Search results for "rokotteet"
showing 9 items of 9 documents
Optimized production and purification of Coxsackievirus B1 vaccine and its preclinical evaluation in a mouse model.
2017
Coxsackie B viruses are among the most common enteroviruses, causing a wide range of diseases. Recent studies have also suggested that they may contribute to the development of type 1 diabetes. Vaccination would provide an effective way to prevent CVB infections, and the objective of this study was to develop an efficient vaccine production protocol for the generation of novel CVB vaccines. Various steps in the production of a formalin-inactivated Coxsackievirus B1 (CVB1) vaccine were optimized including the Multiplicity Of Infection (MOI) used for virus amplification, virus cultivation time, type of cell growth medium, virus purification method and formulation of the purified virus. Safety…
The Vaccination Concerns in COVID-19 Scale (VaCCS) : Development and validation
2022
Vaccines are highly effective in minimizing serious cases of COVID-19 and pivotal to managing the COVID-19 pandemic. Despite widespread availability, vaccination rates fall short of levels required to bring about widespread immunity, with low rates attributed to vaccine hesitancy. It is therefore important to identify the beliefs and concerns associated with vaccine intentions and uptake. The present study aimed to develop and validate, using the AMEE Guide, the Vaccination Concerns in COVID-19 Scale (VaCCS), a comprehensive measure of beliefs and concerns with respect to COVID-19 vaccines. In the scale development phase, samples of Australian (N = 53) and USA (N = 48) residents completed a…
A hexavalent Coxsackievirus B vaccine is highly immunogenic and has a strong protective capacity in mice and nonhuman primates
2020
A proof-of-concept study shows the generation of an immunogenic hexavalent Coxsackie B virus vaccine that prevents disease.
Structural Insight into CVB3-VLP Non-Adjuvanted Vaccine
2020
Coxsackievirus B (CVB) enteroviruses are common pathogens that can cause acute and chronic myocarditis, dilated cardiomyopathy, aseptic meningitis, and they are hypothesized to be a causal factor in type 1 diabetes. The licensed enterovirus vaccines and those currently in clinical development are traditional inactivated or live attenuated vaccines. Even though these vaccines work well in the prevention of enterovirus diseases, new vaccine technologies, like virus-like particles (VLPs), can offer important advantages in the manufacturing and epitope engineering. We have previously produced VLPs for CVB3 and CVB1 in insect cells. Here, we describe the production of CVB3-VLPs with enhanced pro…
Credibility of online comments in the HPV vaccination discussion in Finnish online forums
2016
Internet ja lukuisat online-kanavat ja -foorumit ovat mahdollistaneet reaaliaikaisen kom-mentoinnin lähes jokaiselle ja näin yleisöistä ja sisällön kuluttajista on tullut sisällöntuottajia. Sisällöt voivat myös ohjailla kuluttajien käyttäytymistä. Kuluttajien tuottaman sisällön suuri määrä on johtanut siihen, että online-sisältöjen luotettavuuden arvioimisesta on tullut entistä tärkeämpää ja organisaatioiden täytyy ennakoida ja hallita heitä käsittelevää verkkokeskustelua. Tavallisten ihmisten kirjoittamat word of mouth -arviot verkossa koetaan usein luotettavammiksi kuin markkinointitekstit ja mainonta. Lisäksi yksilöiden välinen verkkokeskustelu on usein empaattisempaa kuin vastaava viran…
Predicting COVID-19 booster vaccine intentions.
2022
Achieving broad immunity through vaccination is a cornerstone strategy for long-term management of COVID-19 infections, particularly the prevention of serious cases and hospitalizations. Evidence that vaccine-induced immunity wanes over time points to the need for COVID-19 booster vaccines, and maximum compliance is required to maintain population-level immunity. Little is known of the correlates of intentions to receive booster vaccines among previously vaccinated individuals. The present study applied an integrated model to examine effects of beliefs from multiple social cognition theories alongside sets of generalized, stable beliefs on individuals' booster vaccine intentions. US residen…
Formalin treatment increases the stability and immunogenicity of coxsackievirus B1 VLP vaccine
2019
Type B Coxsackieviruses (CVBs) are a common cause of acute and chronic myocarditis, dilated cardiomyopathy and aseptic meningitis. However, no CVB-vaccines are available for human use. We have previously produced virus-like particles (VLPs) for CVB3 with a baculovirus-insect cell production system. Here we have explored the potential of a VLP-based vaccine targeting CVB1 and describe the production of CVB1-VLPs with a scalable VLP purification method. The developed purification method consisting of tangential flow filtration and ion exchange chromatography is compatible with industrial scale production. CVB1-VLP vaccine was treated with UV-C or formalin to study whether stability and immuno…
A comparative study of the effect of UV and formalin inactivation on the stability and immunogenicity of a Coxsackievirus B1 vaccine
2019
Type B Coxsackieviruses (CVBs) belong to the enterovirus genus, and they cause both acute and chronic diseases in humans. CVB infections usually lead to flu-like symptoms but can also result in more serious diseases such as myocarditis, aseptic meningitis and life-threatening multi-organ infections in young infants. Thus, CVBs have long been considered as important targets of future vaccines. We have previously observed CVB1 capsid disintegration and virus concentration decrease with 12-day long formalin inactivation protocol. Here a scalable ion exchange chromatography purification method was developed, and purified CVB1 was inactivated with UV-C or formalin. Virus morphology and concentra…
Säännöllinen liikunta tehostaa todennäköisesti koronarokotteen vaikutusta
2022
Liikunta vahvistaa tutkitusti rokotusten tehoa. Liikuntaharrastus pienentää myös riskiä sairastua vakavaan koronatautiin. nonPeerReviewed