Search results for "sequence"
showing 10 items of 4987 documents
Changes in carbohydrate metabolism in Plasmopara viticola-infected grapevine leaves.
2011
International audience; The oomycete Plasmopara viticola is responsible for downy mildew, a severe grapevine disease. In infected grapevine leaves, we have observed an abnormal starch accumulation at the end of the dark period, suggesting modifications in starch metabolism. Therefore, several complementary approaches, including transcriptomic analyses, measurements of enzyme activities, and sugar quantification, were performed in order to investigate and to understand the effects of P. viticola infection on leaf starch and-to a larger extent-carbohydrate metabolism. Our results indicate that starch accumulation is associated with an increase in ADP-glucose pyrophosphorylase (AGPase) activit…
Succession in ant communities (Hymenoptera: Formicidae) in deciduous forest clear-cuts - an Eastern European case study
2017
Clear-cutting, the main method of harvesting in many forests in the world, causes a series of dramatic environmental changes to the forest habitat and removes habitat resources for arboreal and epigeal species. It results in considerable changes in the composition of both plant and animal communities. Ants have many critical roles in the maintenance and functioning of forest ecosystems. Therefore, the response of ants to clear-cutting and the time it takes for an ant community to recover after clear-cutting are important indicators of the effect of this harvesting technique on the forest ecosystem. We investigated ground-dwelling ant communities during secondary succession of deciduous fore…
The genome sequencing of an albino Western lowland gorilla reveals inbreeding in the wild
2013
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.-- et al.
Pleurotus opuntiae revisited e An insight to the phylogeny of dimitic Pleurotus species with emphasis on the P. djamor complex
2018
Abstract The name Pleurotus opuntiae is indiscriminately used for describing mushrooms with white to off-white to white-grey pilei with short or absent stipe and dimitic hyphal system, which grow on plants of the genera Opuntia, Yucca, Agave, Phytolacca etc. However, the outcome of the present study evidences that this name should be reserved for specimens deriving from the Mediterranean area only; an epitype originating from Italy on Opuntia ficus-indica is designated. Pertinent material was sequenced by using the internal transcribed spacer region (ITS) and found to be phylogenetically related to P. djamor from Kenya and Nigeria, while members of the P. djamor complex from other continent…
AtCCS is a functional homolog of the yeast copper chaperone Ccs1/Lys7
2005
AbstractIn plant chloroplasts two superoxide dismutase (SOD) activities occur, FeSOD and Cu/ZnSOD, with reciprocal regulation in response to copper availability. This system presents a unique model to study the regulation of metal-cofactor delivery to an organelle. The Arabidopsis thaliana gene AtCCS encodes a functional homolog to yeast Ccs1p/Lys7p, a copper chaperone for SOD. The AtCCS protein was localized to chloroplasts where it may supply copper to the stromal Cu/ZnSOD. AtCCS mRNA expression levels are upregulated in response to Cu-feeding and senescence. We propose that AtCCS expression is regulated to allow the most optimal use of Cu for photosynthesis.
Subcellular localization and purification of a p-hydroxyphenylpyruvate dioxygenase from cultured carrot cells and characterization of the correspondi…
1997
p-Hydroxyphenylpyruvate dioxygenase catalyses the transformation of p-hydroxyphenylpyruvate into homogentisate. In plants this enzyme has a crucial role because homogentisate is the aromatic precursor of all prenylquinones. Furthermore this enzyme was recently identified as the molecular target for new families of potent herbicides. In this study we examine precisely the localization of p-hydroxyphenylpyruvate dioxygenase activity within carrot cells. Our results provide evidence that, in cultured carrot cells, p-hydroxyphenylpyruvate dioxygenase is associated with the cytosol. Purification and SDS/PAGE analysis of this enzyme revealed that its activity is associated with a polypeptide of 4…
The dominance of the herbicide resistance cost in several Arabidopsis thaliana mutant lines
2004
Abstract Resistance evolution depends upon the balance between advantage and disadvantage (cost) conferred in treated and untreated areas. By analyzing morphological characters and simple fitness components, the cost associated with each of eight herbicide resistance alleles (acetolactate synthase, cellulose synthase, and auxin-induced target genes) was studied in the model plant Arabidopsis thaliana. The use of allele-specific PCR to discriminate between heterozygous and homozygous plants was used to provide insights into the dominance of the resistance cost, a parameter rarely described. Morphological characters appear more sensitive than fitness (seed production) because 6 vs. 4 differen…
Constitutive Activation of the Midgut Response to Bacillus thuringiensis in Bt-Resistant Spodoptera exigua
2010
Bacillus thuringiensis is the most effective microbial control agent for controlling numerous species from different insect orders. The main threat for the long term use of B. thuringiensis in pest control is the ability of insects to develop resistance. Thus, the identification of insect genes involved in conferring resistance is of paramount importance. A colony of Spodoptera exigua (Lepidoptera: Noctuidae) was selected for 15 years in the laboratory for resistance to Xentari (TM), a B. thuringiensis-based insecticide, reaching a final resistance level of greater than 1,000-fold. Around 600 midgut ESTs were analyzed by DNA-macroarray in order to find differences in midgut gene expression …
Mitochondrial phylogeography of the edible dormouse (Glis glis) in the western Palearctic region
2010
International audience; This study describes in detail the phylogeoraphic pattern Of the edible dormouse (Glis glis) a European rodent With pronounced hibernating behavior We Used sequences of 831 base pairs of the mitochondrial DNA cytochrome-b gene from 130 edible dormice collected at 43 localities (throughout Its distribution. Our results reveal presence of 3 main haplogroups: Sicilian, South Italian (restricted to the Calabrian region) (a widespread lineage corresponding to all remaining western, central. and eastern European populations). Examination of paleontological data confirms refugial regions for G,Its in the 3 Mediterranean peninsulas, although overall low genetic diversity is …
Bryophyte Species Richness on Retention Aspens Recovers in Time but Community Structure Does Not
2014
Green-tree retention is a forest management method in which some living trees are left on a logged area. The aim is to offer ‘lifeboats’ to support species immediately after logging and to provide microhabitats during and after forest re-establishment. Several studies have shown immediate decline in bryophyte diversity after retention logging and thus questioned the effectiveness of this method, but longer term studies are lacking. Here we studied the epiphytic bryophytes on European aspen (Populus tremula L.) retention trees along a 30-year chronosequence. We compared the bryophyte flora of 102 ‘retention aspens’ on 14 differently aged retention sites with 102 ‘conservation aspens’ on 14 d…