Search results for "seldi-tof-ms"

showing 2 items of 2 documents

Saliva analysis by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) in orthodontic treatment: first pilot…

2011

Abstract Objective SELDI-TOF-MS (Surface-Enhanced Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry) allows the generation of an accurate protein profile from minimal amounts of biological samples and may executes proteomic profile of saliva. The aim of this work is to compare the proteomic profile of saliva of patients in orthodontic treatment to the beginning of treatment and after three months by using the surface enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) technology. Materials and methods Saliva was collected from 14 patients, between the 11 and 17 years, to the beginning of the orthodontic treatment and after three months. Specimens …

MaleSaliva analysis SELDI-TOF-MS orthodontic treatmentSalivaAdolescentProteomePilot ProjectsOrthodonticsSinapinic acidMass spectrometrySensitivity and SpecificityOrthodontics CorrectiveMatrix (chemical analysis)chemistry.chemical_compoundOrthodontic AppliancesSELDI-TOF-MSHumansSalivary Proteins and PeptidesChildSalivaProteomic ProfileChromatographytooth movement; orthodontic treatment; seldi-tof-mstooth movementSurface-enhanced laser desorption/ionizationchemistrySpectrometry Mass Matrix-Assisted Laser Desorption-IonizationFemaleorthodontic treatmentTime-of-flight mass spectrometryseldi-tof-ms
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SELDI-TOF-MS ProteinChip array profiling of tears from patients with dry eye.

2005

Protein and peptides in tears play an important role in ocular surface diseases. In previous studies, changes have been demonstrated in the electrophoretic protein profiles of patients with dry eye. The purpose of this work was to determine the usefulness of surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) ProteinChip Array (Ciphergen Biosystems, Inc., Fremont, CA) technology for the automated analysis of proteins and peptides in tear fluid.Patients with dry eye (DRY, n = 88) and healthy subjects (CTRL, n = 71) were examined. Their tear proteins were analyzed using SELDI-TOF-MS ProteinChip Arrays with three different chromatographic surfaces (CM10…

Pathologymedicine.medical_specialtyEye diseaseProtein Array AnalysisDry Eye SyndromesLipocalinchemistry.chemical_compoundSELDI-TOF-MSmedicineHumansIn patientEye ProteinsChromatography High Pressure LiquidChromatographybusiness.industryHealthy subjectsmedicine.diseaseChromatography Ion ExchangechemistrySpectrometry Mass Matrix-Assisted Laser Desorption-IonizationTearsTearsDry Eye SyndromesNeural Networks ComputerLysozymebusinessPeptidesBiomarkersInvestigative ophthalmologyvisual science
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