Search results for "script"
showing 10 items of 5143 documents
Role of Myzus persicae (Hemiptera: Aphididae) and its secondary hosts in plum pox virus propagation
2007
Plum pox virus (family Potyviridae, genus Potyvirus, PPV) is one of the most important viral pathogens of plants in the genus Prunus, particularly Prunus persica L. The role of the Myzus persicae (Sulzer) (Hemiptera: Aphididae) as a vector of PPV-M, and its role in spreading PPV-M, was investigated. PPV-M-infected peach trees were used as inoculum sources, and transmission to 15 herbaceous species commonly present in and around peach orchards was evaluated. The presence of PPV-M in secondary hosts after aphid transmission was verified by reverse transcription-polymerase chain reaction tests. The results indicate that Saponaria ocymoides L., Pisum sativum L., Trifolium repens L., Trifolium p…
Polycomb-like 2 Associates with PRC2 and Regulates Transcriptional Networks during Mouse Embryonic Stem Cell Self-Renewal and Differentiation
2010
SummaryPolycomb group (PcG) proteins are conserved epigenetic transcriptional repressors that control numerous developmental gene expression programs and have recently been implicated in modulating embryonic stem cell (ESC) fate. We identified the PcG protein PCL2 (polycomb-like 2) in a genome-wide screen for regulators of self-renewal and pluripotency and predicted that it would play an important role in mouse ESC-fate determination. Using multiple biochemical strategies, we provide evidence that PCL2 is a Polycomb Repressive Complex 2 (PRC2)-associated protein in mouse ESCs. Knockdown of Pcl2 in ESCs resulted in heightened self-renewal characteristics, defects in differentiation, and alte…
Mouse embryonic stem cells are hypersensitive to apoptosis triggered by the DNA damage O(6)-methylguanine due to high E2F1 regulated mismatch repair.
2007
Exposure of stem cells to genotoxins may lead to embryonic lethality or teratogenic effects. This can be prevented by efficient DNA repair or by eliminating genetically damaged cells. Using undifferentiated mouse embryonic stem (ES) cells as a pluripotent model system, we compared ES cells with differentiated cells, with regard to apoptosis induction by alkylating agents forming the highly mutagenic and killing DNA adduct O(6)-methylguanine. Upon treatment with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), ES cells undergo apoptosis at much higher frequency than differentiated cells, although they express a high level of the repair protein O(6)-methylguanine-DNA methyltransferase (MGMT). Apo…
Gata4 Blocks Somatic Cell Reprogramming By Directly Repressing Nanog
2012
Abstract Somatic cells can be reprogrammed to induced pluripotent stem (iPS) cells by ectopic expression of the four factors Oct4, Klf4, Sox2, and Myc. Here, we investigated the role of Gata4 in the reprogramming process and present evidence for a negative role of this family of transcription factors in the induction of pluripotency. Coexpression of Gata4 with Oct4, Klf4, and Sox2 with or without Myc in mouse embryonic fibroblasts greatly impaired reprogramming and endogenous Nanog expression. The lack of Nanog upregulation was associated with a blockade in the transition from the initiation phase of reprogramming to the full pluripotent state characteristic of iPS cells. Addition of Nanog …
Multiple Pluripotent Stem Cell Markers in Human Anaplastic Thyroid Cancer: The Putative Upstream Role of SOX2
2013
Anaplastic thyroid carcinoma (ATC) is a rare and aggressive endocrine tumor with highly undifferentiated morphology. It has been suggested that cancer stem cells (CSCs) might play a central role in ATC. The objectives of this study were (i) to characterize CSCs from ex vivo ATC specimens by investigating the expression of several pluripotent stem cell markers, and (ii) to evaluate in vitro drug resistance modifications after specific CSC transcription factor switch-off.In ex vivo experiments, eight formalin-fixed, paraffin-embedded ATC specimens were analyzed by reverse-transcription and real-time quantitative PCR and immunohistochemistry. In in vitro experiments using ATC SW1736 cells, the…
Towards an Immersive and Accessible Virtual Reconstruction of Theaters from the Early Modern
2020
This paper reports on the work being done towards achieving an immersive and accessible reconstruction of Historical and Cultural Heritage, focusing on Theaters of the Early Modern as use case. In particular, the paper presents and discusses potential possibilities to enable: 1) the acoustical reconstruction of the virtual (lost) environments – beyond the graphical reconstruction for the buildings, elements and performances; 2) effective interaction features and navigation within the virtual environment (e.g. by means of adaptive interfaces, guiding methods, insertion of Point of Interest); and 3) accessible experiences, by means of an innovative and personalized presentation modes for acce…
Transferir tecnologia para outros espaços escolares
2015
The technology transfer model offers us a perspective to analyze how schools receive incorporate technological activities and their equipment. Particularly, we analyze the innovations generated by these processes at the organizational level of schools as recipients of technology. The analyzed data belong to an investigation which is currently being performed in 12 regions on the “School 2.0 program”. At this stage we handle references of descriptive statistics and some of the interviews and classroom observations from the participating centers on the “smart school”. From the first analysis, it can be said that the school system follows a pattern transfer including: high dependence on the po…
Political Scientist Reads Gramsci: From Hegemony to teh Political
2015
In this paper I want to show, the Antonio Gramsci’s arrangements, Italian Marxist, are very actual analysis of political process and phenomenon, which can be very significant tip and inspiration for the political researchers. The argument of this paper is about a kind of continuity between process of establishing hegemony and notice what the political (beyond the strictly policy). Analyzing this continuity, I want to do some kind of “political science transcription” of Gramsci, which I hope will result in more detailed research. Also I want to show the Gramsci’s profile as a political theoretist.
Transcriptomic, biochemical and individual markers in transplanted Daphnia magna to characterize impacts in the field
2015
Daphnia magna individuals were transplanted across 12 sites from three Spanish river basins (Llobregat, Ebro, Jucar) showing different sources of pollution. Gene transcription, feeding and biochemical responses in the field were assessed and compared with those obtained in re-constituted water treatments spiked with organic eluates obtained from water samples collected at the same locations and sampling periods. Up to 166 trace contaminants were detected in water and classified by their mode of action into 45 groups that included metals, pharmaceuticals, pesticides, illicit drugs, and other industrial compounds. Physicochemical water parameters differentiated the three river basins with Llo…
Impact of Industrial SMEs in the Environment Conservation : A Systematic Mapping Study
2020
Small and medium-sized companies have an impact on the environment where they operate. In some cases, the effects on the environment can be significant within local communities leading to a series of negative processes, such as emission pollution into the atmosphere, the discharges to rivers and seas, the production of waste; in some cases, it makes the contamination to the soil. This paper presents the results of the systematic mapping study aimed at assessing environmental management in industrial SMEs. Descriptive studies of SMEs emphasize their poor environmental commitment, describing them as primarily interested in controlling pollutant emissions just to comply with regulations. Ninet…