Search results for "screening"

showing 10 items of 1150 documents

Optimization of anti-proliferative activity using a screening approach with a series of bis-heterocyclic G-quadruplex ligands

2013

Abstract Using a phenotypic screening and SAR optimization approach, a phenyl-bis-oxazole derivative has been identified with anti-proliferative activity, optimized with the use of a panel of cancer cell lines. The lead compound was synthesized by means of a short and effective two-step synthesis using Pd-catalyzed direct arylation. The compound stabilizes several quadruplex DNA sequences including a human telomeric DNA and one from the promoter of the HSP90 gene, although the structure–activity relationships of the series are not obviously related to the quadruplex binding.

Phenotypic screeningClinical BiochemistryPharmaceutical ScienceG-quadruplexLigandsBiochemistrychemistry.chemical_compoundInhibitory Concentration 50Structure-Activity RelationshipHeterocyclic CompoundsCell Line TumorDrug DiscoveryHumansMolecular BiologyGeneCell ProliferationOrganic ChemistryCombinatorial chemistrySmall moleculeSettore CHIM/08 - Chimica FarmaceuticaG-QuadruplexeschemistryMolecular MedicineHuman genomeQuadruplex Anti-proliferative Phenotypic screening Telomerase OxazolesDrug Screening Assays AntitumorLead compoundDerivative (chemistry)DNA
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Maternal phenylketonuria in two Sicilian families identified by maternal blood phenylalanine level screening and identification of a new phenylalanin…

1999

not available

Phenylketonuria MaternalPhenylalanine hydroxylasephenylalanine 4 monooxygenasePhenylalanineGene mutationMaternal bloodNeonatal ScreeningPregnancyPhenylketonuriasMedicineHumansMaternal phenylketonuriaGenetic TestingPhenylalanine levelGeneticsbiologybusiness.industryInfant NewbornPhenylalanine HydroxylasePedigreeItalyPediatrics Perinatology and Child HealthMutationbiology.proteinIdentification (biology)FemalebusinessEuropean journal of pediatrics
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Selective Excitation of Guided Waves into Bone Phantoms by a Time-Delayed Laser Diode Array

2012

We excite selectively the Ao and So plate mode into an acrylic bone phantom using a laser diode array. This is done by controlling the time delay between the signals driving the array elements. Selective mode generation is important because it can potentially increase the sensitivity of in vivo osteoporosis screening.

Photoacoustic effect0303 health sciencesMaterials scienceLaser diodebusiness.industry030209 endocrinology & metabolismSelective excitationImaging phantomlaw.inventionOsteoporosis screeningUltrasonic imaging03 medical and health sciences0302 clinical medicineTime delayedOptics030301 anatomy & morphologylawbusinessSensitivity (electronics)2012 Symposium on Photonics and Optoelectronics
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DNA-binding and in vitro cytotoxic activity of platinum(II) complexes of curcumin and caffeine

2019

Abstract Three Pt(II) complexes containing the natural ligands curcumin and caffeine, namely [Pt(curc)(PPh3)2]Cl (1), [PtCl(curc)(DMSO)] (2) (curc = deprotonated curcumin) and trans-[Pt(caffeine)Cl2(DMSO)] (3), were synthesized and fully characterized. The data obtained suggest that, for both 1 and 2, the anion of curcumin is coordinated to the platinum ion via the oxygen atoms of the β-diketonate moiety. Spectroscopic features reveal that in 2 and 3, a DMSO molecule is S-bonded to the metal centre. For 3, all data indicate a square-planar geometry formed by a 9-N bonded caffeine, two trans chloride anions and a DMSO. The three complexes undergo changes in solution upon incubation for 24 h;…

PhotoactivationCurcuminCytotoxicityIntercalation (chemistry)chemistry.chemical_elementCaffeine; Curcumin; Cytotoxicity; DNA interaction; Natural ligands; Photoactivation; Platinum(II) complexAntineoplastic Agents010402 general chemistryLigands01 natural sciencesBiochemistryMedicinal chemistryNucleobaseInorganic Chemistrychemistry.chemical_compoundDrug StabilityCoordination ComplexesCaffeineCell Line TumorMoietyMoleculeAnimalsHumansPlatinumMolecular Structure010405 organic chemistryDNA0104 chemical sciencesDNA interactionchemistryCurcuminPlatinum(II) complexCattleCaffeine Curcumin Cytotoxicity DNA interaction Natural ligands Photoactivation Platinum(II) complexCisplatinDrug Screening Assays AntitumorSelectivityPlatinumNatural ligandsCis–trans isomerism
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$^{222}$Rn emanation measurements for the XENON1T experiment

2021

The selection of low-radioactive construction materials is of utmost importance for the success of low-energy rare event search experiments. Besides radioactive contaminants in the bulk, the emanation of radioactive radon atoms from material surfaces attains increasing relevance in the effort to further reduce the background of such experiments. In this work, we present the $^{222}$Rn emanation measurements performed for the XENON1T dark matter experiment. Together with the bulk impurity screening campaign, the results enabled us to select the radio-purest construction materials, targeting a $^{222}$Rn activity concentration of 10 $\mu$Bq/kg in 3.2 t of xenon. The knowledge of the distribut…

Physics - Instrumentation and DetectorsPhysics and Astronomy (miscellaneous)Radon emanationFOS: Physical scienceschemistry.chemical_element01 natural sciencesNOHigh Energy Physics - Experimentradon: nuclideHigh Energy Physics - Experiment (hep-ex)XENONXenon222 RnPE2_2PE2_10103 physical sciencesActivity concentration[PHYS.HEXP]Physics [physics]/High Energy Physics - Experiment [hep-ex]Dark Matter[PHYS.PHYS.PHYS-INS-DET]Physics [physics]/Physics [physics]/Instrumentation and Detectors [physics.ins-det]010306 general physicsEngineering (miscellaneous)background: radioactivityPhysicsradon: admixture010308 nuclear & particles physicsdetector: surfacescreeningInstrumentation and Detectors (physics.ins-det)chemistryXenon Dark matter 222 Rn radioactivityDark Matter Radon emanation XENON Direct Dark MatterDirect Dark MatterradioactivityAtomic physics
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The effect of dynamical screening on helium (e, 3e) differential cross-sections

2005

A simplified derivation of dynamic screening in the case of three Coulomb-interacting particles in the continuum is given and applied to describe the shapes of multi-differential cross-sections for the (e, 3e) process on the helium atom in which the incident fast electron is treated in first-order Born approximation. A very good agreement with experimental multi-differential cross-sections is obtained for two slow ejected electrons (specifically both electrons have 5 eV) but the strategy appears to be breaking down at intermediate emission energies.

PhysicsDynamic screeningHelium atomchemistry.chemical_elementElectronCondensed Matter PhysicsAtomic and Molecular Physics and Opticschemistry.chemical_compoundchemistryBasso continuoAtomic physicsBorn approximationElectron ionizationHeliumJournal of Physics B: Atomic, Molecular and Optical Physics
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An optimized time screening algorithm for ROSAT PSPC and HRI observations

1998

We have developed a model-independent time screening optimization algorithm to cope with significant contamination spikes in the ROSAT PSPC/HRI bacground light-curves. The rejection criteria are based on the maximization of faint sources signal-to-noise ratio. The algorithm tuning parameters have been optimized through performing a wide set of runs on both simulated and real data. We have verified that the application of our selection criteria to the case of long exposure PSPC observations yields an increase of the number of faint sources ( SNR ) of up to 100% with a rejection of up to the 8% of the exposure time. At the same time, we obtain an average signal-to-noise ratio gain of 3% for t…

PhysicsOptimization algorithmbusiness.industryAstrophysics::High Energy Astrophysical PhenomenaDetectorGeneral Physics and AstronomyAstrophysicsMaximizationScreening algorithmOn boardOpticsROSATInstrumentation (computer programming)businessAlgorithmSelection (genetic algorithm)
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A novel arousal-based individual screening reveals susceptibility and resilience to PTSD-like phenotypes in mice

2021

Translational animal models for studying post-traumatic stress disorder (PTSD) are valuable for elucidating the poorly understood neurobiology of this neuropsychiatric disorder. These models should encompass crucial features, including persistence of PTSD-like phenotypes triggered after exposure to a single traumatic event, trauma susceptibility/resilience and predictive validity. Here we propose a novel arousal-based individual screening (AIS) model that recapitulates all these features. The AIS model was designed by coupling the traumatization (24 h restraint) of C57BL/6 J mice with a novel individual screening. This screening consists of z-normalization of post-trauma changes in startle …

Physiology5-trial SM 5-trial social memoryBiochemistryFight-or-flight responseFST forced swim test0302 clinical medicineEndocrinologySSRIs selective serotonin reuptake inhibitorsDSM-5 Diagnostic and Statistical Manual of Mental DisordersOriginal Research ArticleFear conditioningmedia_commonHT hypothalamusAIS arousal-based individual screeningQP351-495ParoxetinePhenotypeHPA hypothalamic–pituitary–adrenalBST basal synaptic transmissionHIP hippocampusPTSD post-traumatic stress disorder[SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]Psychological resilienceAmy amygdalaRC321-571medicine.drugNeurophysiology and neuropsychologymedia_common.quotation_subjectBDNF brain derived neurotropic factorFear conditioningNeurosciences. Biological psychiatry. NeuropsychiatryBiologyStressArousal03 medical and health sciencesCellular and Molecular NeuroscienceAnimal model Fear conditioning Resilience Stress Susceptibility Z-scoreAnimal modelCORT corticosteroneOF open fieldTE trauma-exposedBiological neural networkmedicineAnimal model[SDV.NEU] Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]C controlfEPSPs field excitatory post-synaptic potentialsSGK1 serum/glucocorticoid-regulated kinase 1RC346-429Molecular BiologyResilienceEndocrine and Autonomic SystemsZ-scoremPFC medial prefrontal cortexFKBP5 FK506 binding protein 5FDA Food and Drug AdministrationASR acoustic startle reactivityEPM elevated plus maze030227 psychiatrySusceptibilityAnimal model; Fear conditioning; Resilience; Stress; Susceptibility; Z-scoreNeurology. Diseases of the nervous systemNeuroscience030217 neurology & neurosurgeryNeurobiology of Stress
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Identification and quantification of meat product ingredients by whole-genome metagenomics (All-Food-Seq)

2019

AbstractComplex food matrices bear the risk of intentional or accidental admixture of non-declared species. Moreover, declared components can be present in false proportions, since expensive taxa might be exchanged for cheaper ones. We have previously reported that PCR-free metagenomic sequencing of total DNA extracted from sausage samples combined with bioinformatic analysis (termed All-Food-Seq, AFS), can be a valuable screening tool to identify the taxon composition of food ingredients. Here we illustrate this principle by analysing regional Doner kebap samples, which revealed unexpected and unlabelled poultry and plant components in three of five cases. In addition, we systematically ap…

Plant Components0303 health sciences030309 nutrition & dietetics04 agricultural and veterinary sciencesGeneral ChemistryComputational biologyBiologyMultiple target040401 food scienceBiochemistryGenomeIndustrial and Manufacturing Engineering03 medical and health sciences0404 agricultural biotechnologyMetagenomicsIdentification (biology)Digital polymerase chain reactionScreening toolFood ScienceBiotechnology
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Effectiveness of screening for known mutations in Sicilian patients with "probable" familial hypercholesterolemia.

2002

Background and Aim: More than 750 mutations in the low-density lipoprotein (LDL) receptor gene are currently known to cause familial hypercholesterolemia (FH), but the array of mutations varies considerably in different populations. The definition of essentially all the LDL receptor gene mutations in a population is therefore a prerequisite for the implementation of nation-wide genetic testing for FH. Methods and Results: In this study, a screening strategy based on PCR-enzymatic digestion and PCR-allele specific hybridisation procedures was used to evaluate the frequency distributions of 11 known mutations in a cohort of 214 unrelated subjects meeting the diagnostic criteria of "probable" …

Point mutationNutrition and DieteticsSettore MED/09 - Medicina InternaEndocrinology Diabetes and MetabolismMedicine (miscellaneous)ExonsPolymerase Chain ReactionFHCohort StudiesHyperlipoproteinemia Type IIGene FrequencyReceptors LDLMutationScreeningHumansGenetic TestingCardiology and Cardiovascular MedicineSicilyFood Science
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