Search results for "script"
showing 10 items of 5143 documents
Interspecies comparison of neuroglobin, cytoglobin and myoglobin: Sequence evolution and candidate regulatory elements
2003
Neuroglobin and cytoglobin are two novel members of the vertebrate globin family. Their physiological role is poorly understood, although both proteins bind oxygen reversibly and may be involved in cellular oxygen homeostasis. Here we investigate the selective constraints on coding and non-coding sequences of the neuroglobin and cytoglobin genes in human, mouse, rat and fish. Neuroglobin and cytoglobin are highly conserved, displaying very low levels of non-synonymous nucleotide substitutions. An oxygen supply function predicts distinct modes of gene regulation, involving hypoxia-responsive transcription factors. To detect conserved candidate regulatory elements, we compared the neuroglobin…
Molecular characterization of the zerknüllt region of the Antennapedia complex of D. subobscura.
1995
We have characterized at the molecular level the zerknullt (zen) region of the Drosophila subobscura Antennapedia complex. The sequence comparison between D. subobscura and D. melanogaster shows an irregular distribution of the conserved and diverged regions, with the homeobox and a putative activating domain completely conserved. Comparisons of the promoter sequence and pattern of expression of the gene during development suggest that the regulation of zen has been conserved during evolution. The conservation of zen expression in a subpopulation of the polar cells indicates the existence of an important role in such cells. We describe a transitory segmented pattern of expression of zen in …
Conserved Structure and Promoter Sequence Similarity in the Mouse and Human Genes Encoding the Zinc Finger Factor BERF-1/BFCOL1/ZBP-89
2001
Abstract We have characterized the genomic structure of the mouse Zfp148 gene encoding Beta-Enolase Repressor Factor-1 (BERF-1), a Kruppel-like zinc finger protein involved in the transcriptional regulation of several genes, which is also termed ZBP-89, BFCOL1. The cloned Zfp148 gene spans 110 kb of genomic DNA encompassing the 5′-end region, 9 exons, 8 introns, and the 3′-untranslated region. The promoter region displays the typical features of a housekeeping gene: a high G+C content and the absence of canonical TATA and CAAT boxes consistent with the multiple transcription initiation sites determined by primary extension analysis. Computer-assisted search in the human genome database allo…
Tīmekļa lietotne sudoku mīklu risināšanai
2019
Sudoku mīklu risināšanas lietotne “Sudoku App” ir tīmeklī bāzēta programmatūra, kas ļauj nereģistrētiem un reģistrētiem lietotājiem risināt sudoku, kā arī, reģistrētiem lietotājiem, saglabāt savu risinājumu vēsturi ar to atrisināšanai patērēto laiku, turpināt jau iepriekš iesāktās mīklas un apskatīt primitīvu statistiku par lietotāja risinātajām sudoku mīklām. Vietnes izstrāde veikta pēc Kanban spējās izstrādes ietvara. Programmatūra izmanto NodeJS tehnoloģiju ar express bibliotēku aizmugursistēmas izstrādē, un ReactJS tehnoloģiju lietotāja saskarnes izstrādē.
Hypertrophic agonists induce the binding of c-Fos to an AP-1 site in cardiac myocytes: implications for the expression of GLUT1
2003
Objectives: Serum is among the agents known to induce hypertrophy of cardiac myocytes, which occurs concomitant with an increase in AP-1-mediated transcription. We have examined if this effect correlates with changes in the relative abundance of particular AP-1 heterodimers, as their exact composition under these conditions is unknown. Furthermore, we obtained insight on the specific role of c-Fos from studying the induction of the glucose transporter GLUT1 by serum in fibroblasts. Methods: We characterised the AP-1 heterodimers expressed in neonatal cardiac myocytes by supershift electrophoretic mobility shift assay (EMSA) analysis. Quantitative changes in transcription were measured using…
FISH mapping of the sex-reversal region on human chromosome 9p in two XY females and in primates
2000
Accumulating evidence suggests that haploinsufficiency of a dosage-sensitive gene(s) in human chromosome 9p24.3 is responsible for the failure of testicular development and feminisation in XY patients with monosomy for 9p. We have used molecular cytogenetic methods to characterise the sex-reversing 9p deletions in two XY females. Fluorescence in situ hybridisation (FISH) with YACs from the critical 9p region containing an evolutionarily conserved sex-determining gene, DMRT1, is a very fast and reliable assay for patient screening. Comparative YAC mapping on great ape and Old and New World monkey chromosomes demonstrated that the critical region was moved from an interstitial position on the…
Moral Responsibility and Moral Harm
2009
I will investigate the relations between the concepts of moral harm and moral responsibility. On such a basis I will question the conceptual soundness of descriptivism, on which consequentialist and non-consequentialist arguments are often grounded. Finally, I will defend a certain version of ascriptivism.
Pluriannual morphological variability in a highly productive Mediterranean reservoir (Lake Arancio, southwestern Sicily)
2007
The progressive decrease in water quality experienced by the Mediterranean, nutrient rich reservoir, Lake Arancio (Southwestern Sicily, Italy), has been accompanied by modifications in both phytoplankton shape and size. Since nutrient concentrations are always far from being limiting, in the present paper attention was focussed on the effects exerted by physical constraints (e.g., mixing, light availability) on the morphological variation observed in phytoplankton. The surface-volume ratio and its product with the maximal linear dimension were chosen as morphological descriptors to trace the trajectories followed by the dominant morphotypes in Lake Arancio. One of these descriptors was comp…
Redescription and phylogenetic analysis of the type species of the genus Panagrellus Thorne, 1938 (Rhabditida, Panagrolaimidae), P. pycnus Thorne, 19…
2021
Abstract The identity of Panagrellus pycnus, the type species of the genus Panagrellus, is discussed after studying specimens from a cultured population collected in Italy that fits the original material of the species. A new characterization is consequently provided as follows: body 0.93–1.32 mm long, lip region continuous with the adjoining body, stoma with gymnostom very reduced, pharynx with not swollen metacorpus, neck 161–203 µm long, excretory pore at level of the metacorpus, post-vulval uterine sac 99–162 µm long or 2.6–3.8 times as long as the body diameter divided in a short tubular proximal part and a long swollen distal part, vulva post-equatorial (V = 63–69), female tail conica…
Architecture of cell differentiation, stress-mediated protein expression and transport in iPSC-derived Motor Neurons bearing a pG376D TDP-43 mutation
2020
BACKGROUND AND RATIONALE. ALS is a fatal neurodegenerative disease caused by loss of motoneurons (MNs). Growingevidence suggests that the disease has a strong genetic basis. Up to now, more than 50 ALS-related genes have beenfound. Among these, TARDBP plays a critical role in ALS. Pathogenic mutations in TARDBP gene are found in 1–3% offamilial ALS cases. A few years ago, a novel G376D TARDBP mutation associated to ALS was reported (Conforti et al, 2011). Recently, a verylarge genealogic tree from this G376D family was made (2HE Association, personal communication) leading to a largecollection of DNA, plasma and fibroblasts (FBs) from several family members, both affected and not clinically…