Search results for "scaffold"

Effect of surface PEG content on the viability and cell adhesion in PLA/PEG porous scaffolds

2011

Scaffold con gradiente della dimensione dei pori per la migrazione selettiva di cellule eterotipiche

2014

POROUS SCAFFOLDS BASED ON PLLA/FUNCTIONALISED POLYMERS BLENDS PRODUCED BY THERMALLY INDUCED PHASE SEPARATION

2014

2020

In Bone Tissue Engineering (BTE), autologous bone-regenerative cells are combined with a scaffold for large bone defect treatment (LBDT). Microporous, polylactic acid (PLA) scaffolds showed good healing results in small animals. However, transfer to large animal models is not easily achieved simply by upscaling the design. Increasing diffusion distances have a negative impact on cell survival and nutrition supply, leading to cell death and ultimately implant failure. Here, a novel scaffold architecture was designed to meet all requirements for an advanced bone substitute. Biofunctional, porous subunits in a load-bearing, compression-resistant frame structure characterize this approach. An o…

Channeled scaffolds implanted in adult rat brain.

2012

Scaffolds with aligned channels based on acrylate copolymers, which had previously demonstrated good com- patibility with neural progenitor cells were studied as coloniz- able structures both in vitro with neural progenitor cells and in vivo, implanted without cells in two different locations, in the cortical plate of adult rat brains and close to the subven- tricular zone. In vitro, neuroprogenitors colonize the scaffold and differentiate into neurons and glia within its channels. When implanted in vivo immunohistochemical analysis by confocal microscopy for neural and endothelial cells markers demonstrated that the scaffolds maintained continuity with the surrounding neural tissue and wer…

GENERATION OF PREVASCULARIZED PLLA BIODEGRADABLE SCAFFOLDS BY DIP DRAWING AND DIFFUSION INDUCED PHASE SEPARATION (DIPS).

2009

A critical obstacle in tissue engineering is to develop a massive structure of living cells upon transfer from the in vitro culture conditions into the host in vivo. A vascular network is required to supply essential nutrients, including oxygen, remove metabolic waste products and provide a biochemical communication “highway”. For these reasons to build an implantable structure in which vessel formation (angiogenesis) take place is mandatory. PLLA scaffolds usable in vascular tissue engineering were generated by dip-coating via Diffusion Induced Phase Separation (DIPS) technique. The scaffolds, with a vessel-like shape, were obtained by performing a DIPS process around a nylon fibre whose d…

“ One Ring to Bind Them All ”—Part I: The Efficiency of the Macrocyclic Scaffold for G-Quadruplex DNA Recognition

2010

International audience; Macrocyclic scaffolds are particularly attractive for designing selective G-quadruplex ligands essentially because, on one hand, they show a poor affinity for the "standard" B-DNA conformation and, on the other hand, they fit nicely with the external G-quartets of quadruplexes. Stimulated by the pioneering studies on the cationic porphyrin TMPyP4 and the natural product telomestatin, follow-up studies have developed, rapidly leading to a large diversity of macrocyclic structures with remarkable-quadruplex binding properties and biological activities. In this review we summarize the current state of the art in detailing the three main categories of quadruplex-binding …

Using Polymeric Scaffolds for Vascular Tissue Engineering

2014

With the high occurrence of cardiovascular disease and increasing numbers of patients requiring vascular access, there is a significant need for small-diameter (<6 mm inner diameter) vascular graft that can provide long-term patency. Despite the technological improvements, restenosis and graft thrombosis continue to hamper the success of the implants. Vascular tissue engineering is a new field that has undergone enormous growth over the last decade and has proposed valid solutions for blood vessels repair. The goal of vascular tissue engineering is to produce neovessels and neoorgan tissue from autologous cells using a biodegradable polymer as a scaffold. The most important advantage of …

Bio-affinity of MC3T3-E1 osteoblastic cells with polymeric scaffold in Poly L-Lactid Acid (PLLA) for bone substitution.

2009

Damage to an organ or tissue remains a problem despite advances in medical technology. Available treatments include organs transplantation, surgical reconstruction such as mechanical devices. However, all these methodologies have several contraindications. In recent years, tissue engineering methods is considered as means to replace diseased or damaged organs. For these kind of application, the choice of scaffolding material is crucial to the success of the technique. In the function of the application, the synthetic scaffolds should meet several criteria, including: good biocompatibility, sufficient mechanical properties, and adequate biodegradability. The aim of our study was to evaluate …

Biosilica-loaded poly(ϵ-caprolactone) nanofibers mats provide a morphogenetically active surface scaffold for the growth and mineralization of the os…

2014

Bioprinting/3D cell printing procedures for the preparation of scaffolds/implants have the potential to revolutionize regenerative medicine. Besides biocompatibility and biodegradability, the hardness of the scaffold material is of critical importance to allow sufficient mechanical protection and, to the same extent, allow migration, cell–cell, and cell–substrate contact formation of the matrix-embedded cells. In the present study, we present a strategy to encase a bioprinted, cell-containing, and soft scaffold with an electrospun mat. The electrospun poly(e-caprolactone) (PCL) nanofibers mats, containing tetraethyl orthosilicate (TEOS), were subsequently incubated with silicatein. Silicate…