Search results for "sequencing"

Dynamique de la communauté microbienne de sols tropicaux suite à une application répétée de fertilisants organiques et minéraux

2019

Organic waste products (OWP) with NPK mineral fertilization are known to have an impact on soil microbial communities. However, dynamic of these communities following a repeated OWP and NPK application has not been well described. In the present study, this dynamic was observed on soil sampled at the La Mare (La Réunion) field experiment, which was set up in 2013. This experiment includes control plot (without organic input, but mineral fertilization) and several plots receiving different types of OWP coupled with NPK fertilization. Physico-chemical parameters were measured on soils and OWPs (2013 to 2017). Soil microbial biomass (from 2013 to 2018) and the structure of microbial community …

Consistency of Targeted Metatranscriptomics and Morphological Characterization of Phytoplankton Communities

2020

The composition of phytoplankton community is the basis for environmental monitoring and assessment of the ecological status of aquatic ecosystems. Community composition studies of phytoplankton have been based on time-consuming and expertise-demanding light microscopy analyses. Molecular methods have the potential to replace microscopy, but the high copy number variation of ribosomal genes and the lack of universal primers for simultaneous amplification of prokaryotic and eukaryotic genes complicate data interpretation. In this study, we used our previously developed directional primer-independent high-throughput sequencing (HTS) approach to analyze 16S and 18S rRNA community structures. C…

Capacity building in clinical and molecular characterization of rare neurodegenerative diseases through the development of a local register and impro…

2020

Neurodegenerative diseases (NDs) are very polymorphic and affect people of all ages. They concern both rare diseases and more common diseases. Improving knowledge of NDs in the French Caribbean region requires special attention because of the unique environmental and genetic background of its populations. In Martinique, there is a huge need for scientific research on rare NDs. While epidemiological and clinical research has increased over the past decade in the French West Indies, there is still little or no data on the genetics of NDs in these regions. The advent of Next Generation Sequencing (NGS) on a global scale has the potential to make a difference in this field, provided that techno…

Possible Editing of Alu Transcripts in Blood Cells of Sporadic Creutzfeldt–Jakob Disease (sCJD)

2011

Editing of RNA molecules gained major interest when coding mRNA was analyzed. A small, noncoding, Alu DNA element transcript that may act as regulatory RNA in cells was examined in this study. Alu DNA element transcription was determined in buffy coat from healthy humans and human sporadic Creutzfeldt-Jakob disease (sCJD) cases. In addition, non-sCJD controls, mostly dementia cases and Alzheimer's disease (AD) cases, were included. The Alu cDNA sequences were aligned to genomic Alu DNA elements by database search. A comparison of best aligned Alu DNA sequences with our RNA/cDNA clones revealed editing by deamination by ADAR (adenosine deaminase acting on RNA) and APOBEC (apolipoprotein B ed…

Removal of bis (2-ethylhexyl) phthalate from reject water in a nitrogen-removing sequencing batch reactor.

2003

Reject water from sewage sludge processing may contain high concentrations of nutrients and organic pollutants and cause internal pollution load at a sewage treatment plant (STP) if circulated to the headworks of an STP. In the present study removal of nitrogen and bis (2-ethylhexyl) phthalate (DEHP) from reject water was studied in two sequencing batch reactors (SBRs) with different aerobic/anoxic periods during a 6-h total cycle period. Ammonia-nitrogen (NH(4)-N) was almost totally removed in both reactors, apparently by nitrification throughout the run, while denitrification declined with decreasing SCOD in the influent resulting in an increase in the effluent nitrate-nitrogen (NO(3)-N) …

Exercise-Induced Extracellular Vesicles Delay the Progression of Prostate Cancer

2022

Increasing evidence suggests that regular physical exercise not only reduces the risk of cancer but also improves functional capacity, treatment efficacy and disease outcome in cancer patients. At least partially, these effects are mediated by the secretome of the tissues responding to exercise. The secreted molecules can be released in a carrier-free form or enclosed into extracellular vesicles (EVs). Several recent studies have shown that EVs are actively released into circulation during physical exercise. Here, we for the first time investigated the effects of exercise-induced EVs on the progression of cancer in an F344 rat model of metastatic prostate cancer. Although we did not observe…

Molecular and cytological characterization of homozygous plants of Citrus clementina Hort. ex Tan., candidates to citrus genome sequencing. .

2010

Differential Contribution of the Parental Genomes to a

2019

In European regions of cold climate, S. uvarum can replace S. cerevisiae in wine fermentations performed at low temperatures. S. uvarum is a cryotolerant yeast that produces more glycerol, less acetic acid and exhibits a better aroma profile. However, this species exhibits a poor ethanol tolerance compared with S. cerevisiae. In the present study, we obtained by rare mating (non-GMO strategy), and a subsequent sporulation, an interspecific S. cerevisiae × S. uvarum spore-derivative hybrid that improves or maintains a combination of parental traits of interest for the wine industry, such as good fermentation performance, increased ethanol tolerance, and high glycerol and aroma productions. G…

Effect of long-term salinity increase on nitritation and denitritation kinetics in activated sludge and granular sludge reactors

2017

The aim of this work was to investigate the effects of moderate and drastic long-termincrease of salinity.Particularly, the study was aimed at assessing the shock effecton nitritationand denitritation kineticsof halophilic biomass inboth forms:flocculent activated sludge and granular sludge.

Short-term impact of biogas digestates on soils microbial communities

2022

International audience; Anaerobic digestion of organic waste is considered a key process to produce renewable energy to meet the growing sustainable energy demand. Digestates can be used in agriculture as soil amendments and improve crop yields. However, their use at large scale in agricultural fields still requires to prove their innocuity on soil biota, especially on microorganisms that play important roles in soil ecosystem. Here, we designed a microcosm experiment to compare the short-term (42-days) effects of four different digestates (derived from cattle manure, energy crop, food residues or slurry with bio-waste) on the soil microbial communities. Each digestate was applied on three …