Search results for "sequence"
showing 10 items of 4987 documents
Functional characterization of the human tRNA methyltransferases TRMT10A and TRMT10B
2020
Abstract The TRM10 family of methyltransferases is responsible for the N1-methylation of purines at position 9 of tRNAs in Archaea and Eukarya. The human genome encodes three TRM10-type enzymes, of which only the mitochondrial TRMT10C was previously characterized in detail, whereas the functional significance of the two presumably nuclear enzymes TRMT10A and TRMT10B remained unexplained. Here we show that TRMT10A is m1G9-specific and methylates a subset of nuclear-encoded tRNAs, whilst TRMT10B is the first m1A9-specific tRNA methyltransferase found in eukaryotes and is responsible for the modification of a single nuclear-encoded tRNA. Furthermore, we show that the lack of G9 methylation cau…
Two-phase routing in three-dimensional blocked optical tori
2014
The contribution of this paper is an all-optical 3D network architecture. We describe scheduled, two-phase routing for it. The three-dimensional blocked optical torus BOT of block size b consists of b2 × b2 × b2 nodes for the first phase routing. Processors are evenly deployed at the underlying torus so that every bth node consists of a processor. Additionally, a BOT consists of b3 blocks of b × b × b subnetworks for the second phase routing. Routing of each packet is done in two phases. Firstly, packets are routed from source processor to an intermediate target node at the target block. Secondly, packets are routed from the intermediate targets at the target block to the target processor (…
H.264 QoS and Application Performance with Different Streaming Protocols
2015
Streaming techniques, including the selected streaming protocol, have an effect on the streaming quality. In this study, the performance of three different streaming protocols in a disturbed communication channel is evaluated with a modified version of the FFPlay player. A H.264 encoded video is used as a test sequence. The number of displayed image frames, the frame rate and playout duration are used as objective metrics for QoS. The metrics brings out differences of streaming protocols in our test environment. They are measured at the application level and have a connection to the user experience. peerReviewed
Real-time recognition of personal routes using instance-based learning
2011
Predicting routes is a critical enabler for many new location-based applications and services, such as warning drivers about congestion- or accident-risky areas. Hybrid vehicles can also utilize the route prediction for optimizing their charging and discharging phases. In this paper, a new lightweight route recognition approach using instance-based learning is introduced. In this approach, the current route is compared in real-time against the route instances observed in past, and the most similar route is selected. In order to assess the similarity between the routes, a similarity measure based on the longest common subsequence (LCSS) is employed, and an algorithm for incrementally evaluat…
Repetitive sequence distribution on Saguinus, Leontocebus and Leontopithecus tamarins (Platyrrhine, Primates) by mapping telomeric (TTAGGG) motifs an…
2021
Simple Summary Telomeric and rDNA sequence distribution on tamarins (New world monkeys, Primates) was analysed through molecular cytogenetics by fluorescence in situ hybridization. The mapping of Telomeric and rDNA probes on chromosomes was performed in order to clarify their localization and role in genome evolution. We found rDNA loci on the same homologs 19–22 on the analysed species with a different position in one of them named Leontopithecus rosalia, presumably as result of inversions. Other rDNA signals could be present on chromosome 16 and 17. On the last species, we found the classic telomeric sequence with exceptions while on the other species analysed, we found very amplified tel…
Distinctive attributes for predicted secondary structures at terminal sequences of non-classically secreted proteins from proteobacteria
2008
Abstract C- and N-terminal sequences (64 amino acid residues each) of 89 non-classically secreted type I, type III and type IV proteins (Swiss-Prot/TrEMBL) from proteobacteria were transformed into predicted secondary structures. Multivariate analysis of variance (MANOVA) confirmed the significance of location (C- or N-termini) and secretion type as essential factors in respect of quantitative representations of structured (a-helices, b-strands) and unstructured (coils) elements. The profiles of secondary structures were transcripted using unequal property values for helices, strands and coils and corresponding numerical vectors (independent variables) were subjected to multiple discriminan…
An extended catalogue of ncRNAs in Streptomyces coelicolor reporting abundant tmRNA, RNase-P RNA and RNA fragments derived from pre-ribosomal RNA lea…
2022
Streptomyces coelicolor is a model organism for studying streptomycetes. This genus possesses relevant medical and economical roles, because it produces many biologically active metabolites of pharmaceutical interest, including the majority of commercialized antibiotics. In this bioinformatic study, the transcriptome of S. coelicolor has been analyzed to identify novel RNA species and quantify the expression of both annotated and novel transcripts in solid and liquid growth medium cultures at different times. The major characteristics disclosed in this study are: (i) the diffuse antisense transcription; (ii) the great abundance of transfer-messenger RNAs (tmRNA); (iii) the abundance of rnpB…
Evolutionary advantage conferred by an eukaryote-to-eukaryote gene transfer event in wine yeasts
2015
Although an increasing number of horizontal gene transfers have been reported in eukaryotes, experimental evidence for their adaptive value is lacking. Here, we report the recent transfer of a 158-kb genomic region between Torulaspora microellipsoides and Saccharomyces cerevisiae wine yeasts or closely related strains. This genomic region has undergone several rearrangements in S. cerevisiae strains, including gene loss and gene conversion between two tandemly duplicated FOT genes encoding oligopeptide transporters. We show that FOT genes confer a strong competitive advantage during grape must fermentation by increasing the number and diversity of oligopeptides that yeast can utilize as a s…
Double copies of blaKPC-3::Tn4401a on an IncX3 plasmid in Klebsiella pneumoniae successful clone ST512 from Italy
2015
ABSTRACT A carbapenem-resistant sequence type 512 (ST512) Klebsiella pneumoniae carbapenemase 3 (KPC-3)-producing K. pneumoniae strain showing a novel variant plasmid content was isolated in Palermo, Italy, in 2014. ST512 is a worldwide successful clone associated with the spread of bla KPC genes located on the IncFIIk pKpQIL plasmid. In our ST512 strain, the bla KPC-3 gene was unusually located on an IncX3 plasmid, whose complete sequence was determined. Two copies of bla KPC-3 ::Tn 4401a caused by intramolecular transposition events were detected in the plasmid.
Identification of mycorrhiza-regulated genes with arbuscule development-related expression profile
2004
Suppressive subtractive hybridisation was applied to the analysis of late stage arbuscular mycorrhizal development in pea. 96 cDNA clones were amplified and 81, which carried fragments more than 200 nt in size, were sequence analysed. Among 67 unique fragments, 10 showed no homology and 10 were similar to sequences with unknown function. RNA accumulation of the corresponding 67 genes was analysed by hybridisation of macro-arrays. The cDNAs used as probes were derived from roots of wild type and late mutant pea genotypes, inoculated or not with the AM fungus Glomus mosseae. After calibration, a more than 2.5-fold mycorrhiza-induced RNA accumulation was detected in two independent experiments…