Search results for "script"
showing 10 items of 5143 documents
Inhibition of in vitro reconstitution of rotavirus transcriptionally active particles by anti-VP6 monoclonal antibodies
1994
International audience; Six monoclonal antibodies specific for the major capsid protein of rotavirus, VP6, previously characterized, were tested in a biological assay for their capacity to block the transcriptase activity associated with the single-shelled particles. The results showed that two MAbs (RV-50 and RV-133), specific for distinct antigenic sites, were able to block the transcription when they were incubated with a purified baculovirus-expressed group A VP6, prior to the reconstitution of the single-shelled particles from the cores, suggesting that at least two domains are involved in active single-shelled particle reconstitution. The results obtained previously from immunochemist…
Unexpected substitution of dominant rotavirus G genotypes in French hospitalized children over five consecutive seasons.
2009
International audience; The study was designed to evaluate the circulation of group A rotaviruses in French hospitalized children, and to detect unusual strains. This prospective study was conducted from 2001 to 2006 in children consulting for acute diarrhea at the pediatric emergency department in three French University Hospitals. The rotaviruses were detected by rapid test and genotyped by RT-PCR on the basis of their outer capsid proteins VP4 (P-type) and VP7 (G-type). The stools from 757 children were analyzed. G1P[8] strains were predominant (44.0%), followed by G9P[8] (17.7%), G3P[8] 13.1%, G4P[8] (9.5%), and G2P[4] (1.8%); mixed rotavirus infections occurred in 2.3%. G9 rotaviruses …
In vitro reconstitution of rotavirus transcriptional activity using viral cores and recombinant baculovirus expressed VP 6
1993
International audience; Purified baculovirus-expressed group A rotavirus VP6 polypeptide was shown to be active in the recovery of the transcriptase activity associated with the reconstitution of the single-shelled rotavirus particle. Recombinant VP6 polypeptide was able to restore the transcriptional activity in purified viral cores from both SA-11 and RF rotavirus strains. Recombinant group C VP 6 (Cowden strain) is capable of binding as a trimer to group A viral core particles but unable to restore the transcriptase activity, suggesting that the binding of the polypeptide to cores is not the only requirement to restore the transcriptase activity. The VP 6 group A polypeptide was shown to…
La povertà rurale in Africa Sub-sahariana: il caso del Dipartimento della Lekie in Camerun
2021
Questo articolo mette in rilievo le caratteristiche della povertà rurale in Africa sub-sahariana in generale, e nel Dipartimento della Lekie in Camerun in particolare. Il suo interesse sta nel fatto che per meglio combattere la povertà, bisogna innanzitutto capire la sua vera natura e le sue particolari caratteristiche sul territorio d’intervento. Questo studio mira dunque a offrire alla comunità scientifica nonché a vari portatori d’interesse un quadro di riferimento per la definizione di politiche economiche efficaci contro la povertà sul territorio del Dipartimento della Lekie. L’indagine svolta sul cosiddetto territorio ha rilevato, tramite un’analisi descrittiva, che le popolazioni del…
The telomeric Cdc13-Stn1-Ten1 complex regulates RNA polymerase II transcription
2019
Advance article.
Moscato Cerletti, a rediscovered aromatic cultivar with oenological potential in warm and dry areas
2021
Baron Antonio Mendola was devoted to the study of grapevine, applying ampelography and dabbling in crosses between cultivars in order to select new ones, of which Moscato Cerletti, obtained in 1869, was the most interesting. Grillo, one of the most important white cultivars in Sicily, was ascertained to be an offspring of Catarratto Comune and Zibibbo, the same parents which Mendola claimed he used to obtain Moscato Cerletti. Thus the hypothesis of synonymy between Moscato Cerletti and Grillo or the same parentage for both sets of parents needs to be verified.In the present study, historical documents were consulted and genetic analyses and ampelographic, agronomic and qualitative character…
(AUTO-)ETHNOGRAPHIC SELVES, MASCULINITIES AND RACE IN MALE SEX WORK ARENAS
2014
THE PAPER EXPLORES THE WAYS MALE SEX WORKERS NEGOTIATE AND REDEFINE THEIR GENDER AND THEIR SEXUAL ROLES WHEN INVOLVED IN NON-NORMATIVE SEXUAL PRACTICES.
Coptis chinensis Franch. exhibits neuroprotective properties against oxidative stress in human neuroblastoma cells.
2014
Abstract Ethnopharmacological relevance The dried rhizome of Coptis chinensis Franch. (family Ranunculaceae ) is traditionally used in Chinese medicine for the treatment of inflammatory diseases and diabetes. Recent studies showed a variety of activities of Coptis chinensis Franch. alkaloids, including neuroprotective, neuroregenerative, anti-diabetic, anti-oxidative and anti-inflammatory effects. However, there is no report on the neuroprotective effect of Coptis chinensis Franch. watery extract against tert -butylhydroperoxide ( t -BOOH) induced oxidative damage. The aim of the study is to investigate neuroprotective properties of Coptis chinensis Franch. rhizome watery extract (CRE) and …
Polymorphisms of beta-lactoglobulin promoter region in three Sicilian goat breeds
2012
Several beta-lactoglobulin (BLG) polymorphisms have been described within the proximal promoter region and coding region of the caprine gene, although no genetic variants affecting the protein amino acid composition and/or expression level have been characterized so far. Binding sites for several transcription factors (TFs) are present in the BLG promoter region. The aims of this work were to sequence the full-length promoter region of three Sicilian goat breeds in order to identify polymorphisms, analyze the identified haplotypes, search for differences between breeds for the presence of polymorphisms in this gene region, search for putative TFs binding sites, and check if polymorphisms la…
RDB2OWL
2010
RDB2OWL is a simple approach of mapping relational databases into independently developed OWL ontologies. The approach is based on creating a mapping RDB schema, filling it with mapping information from which SQL scripts are generated that perform the instance-level transformation. We describe the RDB2OWL mapping schema and report on successful application of the technology to the migration of Latvian medical registries data.