Search results for "serp"

Construction and expression of a dual vector for chemo-enzymatic synthesis of plant indole alkaloids inEscherichia coli

2010

A dual vector (pQE-70-STR1-SG) containing coding regions of strictosidine synthase (STR1, EC 4.3.3.2) and strictosidine glucosidase (SG, EC 3.2.1.105) from the Indian medicinal plant Rauvolfia serpentina was constructed. Functional expression of the vector in Escherichia coli cells (M15 strain) was proven by isolation of prepurified enzyme extracts, which show both STR1 and SG activities. Incubation of the enzyme in the presence of tryptamine and secologanin delivered the indole alkaloid cathenamine, demonstrating functional co-expression of both STR1- and SG-cDNAs. Cathenamine reduction by sodium borohydride leading to tetrahydroalstonine revealed the chemo-enzymatic indole alkaloid synthe…

Symptoms, course, and complications of abdominal attacks in hereditary angioedema due to C1 inhibitor deficiency.

2006

Recurrent abdominal attacks belong to the cardinal and most distressing symptoms of hereditary angioedema (HAE) due to C1 inhibitor deficiency. They are characterized by crampy pain, but may include vomiting, diarrhea, and other features. Detailed clinical data about the symptoms and course of abdominal attacks have not been reported.We retrospectively observed a total of 33,671 abdominal attacks in 153 patients with HAE including a prospectively examined subgroup of 23 patients. Symptoms, course, frequency of attacks, and complications were analyzed.The relation of mild, moderate, and severe attacks was 1:1.4:5.6 in the prospective part of the study. Extra-abdominal symptoms preceded the a…

“Calcina e gesso e polvere di marmo”. Giacomo Serpotta e la plastica dello stucco tra Seicento e Settecento

2020

Il contributo prende in rassegna alcune delle principali opere prodotte dal maggiore stuccatore del Settecento siciliano, Giacomo Serpotta. Il percorso cronologico prende le mosse dalle esperienze della tradizione con gli apparati cinqucenteschi dei Ferraro e del padre Gaspare, proseguendo con le pietre miliari in cui la tecnica di Serpotta e la sua visione compositiva si evolvono. Sono affrontati anche alcuni aspetti tecnici costitutivi e costruttivi degli apparati.

3-Oxo-rhazinilam:  A New Indole Alkaloid from Rauvolfia serpentina × Rhazya stricta Hybrid Plant Cell Cultures

2000

A new monoterpenoid indole alkaloid, 3-oxo-rhazinilam (1), was isolated from intergeneric somatic hybrid cell cultures of Rauvolfia serpentina and Rhazya stricta, and the structure was determined by detailed 1D and 2D NMR analysis. It was also proved that 3-oxo-rhazinilam (1) is a natural constituent of the hybrid cells.

The neuronal efflux of noradrenaline: Dependency on sodium and facilitation by ouabain

1974

Rabbit hearts were isolated after pretreatment with the MAO inhibitor pargyline and with reserpine and were perfused with 200 ng/ml noradrenaline for 1 h. During the subsequent wash-out with an amine-free solution for 2 h, the neuronal efflux of noradrenaline declined mono-exponentially with a mean halftime of 42 min. Both Na+-free solution and ouabain caused facilitation of the efflux which thereafter declined in a multi-exponential fashion. The maximum facilitation was reached after 3 min of Na+-free perfusion and 25 min after introduction of ouabain. The amount of exogenous noradrenaline accumulated in the heart was only partially released when the extracellular Na+-concentration was nor…

ER+-derived breast cancer stem cells reveal a high expression of the serpin protease inhibitor PI-9.

2015

Introduction: Breast cancers (BC) are the major cause of death in women. More than 70% of BCs express high levels of estrogen receptor-α (ERα) and are sustained for their growth by the hormone. Estrogens seem to protect BC cells from apoptosis mediated by immunosurveillance associated with cytotoxic T lymphocytes and NK cells granzyme B release. However, the production of granzyme B inhibitor PI-9 by tumor cells causes a short-circuit in immunosurveillance’s signalling. Although it has been shown the role of PI-9 in BC cells, its presence has not been investigated in tumor stem cells so far. Methods: Cell viability was evaluated by MTT, cell cycle by propidium iodide staining; mRNA and prot…

Acute Myocardial Infarction and Proinflammatory Gene Variants

2007

We identified four genetic risk sets for acute myocardial infarction (AMI) from information on functional gene variants that favor inflammation or modulate cholesterol metabolism: IL6 -174 G/C, TNF -308 G/A, IL10 -1082 G/A, SERPINA3 -51 G/T, IFNG +874 T/A, HMGCR -911 C/A, and APOE ε2/3/4; 316 patients and 461 healthy subjects, all Italian. Putative risk alleles are shown underlined. The sets were identified using grade-of-membership analysis. Membership scores in the sets are automatically generated for individuals. The ''low intrinsic risk'' set had alleles that downregulate inflammation and cholesterol synthesis (IL6, TNF, ILl0, HMGCR). ''AMI across a broad age range'' carried multiple pr…

Ecology of the eulophid parasitoid community living on hosts of spontaneous flora linked to citrus grove (Hymenoptera: Chalcidoidea: Eulophidae).

2001

A TEST of the NATURE of the FE K LINE in the NEUTRON STAR LOW-MASS X-RAY BINARY SERPENS X-1

2015

Broad Fe K emission lines have been widely observed in the X-ray spectra of black hole systems, and in neutron star systems as well. The intrinsically narrow Fe K fluorescent line is generally believed to be part of the reflection spectrum originating in an illuminated accretion disk, and broadened by strong relativistic effects. However, the nature of the lines in neutron star LMXBs has been under debate. We therefore obtained the longest, high-resolution X-ray spectrum of a neutron star LMXB to date with a 300 ks Chandra HETGS observation of Serpens X-1. The observation was taken under the "continuous clocking" mode and thus free of photon pile-up effects. We carry out a systematic analys…

Oltre l'illusionismo pittorico: i teatrini prospettici nell'architettura siciliana tra Seicento e Settecento

2015

Durante i primi trentanni del XVI secolo l'uso in Sicilia di composizioni scultoree in prospettiva subì un significativo sviluppo grazie all'opera di Antonello Gagini (1478ca-1536) che conferì un vero e proprio effetto tridimensionale alle immagini scolpite nel marmo dando luogo ai cosiddetti "teatrini prospettici". L'inizio artistico di Giacomo Serpotta (1656-1732) diede il via ad una nuova, breve ma significativa fase orientata verso nuovi effetti prospettici. During the first thirty of the 16th century the use in Sicily of perspective compositions in sculptural reliefs underwent significanti development thanks to the work of Antonello Gagini (1478ca-1536) who passed previous models and l…