Search results for "serotype"
showing 10 items of 130 documents
Isolation of a new serovar of Vibrio vulnificus pathogenic for eels cultured in freshwater farms
2003
The present work reports the isolation of a new serovar of Vibrio vulnificus pathogenic for eels cultured in freshwater farms. This serovar, named Serovar A, produced two outbreaks of low morbidity and mortality in one Spanish eel farm in September 2000 and March 2001. The vibriosis affected healthy eels, weighting between 10 and 100 g, that had been vaccinated against the serovar E of the species (formerly biotype 2) at least 6 months before. Some of the diseased eels showed symptoms different to those reported for the classical vibriosis due to serovar E. These symptoms included bleeding ventral ulcers together with haemorrhagic intestine. Bath experimental challenges demonstrated that (i…
Molecular Typing Reveals Frequent Clustering among Human Isolates of Listeria monocytogenes in Italy
2009
In Italy, the annual incidence of reported cases of listeriosis amounts in recent years (2004 to 2006) to 0.8 cases per million inhabitants. Our study is a subtyping analysis by serotyping, ribotyping, and pulsed-field gel electrophoresis analysis of 44 human isolates from apparently sporadic cases of infection in the Lombardy region and in the Province of Florence, Italy, in the years 1996 to 2007. Based on the results of the different subtyping methods, 10 occasions were detected when strains of L. monocytogenes with the same subtype were isolated from more than one listeriosis case. A total of 28 (66.7%) out of 44 isolates were attributed to molecular subtype clusters. Our data support t…
Immune Response to the 23-Valent Polysaccharide Pneumococcal Vaccine (PPV23) after the 7-Valent Conjugate Vaccine (PCV7) in Allogeneic Stem Cell Tran…
2008
Abstract Background: Pneumococcal infections are causes of death after SCT. The IDWP01 trial compared early (E) vs late (L) pneumococcal immunization after allogeneic SCT, starting either at 3, or at 9 months post-transplant with 3 doses of PCV7 given at 1 month intervals,. This study has shown that the early immunization was not inferior in the proportion of responders one month after the last PCV7 dose. In addition, all patients received one dose of PPV23 after the 3 doses of PCV7 at 12 months and 18 months in the E and L groups, respectively. The goal of this supplementary analysis was to look at the immune response to the PPV23 according to the time of its administration. Methods: 158 p…
Infections caused by Pseudomonas aeruginosa: relatively frequent isolation of serogroup 12 from clinical specimens.
1985
Serological typing of P. aeruginosa is the most simple and reliable procedure recommended for ≪ in-house ≫ investigations and for studies of suspected outbreaks of infection by this microorganism. It is also a useful procedure in order to know serotype prevalence in a definite geografical area and to obtain indications about the more appropriate composition of polivalent anti-Pseudomonas vaccines. In the present report, we describe the relatively high frequency of isolation of serogroup 12 from patients in Palermo, Italy. Serogroup 12 is very rare in north-Europe and in the USA, and, as a consequence, it is not included in some vaccine preparations. In Palermo, strains belonging to this ser…
Electropherotype heterogeneity within serotypes of human rotavirus strains circulating in Italy
1987
Using solid-phase immune electron microscopy, 126 of 129 human rotavirus (HRV) strains could be serotyped directly in stools collected in Italy during the period 1981–1985. Prevalence was 70.5 per cent for serotype 1, and 13.2 per cent for each of serotypes 2 and 4. No serotype 3 strain was detected. In parallel, for 39 of 61 HRV strains tested the electropherotype of genomic RNA was successfully determined. Different electropherotypes were detected among strains of the same serotype, whereas the same electropherotype was found in HRV strains of different serotypes. Serotyping and electropherotyping of HRV strains appear to be complementary to each other, and both should be used in conjunct…
Characterization of the first extended-spectrum beta-lactamase-producing nontyphoidal Salmonella strains isolated in Tehran, Iran.
2009
The infections caused by Salmonella remain a significant public health problem throughout the world. beta-Lactams and fluoroquinolones are generally used to treat invasive Salmonella infections, but emergence and spread of antibiotic-resistant strains are being increasingly notified in many countries. In particular, detection of extended-spectrum beta-lactamases (ESBLs) in Salmonella spp. is a newly emerging threat worldwide. This study was carried out to characterize beta-lactamase-producing Salmonella strains identified in Tehran, Iran. Over the 2-year period from 2007 to 2008, 6 of 136 Salmonella isolates recovered from pediatrics patients, including three Salmonella enterica serotypes E…
Epidemic dissemination of Salmonella enterica spp. enterica serovar Bovismorbificans in southern Italy in the years 1989-1991.
1994
Epidemic strains of Salmonella enterica subsp. enterica serovar Bovismorbificans isolated in southern Italy during the years 1989-1991 were submitted to a molecular epidemiological study in comparison with isolates identified in the years 1980-1988 in the same geographic area. Genomic DNA fragments obtained by digestion with BglI or Eco RI hybridized with Escherichia coli rRNA to produce three distinct, but highly related patterns. Ribotype 1, which had never been identified before 1989, was found to characterize most of the strains identified between 1989 and 1991. Such a finding supports the hypothesis of emergence and spread of a new bacterial clone associated with the increased number o…
Analysis of Multilocus Sequence Typing and Virulence Characterization of Listeria monocytogenes Isolates from Chinese Retail Ready-to-Eat Food
2016
Eighty Listeria monocytogenes isolates were obtained from Chinese retail ready-to-eat (RTE) food and were previously characterized with serotyping and antibiotic susceptibility tests. The aim of this study was to characterize the subtype and virulence potential of these L. monocytogenes isolates by multilocus sequence typing (MLST), virulence-associate genes, epidemic clones (ECs), and sequence analysis of the important virulence factor: internalin A (inlA). The result of MLST revealed that these L. monocytogenes isolates belonged to 14 different sequence types (STs). With the exception of four new STs (ST804, ST805, ST806, and ST807), all other STs observed in this study have been associat…
TLR7 controls VSV replication in CD169(+) SCS macrophages and associated viral neuroinvasion
2019
Vesicular stomatitis virus (VSV) is an insect-transmitted rhabdovirus that is neurovirulent in mice. Upon peripheral VSV infection, CD169+ subcapsular sinus (SCS) macrophages capture VSV in the lymph, support viral replication, and prevent CNS neuroinvasion. To date, the precise mechanisms controlling VSV infection in SCS macrophages remain incompletely understood. Here, we show that Toll-like receptor-7 (TLR7), the main sensing receptor for VSV, is central in controlling lymph-borne VSV infection. Following VSV skin infection, TLR7−/− mice display significantly less VSV titers in the draining lymph nodes (dLN) and viral replication is attenuated in SCS macrophages. In contrast to effects o…
Serological and molecular identification of Legionella spp. isolated from water and surrounding air samples in Italian healthcare facilities
2016
Abstract Background Legionella is an intracellular microorganism living in natural and artificial aquatic environments. Although its transmission to humans is linked to the inhalation of contaminated aerosols, there is no validated air sampling method for the control and prevention of the disease. The aim of the present study was to provide more information on the distribution of Legionella spp. in indoor environments and to determine whether the same Legionella strains are isolated from air and water samples. Methods Ten healthcare facilities located in seven regions of Italy were enrolled. The serological typing of Legionella spp. from water samples and the surrounding air by active and p…