Search results for "rt-PCR"

showing 10 items of 44 documents

Molecular characterization of cucumber mosaic virus isolates infecting ornamental species cultivated in the botanical garden of Bologna university.

2006

During an epidemiological survey carried out in the Botanical Garden at Bologna University, Cucumber mosaic virus (CMV) has been detected by applying PAS-ELISA technique infecting some ornamental species exhibiting a severe symptomatology on the leaves. Datura innoxia Miller. showed mosaic and leaf-curling; Globularia nudicaulis L. produced narrowed leaves with a yellow mosaic and/or variegation; Eupatorium cannabinum L. showed a systemic chlorotic and/or yellow mosaic and stunting. Reverse transcription-polymerase chain reaction (RT-PCR) and single strand conformation polymorphism (SSCP) were employed to characterise these CMV isolates. Total RNA was extracted from symptomatic leaf samples…

ORNAMENTAL PLANTSBOLOGNA UNIVERSITYbotanical gardenRT-PCRCMVfood and beveragesSYMPTOMATOLOGYSettore AGR/12 - Patologia VegetalePIANTE ORNAMENTALIISOLATI
researchProduct

Proposal of a Citrus translational genomic approach for early and infield detection of Flavescence dorée in Vitis

2014

Flavescence dore´e (FD) is one of the most widely known grapevine yellows disease and one of the most unabated worldwide in the viticulture sector. In this paper, we outline a strategy for developing an integrated system of technologies to enable rapid, early disease FD detection and diagnosis. We propose the deployment of a newly developed sensor device, the differential mobility spectrometer (DMS), which has shown positive results with a similar vector-borne disease in Citrus. We have previously demonstrated that the gas chromatograph DMS (GC/DMS) can distinguish various citrus diseases, and the system may also allow detection of volatile organic compound (VOC) signals from a tree of othe…

0106 biological sciences0301 basic medicinePlant ScienceComputational biologyBiology01 natural sciences03 medical and health sciencesSettore AGR/07 - Genetica AgrariaBotanyProfile analysisPlant systemEcology Evolution Behavior and SystematicsDifferential mobility spectrometer early detection Flavescence dore´e phytoplasma qRT-PCR ELISA VitisfungiEarly diseasefood and beveragesSettore AGR/12 - Patologia VegetaleGrapevine yellowsSettore AGR/02 - Agronomia E Coltivazioni ErbaceeSettore AGR/03 - Arboricoltura Generale E Coltivazioni Arboree030104 developmental biologySettore AGR/14 - PedologiaFlavescence doréeGas chromatographyGas chromatography–mass spectrometryDisease transmission010606 plant biology & botany
researchProduct

Detection and identification Fabavirus species by one-step RT-PCR and multiplex RT-PCR

2014

The genus Fabavirus of the family Secoviridae comprises a group of poorly characterized viruses. To date, only five species have been described: Broad bean wilt virus 1 (BBWV-1), Broad bean wilt virus 2 (BBWV-2), Lamium mild mosaic virus (LMMV), Gentian mosaic virus (GeMV) and Cucurbit mild mosaic virus (CuMMV). The development is described of two RT-PCR procedures for the detection and identification of Fabavirus species: a one-step RT-PCR using a single pair of conserved primers for the detection of all fabaviruses, and a one-step multiplex RT-PCR using species-specific primers for the simultaneous detection and identification of the above-mentioned species of the genus Fabavirus. These m…

Time FactorsbiologyReverse Transcriptase Polymerase Chain ReactionBBWV-1 BBWV-2 GeMV CuMMV LMMV detection diagnosis multiplex RT-PCR conserved primersLamium mild mosaic virusSettore AGR/12 - Patologia VegetaleDetection Diagnosis Conserved primersbiology.organism_classificationSensitivity and SpecificityVirologyFabavirusBroad bean wilt virusReal-time polymerase chain reactionGenusVirologyPlant virusSecoviridaeRNA ViralIdentification (biology)MultiplexDNA PrimersPlant Diseases
researchProduct

Implementation of Sample Pooling Procedure Using a Rapid SARS-CoV-2 Diagnostic Real-Time PCR Test Performed Prior to Hospital Admission of People wit…

2021

Reliability, accuracy, and timeliness of diagnostic testing for SARS-CoV-2 infection have allowed adequate public health management of the disease, thus notably helping the timely mapping of viral spread within the community. Furthermore, the most vulnerable populations, such as people with intellectual disability and dementia, represent a high-risk group across multiple dimensions, including a higher prevalence of pre-existing conditions, lower health maintenance, and a propensity for rapid community spread. This led to an urgent need for reliable in-house rapid testing to be performed prior to hospital admission. In the present study, we describe a pooling procedure in which oropharyngeal…

medicine.medical_specialtyHealth Toxicology and MutagenesisPoolingRT-PCRsample poolingSample (statistics)DiseaseReal-Time Polymerase Chain ReactionSensitivity and SpecificityIntellectual DisabilityIntellectual disabilitymedicineHumansDementiaHospital admission RT-PCR Sample pooling SARS-CoV-2 Sensitivity Hospitals Humans Real-Time Polymerase Chain Reaction Reproducibility of Results SARS-CoV-2 Sensitivity and Specificity COVID-19 Intellectual DisabilitySARS-CoV-2business.industryBrief ReportPublic healthRPublic Health Environmental and Occupational HealthCOVID-19Reproducibility of Resultssensitivitymedicine.diseaseHospitalsTest (assessment)hospital admissionEmergency medicineMedicineSample collectionbusinessInternational Journal of Environmental Research and Public Health
researchProduct

CHARACTERIZATION OF A STRAIN OF PEPINO MOSAIC VIRUS FOUND IN SICILY

2006

During winter 2005, an unusual virus-like yellow leaf disorder associated to interveinal leaf yellowing and marbling on fruits, was observed in some greenhouse tomato crops in Sicily, exactly in province of Ragusa. Two leaves from 250 symptomatic tomato plants were serologically tested by DAS-ELISA technique using the sera to Tomato spotted wilt virus (TSWV), Impatiens necrotic spot virus (INSV), Tobacco mosaic virus (TMV), Cucumber mosaic virus (CMV) and Pepino mosaic virus (PepMV). Of the 250 samples tested, 215 were positive to PepMV presence. This virus was mechanically transmitted to Cucumis sativus “Cubit”, Datura metel, Nicotiana benthamiana and tomato “Rio Grande”. The host range of…

PEPINO MOSAIC VIRUSfungiRT-PCRfood and beveragesTOMATOEPIDEMIOLOGYPepMVSICILY
researchProduct

Mboat7 down-regulation by hyper-insulinemia induces fat accumulation in hepatocytes.

2020

Background: Naturally occurring variation in Membrane-bound O-acyltransferase domain-containing 7 (MBOAT7), encoding for an enzyme involved in phosphatidylinositol acyl-chain remodelling, has been associated with fatty liver and hepatic disorders. Here, we examined the relationship between hepatic Mboat7 down-regulation and fat accumulation. Methods: Hepatic MBOAT7 expression was surveyed in 119 obese individuals and in experimental models. MBOAT7 was acutely silenced by antisense oligonucleotides in C57Bl/6 mice, and by CRISPR/Cas9 in HepG2 hepatocytes. Findings: In obese individuals, hepatic MBOAT7 mRNA decreased from normal liver to steatohepatitis, independently of diabetes, inflammatio…

Research paperTGFβ Transforming Growth Factor BetaIntracellular SpaceCRISPR Clustered Regularly Interspaced Short Palindromic RepeatshHEPS Human HepatocytesMice0302 clinical medicineLPIAT1DAG Diacylglyceroli.p. Intraperitonealmedia_commonFatty AcidsGeneral Medicine3. Good health030220 oncology & carcinogenesisHOMA-IR homeostasis Model Assessment of Insulin ResistanceMPO morpholinolcsh:Medicine (General)medicine.medical_specialtyPE Phosphatidyl-EthanolamineNashGeneral Biochemistry Genetics and Molecular Biology03 medical and health sciencesTNFα tumor Necrosis Factor AlphaLDL Low Density LipoproteinsHyperinsulinismNAFLDSD Standard Dietmedia_common.cataloged_instanceHumansCPT1 Carnitine Palmitoyltransferase IPhosphatidylinositolGene SilencingEuropean unionVLDL Very Low Density Lipoproteinlcsh:RhHSC Human Hepatic Stellate Cellsmedicine.diseaseLipid MetabolismOA Oleic AcidCI Confidence IntervalMboat7 Membrane bound O-acyltransferase domain containing 7MCD methionine choline deficient diet030104 developmental biologyEndocrinologychemistryCDP Cytidine-DiphosphateFOXO1 Forkhead Box protein O1NAFLD nonalcoholic fatty liver diseaseSteatohepatitisBMI Body Mass IndexCL CardiolipinAcyltransferases0301 basic medicineAlcoholic liver diseaseCXCL10 C-X-C Motif Chemokine 10lcsh:Medicinechemistry.chemical_compoundNon-alcoholic Fatty Liver DiseaseIFG Impaired Fasting GlucoseAPOB Apolipoprotein BNonalcoholic fatty liver diseasePIP Phosphatidyl-Inositol-PhosphateSteatohepatitisqRT-PCR quantitative Real Time Polymerase Chain ReactionMice Knockoutlcsh:R5-920ORO Oil Red O StainingPI PhosphatidylinositolFatty liverTM6SF2 Transmembrane 6 Superfamily Member 2PhospholipidTAG TriglyceridesNASH Nonalcoholic SteatohepatitisLipogenesisLPA Lyso-Phosphatidic AcidPhosphatidylinositolSignal TransductionPS Phosphatidyl-SerinePA Palmitic AcidALD alcoholic liver diseasePC Phosphatidylcholinei.v. IntravenousFATP1 Fatty Acid Transport Protein 1Models BiologicalInternal medicinemedicineAnimalsNonalcoholic fatty liver diseasePPARα Peroxisome Proliferator-Activated Receptor alphaObesityG3P Glyceraldehyde-3-PhosphateSREBP1c Sterol Regulatory Element-Binding Protein 1HDL High Density Lipoproteinsbusiness.industryPI3K Phosphatidylinositol 3 KinaseMembrane ProteinsNHEJ Non-Homologues End JoiningPNPLA3 Patatin-like Phospholipase Domain-containing-3MTTP Microsomal Triglyceride Transfer ProteinLPIAT1 Lysophosphatidylinositol Acyltransferase 1TMC4 Transmembrane Channel-Like 4Disease Models AnimalGene Expression RegulationHepatocytesFOXA2 Forkhead Box A2mTOR mammalian target of RapamycinSteatosisInsulin ResistancebusinessPG Phosphatidyl-GlycerolFABP1 Fatty Acid-Binding Protein 1 FAS Fatty Acid SynthaseT2DM Type 2 Diabetes MellitusEBioMedicine
researchProduct

Polymerase Chain Reaction (PCR)

2008

Polymerase Chain Reaction RT-PCRSettore BIO/10 - BiochimicaReal Time PCR.
researchProduct

Comparison of the BD Directigen Flu A+B Kit and the Abbott TestPack RSV with a multiplex RT-PCR ELISA for rapid detection of influenza viruses and re…

2005

ABSTRACTThe Directigen Flu A+B enzyme immunoassay and the Abbott TestPack RSV enzyme immunoassay were each compared with a multiplex RT-PCR ELISA by testing 635 nasopharyngeal aspirates collected from children aged < 16 years who had been hospitalised with acute respiratory tract infection during the epidemic season 2002–2003. In this study, the sensitivity of the Directigen Flu A+B assay was unacceptably low (29.3% and 10.0%, respectively) for the detection of influenza A and B viruses. The sensitivity of the Abbott TestPack RSV assay (77.4%) was acceptable and in agreement with the multiplex RT-PCR ELISA.

Microbiology (medical)Paramyxoviridaerespiratory syncytial virusrespiratory tract infectionEnzyme-Linked Immunosorbent AssayPolymerase Chain ReactionSensitivity and Specificityinfluenza virusVirusAssaysMicrobiologyPneumovirinaeNasopharynxMultiplex polymerase chain reactionmedicineMultiplexProspective StudiesMononegaviralesbiologymedicine.diagnostic_testReverse Transcriptase Polymerase Chain Reactionvirus diseasesGeneral Medicinebiology.organism_classificationenzyme immunoassayVirologyInfluenza B virusInfectious Diseasesmultiplex RT-PCRInfluenza A virusRespiratory Syncytial Virus HumanImmunoassayReagent Kits DiagnosticViral diseaseClinical Microbiology and Infection
researchProduct

CD40 activation in human pancreatic islets and ductal cells.

2008

Aims/hypothesis: CD40 expression on non-haematopoietic cells is linked to inflammation. We previously reported that CD40 is expressed on isolated human and non-human primate islets and its activation results in secretion of IL-8, macrophage inflammatory protein 1-beta (MIP-1β) and monocyte chemoattractant protein-1 (MCP-1) through nuclear factor-κB and extracellularly regulated kinases 1/2 pathways. The objective of this study was to identify the pattern of gene expression, and to study viability and functionality affected by CD40-CD40 ligand (CD40L) interaction in human islets. Furthermore, we have studied the CD40-mediated cytokine/chemokine profile in pancreatic ductal cells, as they are…

AdultChemokinemedicine.medical_specialtyDuctal cellsCell SurvivalEndocrinology Diabetes and Metabolismmedicine.medical_treatmentChemokine CXCL1CD40 Chemokines Cytokines Ductal cells Inflammation Insulin Islets of Langerhans Microarray Quantitative RT-PCRCD40 LigandEnzyme-Linked Immunosorbent AssayIslets of LangerhansYoung AdultInternal medicineInternal MedicinemedicineHumansCD40 AntigensMacrophage inflammatory proteinOligonucleotide Array Sequence AnalysisCD40biologySettore BIO/16 - Anatomia UmanaReverse Transcriptase Polymerase Chain ReactionPancreatic isletsPancreatic DuctsMiddle AgedFlow CytometryMolecular biologyCXCL1CXCL2Endocrinologymedicine.anatomical_structureCytokinebiology.proteinCytokinesChemokinesDiabetologia
researchProduct

Expression in Streptomyces lividans of Nonomuraea genes cloned in an artificial chromosome

2004

A bacterial artificial chromosomal library of Nonomuraea sp. ATCC39727 was constructed using Escherichia coli-Streptomyces artificial chromosome (ESAC) and screened for the presence of dbv genes known to be involved in the biosynthesis of the glycopeptide A40926. dbv genes were cloned as two large, partially overlapping, fragments and transferred into the host Streptomyces lividans, thus generating strains S. lividansColon, two colonsNmESAC50 and S. lividansColon, two colonsNmESAC57. The heterologous expression of Nonomuraea genes in S. lividans was successfully demonstrated by using combined RT-PCR and proteomic approaches. MALDI-TOF analysis revealed that a Nonomuraea ABC transporter is e…

DNA BacterialChromosomal library of Nonomuraea sp. ATCC39727Escherichia coli–Streptomyces artificial chromosome (ESAC)RT-PCRMolecular cloningApplied Microbiology and BiotechnologyStreptomycesGenetic analysisThiostreptonchemistry.chemical_compoundActinomycetalesChromosomes ArtificialCloning MolecularA40926GeneRegulator geneGeneticsGenomic LibrarybiologyMALDI-TOF mass spectrometryPromoterGeneral Medicinebiology.organism_classificationStreptomycesdbv gene cluster2D-PAGEchemistryGenes BacterialHeterologous expressionHeterologous expressionPulsed field gel electrophoresidalbavancinBiotechnologyApplied Microbiology and Biotechnology
researchProduct