysismotion analysis; cycling; kinematics; range of movement; IMURange of Motion ArticularRange of movementSportsInternational Journal of Environmental Research and Public Health; Volume 19; Issue 14; Pages: 8375
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An Existence Result for Fractional Kirchhoff-Type Equations

2016

The aim of this paper is to study a class of nonlocal fractional Laplacian equations of Kirchhoff-type. More precisely, by using an appropriate analytical context on fractional Sobolev spaces, we establish the existence of one non-trivial weak solution for nonlocal fractional problems exploiting suitable variational methods.

Kirchhoff typeApplied MathematicsFractional equations010102 general mathematicsMathematical analysisvariational methodsVariational methodAnalysiCritical point result01 natural sciencesFractional equationsFractional equationFractional calculus010101 applied mathematicscritical point resultsSimultaneous equations0101 mathematicsFractional equations variational methods critical point resultsAnalysisMathematicsZeitschrift für Analysis und ihre Anwendungen
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Early and midterm outcomes of bioresorbable vascular scaffolds for ostial coronary lesions: insights from the GHOST-EU registry.

2016

Aims: We aimed to investigate the outcomes of bioresorbable vascular scaffolds (BVS) in coronary ostial lesions. Ostial lesions represent a challenging angiographic subset, with higher event rates compared with non-ostial lesions. BVS might be associated with advantages over the long term, but their safety in this setting remains to be explored. Methods and results: Procedural and 12-month follow-up data from consecutive patients treated with BVS for lesions located at the ostium of the right (RCA), left anterior (LAD) or circumflex (LCX) coronary in 11 European centres were collected. The primary device-oriented endpoint was defined as a combination of cardiovascular death, target vessel m…

LCX (29Target lesionMale52%). Patients presenting with ostial lesions did not differ from the remaining cohort except for a higher incidence of prior revascularisation. Predilation was performed in 97% of the lesions (vs. 96% in non-ostialp= 0.035)medicine.medical_treatmentMyocardial Infarction304 patients with a mean age of 62 +/- 11years. There were 90 ostial lesions (5.8%) in 84 patients (6.4%) located at the ostial RCA (14Coronary Artery Disease030204 cardiovascular system & hematologyCoronary artery diseasebut their safety in this setting remains to be explored. Methods and results: Procedural and 12-month follow-up data from consecutive patients treated with BVS for lesions located at the ostium of the right (RCA)0302 clinical medicineAbsorbable Implants030212 general & internal medicineMyocardial infarctionCircumflexRegistriesTissue Scaffolds32%)Drug-Eluting StentsMiddle AgedThrombosisCoronary VesselsAims: We aimed to investigate the outcomes of bioresorbable vascular scaffolds (BVS) in coronary ostial lesions. Ostial lesions represent a challenging angiographic subset with higher event rates compared with non-ostial lesions. BVS might be associated with advantages over the long term but their safety in this setting remains to be explored. Methods and results: Procedural and 12-month follow-up data from consecutive patients treated with BVS for lesions located at the ostium of the right (RCA) left anterior (LAD) or circumflex (LCX) coronary in 11 European centres were collected. The primary device-oriented endpoint was defined as a combination of cardiovascular death target vessel myocardial infarction or target lesion revascularisation. The database included a total of 1549 lesions in 1304 patients with a mean age of 62 +/- 11years. There were 90 ostial lesions (5.8%) in 84 patients (6.4%) located at the ostial RCA (14; 16%) LCX (29; 32%) or LAD (47; 52%). Patients presenting with ostial lesions did not differ from the remaining cohort except for a higher incidence of prior revascularisation. Predilation was performed in 97% of the lesions (vs. 96% in non-ostial p= 0.618) post-dilation in 43% (versus 58% in the non-ostial group p= 0.008). At quantitative coronary angiography treatment of ostial lesions was associated with higher residual stenosis (30% [23-41] vs. 26% [20-37] p= 0.035) but no difference in minimum lumen diameter existed (p= 0.447). Follow-up data were available at 385 [362-465] days. The 12-month Kaplan-Meier estimated rates of scaffold thrombosis were 4.9% and 2.0% (ostial and non-ostial lesion groups respectively log-rank p= 0.005). The device-oriented composite endpoint occurred respectively in 12.6% and 4.6% at 12 months (log-rank p= 0.001). Treatment of ostial lesions was an independent predictor of this endpoint (p= 0.0025 HR 2.65 [1.41-4.97]).OstiumAims: We aimed to investigate the outcomes of bioresorbable vascular scaffolds (BVS) in coronary ostial lesions. Ostial lesions represent a challenging angiographic subsetTreatment Outcomein 12.6% and 4.6% at 12 months (log-rank p= 0.001). Treatment of ostial lesions was an independent predictor of this endpoint (p= 0.0025CardiologyFemale549 lesions in 1medicine.symptomCardiology and Cardiovascular MedicineAdultpost-dilation in 43% (versus 58% in the non-ostial groupmedicine.medical_specialtyor LAD (47HR 2.65 [1.41-4.97])but no difference in minimum lumen diameter existed (p= 0.447). Follow-up data were available at 385 [362-465] days. The 12-month Kaplan-Meier estimated rates of scaffold thrombosis were 4.9% and 2.0% (ostial and non-ostial lesion groupsrespectivelyLesion03 medical and health sciencesPercutaneous Coronary Interventionwith higher event rates compared with non-ostial lesions. BVS might be associated with advantages over the long termleft anterior (LAD) or circumflex (LCX) coronary in 11 European centres were collected. The primary device-oriented endpoint was defined as a combination of cardiovascular deathInternal medicinemedicineHumanstarget vessel myocardial infarction or target lesion revascularisation. The database included a total of 1Agedp= 0.008). At quantitative coronary angiographybusiness.industryPercutaneous coronary interventionp= 0.618)treatment of ostial lesions was associated with higher residual stenosis (30% [23-41] vs. 26% [20-37]log-rank p= 0.005). The device-oriented composite endpoint occurredmedicine.diseaseSurgery16%)businessEuroIntervention : journal of EuroPCR in collaboration with the Working Group on Interventional Cardiology of the European Society of Cardiology
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Autoantibody detection using indirect immunofluorescence on HEp-2 cells

2009

The detection of autoantibodies is an important element in the diagnosis and monitoring of disease progression in patients with autoimmune diseases. In laboratory diagnostic tests for connective tissue and autoimmune liver diseases, indirect immunofluorescence on HEp-2 cells plays a central role in a multistage diagnostic process. Despite the high quality of diagnostics, findings at different laboratories can differ considerably due to a lack of standardization, as well as subjective factors. The present paper formulates recommendations for the standardized processing and interpretation of the HEp-2 cell test for the detection of non-organ-specific (especially antinuclear) antibodies. It pr…

Laboratory ProcedureAnti-nuclear antibodymedicine.disease_causeImmunofluorescenceGeneral Biochemistry Genetics and Molecular BiologyAutoimmunityHistory and Philosophy of ScienceCell Line TumormedicineHumansFluorescent Antibody Technique IndirectAutoantibodiesbiologymedicine.diagnostic_testbusiness.industryGeneral NeuroscienceAutoantibodyReproducibility of ResultsReference StandardsLaboratory quality controlTiterAntibodies AntinuclearImmunologybiology.proteinAntibodybusiness
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Endotoxins in ophthalmic viscosurgical devices.

2003

Purpose To measure the endotoxin concentration (EC) of 25 commercially available, hyaluronic acid- and hydroxypropylmethylcellulose-based (HPMC) ophthalmic viscosurgical devices (OVDs). Methods The in vitro Limulus amebocyte lysate (LAL) assay, which indicates the presence of endotoxins originating from gram-negative bacteria, was used to determine the EC. The procedure was performed according to the European Pharmacopoeia/USP. EC including duplicate determinations, negative controls, dilution series with control standard endotoxin, dilution series with sample extract and positive sample control. Results 16 OVDs (Amvisc®, Amvisc® Plus, Biolon®, Coatel®, Healon®, Healon® GV, Healon®5, HPMC O…

LactoseLimulus testOphthalmologic Surgical ProceduresMethylcelluloseSensitivity and Specificity03 medical and health sciences0302 clinical medicineGram-Negative BacteriaOxazinesHumansConcentration factorHyaluronic AcidLimulus TestEndophthalmitisChromatographyPositive sampleChemistryReproducibility of ResultsGeneral MedicineDilutionEndotoxinsOphthalmologyLimulus amebocyte lysateImmunology030221 ophthalmology & optometry030217 neurology & neurosurgery
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Radiologic team performance index: A new paradigm in KPI evaluating radiology examination volumes department performance: Results of Sicilian regiona…

2018

Background: Efficient management of Radiologic Operative Units (ROU) because population health needs increase while healthcare systems provide less resources expendable in technologies and employee. Purpose: Create a Key Performance Indicator (KPI) defined Radiologic Team Performance Index (RTPI) able to compare the activity and efficiency of ROU operating under different environments and with different resources. Methodology/Approach: A survey was conducted in Sicilian Public Healthcare System on behalf of Sicilian Group of Italian Society of Medical Radiology (SIRM) for a total of 63 investigated ROU. Obtained informations concern workforce, emergency care organization, technologies avail…

Leadership and Managementbusiness.industry030503 health policy & servicesHealth PolicyPopulation healthmedicine.diseasePerformance resultsPerformance indexanalysis of impact use investments in radiology HTA imaging technology and geographic distribution impact KPI in radiology measurement activity volumes in radiology radiologic team performance assessment radiologic workforce supporting investments in public health03 medical and health sciences0302 clinical medicineMedicine030212 general & internal medicineMedical emergency0305 other medical sciencebusinesshuman activitieshealth care economics and organizationsHealthcare systemInternational Journal of Healthcare Management
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Validity and Reliability of a Single Question for Leisure-Time Physical Activity Assessment in Middle-Aged Women.

2020

Purpose: To investigate the validity and test–retest reliability of a single seven-level scale physical activity assessment question (SR-PA L7) and its three-level categorization (SR-PA C3). Methods: The associations of SR-PA L7 and C3 with accelerometer-measured leisure-time physical activity (ACC-LTPA) and with the results of four different physical performance tests (6-min walk [n = 733], knee extension [n = 695], vertical jump [n = 731], and grip force [n = 780]) were investigated among women aged 47–55 years participating in the Estrogenic Regulation of Muscle Apoptosis study (n = 795). The reliability was studied using Spearman correlations with 4-month test–retest period (n = 152). R…

Leisure timePhysical activityValidityPhysical Therapy Sports Therapy and RehabilitationWalkingtestaus03 medical and health sciencesVertical jump0302 clinical medicineLeisure Activitiesphysical activity measurementSurveys and QuestionnairesStatisticstest–retest reliabilityaccelerometryHumans030212 general & internal medicineself-reported physical activityReliability (statistics)MathematicsHand StrengthRehabilitationReproducibility of Results030229 sport sciencesRepeatabilityMiddle AgedPhysical FitnessScale (social sciences)Exercise TestFemaleGeriatrics and GerontologyGrip forceGerontologyhuman activitiesfyysinen aktiivisuusluotettavuusJournal of aging and physical activity
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Solvent for removing silicone oil from intraocular lenses: experimental study comparing various biomaterials.

2000

Abstract Purpose To evaluate the effectiveness of a perfluorocarbon—perfluorohexyloctane (PFHO)—in removing silicone oil from different intraocular lenses (IOLs) and determine whether any removal technique enhances the removal of silicone oil. Methods Six IOL materials were analyzed: standard poly(methyl methacrylate) (PMMA), heparin-surface-modified (HSM) PMMA, AcrySof, 2 polyHEMAs with different water contents, and a second-generation SLM-2 silicone. One or 2 μL of a highly viscous silicone oil (5700 cs) was applied to the center of each IOL optic. Perfluorohexyloctane (2 mL) was then used to remove the silicone oil by 1 of 3 techniques: immersion of the IOL in PFHO; irrigation of the sil…

Lens materialsmedicine.medical_specialtyMaterials scienceSurface Propertiesmedicine.medical_treatmentIntraocular lensBiocompatible Materialschemistry.chemical_compoundSiliconemedicineHumansPolymethyl MethacrylateSilicone OilsLenses IntraocularFluorocarbonsComputerized analysisBiomaterialAdhesivenessReproducibility of ResultsSensory SystemsSilicone oilSurgerySolventOphthalmologychemistryIntraocular lensesSolventsMethacrylatesSurgeryBiomedical engineeringJournal of cataract and refractive surgery
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The influence of storage method on the transparency of pig crystalline lens

2019

Este artículo se ha publicado de forma definitiva en: http://www.scielo.br/pdf/abo/v82n1/0004-2749-abo-82-01-0056.pdf Purpose: The porcine eye is frequently used as a research model. This paper analyzes the effect of different storage methods on the transparency of pig crystalline lens. Methods: A spectral transmission curve (from 220 to 780 nm) for the crystalline lens was determined experimentally after storage in different conditions: saline solution, formalin, castor oil, and freezing at -80°C. The total transmission in the visible spectrum, which was used as an index of transparency, was calculated from these curves. For comparative purposes, fresh lenses were evaluated and used as con…

Lens crystallineCastor OilTime FactorsMaterials scienceLightUltraviolet RaysSwineSpectral transmissionSuínosOrgan preservationAnalytical chemistryCrystalline lens.Cerdos.law.inventionResearch model03 medical and health sciences0302 clinical medicinelcsh:OphthalmologyReference ValueslawFormaldehydeLens CrystallineFreezingTotal transmissionmedicineAnimalsCristalino.CristalinoLens crystallineAnimaisReproducibility of ResultsOrgan PreservationGeneral MedicineSwine.eye diseasesLens (optics)Ophthalmologylcsh:RE1-994SpectrophotometryCastor oilModels Animal030221 ophthalmology & optometrysense organsCongelamentoPreservação de órgãosVisible spectrummedicine.drugArquivos Brasileiros de Oftalmologia
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