Search results for "serp"
showing 10 items of 205 documents
Fosildiagénesis del anélido Rotularia spirulaea (Lamarck, 1818) (Polychaeta, Serpulidae) en el Eoceno del dominio pirenaico occidental
2018
Alpha-1 antitrypsin deficiency: outstanding questions and future directions
2018
BACKGROUND: Alpha-1 antitrypsin deficiency (AATD) is a rare hereditary condition that leads to decreased circulating alpha-1 antitrypsin (AAT) levels, significantly increasing the risk of serious lung and/or liver disease in children and adults, in which some aspects remain unresolved. METHODS: In this review, we summarise and update current knowledge on alpha-1 antitrypsin deficiency in order to identify and discuss areas of controversy and formulate questions that need further research. RESULTS: 1) AATD is a highly underdiagnosed condition. Over 120,000 European individuals are estimated to have severe AATD and more than 90% of them are underdiagnosed. CONCLUSIONS: 2) Several clinical and…
Estudio conductual, electrofisiológico e inmunohistoquímico del modelo de fibromialgia inducido por reserpina en rata
2016
El síndrome de fibromialgia (FMS) es una patología de etiología desconocida caracterizada por dolor musculoesquelético generalizado acompañado de otros síntomas entre los que destacan las alteraciones del sueño y los trastornos depresivos, que afecta gravemente a la calidad de vida de los pacientes. Tiene una prevalencia relativamente importante en nuestra sociedad lo que determina que tenga un elevado impacto socio-económico. El desconocimiento de la causa que la genera y los recientes cambios en los criterios diagnósticos contribuyen a que, a pesar de los avances, actualmente el tratamiento del FMS no consiga alcanzar un grado de eficacia suficientemente satisfactorio. Es necesario el est…
Un groupe statuaire complexe à Autun : essai d’identification
2012
Au cours d’un diagnostic réalisé au nord-ouest de la ville d’Autun, à proximité du rempart d’origine augustéenne, un fragment de groupe statuaire a été trouvé au croisement de deux rues antiques. Reposant sur le sol de circulation, il semble avoir été abandonné au cours de l’Antiquité tardive ou du haut Moyen Age. Il se compose d’un personnage cuirassé (cuirasse musclée) vraisemblablement représenté de dos. Il s’agirait d’une gigantomachie, en ronde-bosse (sculpté sur trois cotés), la face principale a été délitée par la cassure de l’arkose taillée en délit (les bancs géologiques locaux sont impuissants à fournir la hauteur nécessaire à ce groupe). Sur ce drapé, on voit se lover dans une at…
Venómica y antivenómica de venenos botrópicos del suroccidente de Colombia y Ecuador
2021
Bothrops asper es un complejo de especies de importancia clínica en Latinoamérica causante de aproximadamente entre el 50 a 80% de los envenenamientos ofídicos de tipo botrópico. La abundancia de individuos, los hábitats crípticos que frecuenta este grupo y su amplia distribución desde el Sur de México hasta el norte de Perú, explican la alta incidencia de accidentes ocasionados por estas serpientes. La integración de datos moleculares, morfológicos y geográficos sugieren una estructura filogeográfica compleja de la especie en Centro y norte de Suramérica, con la existencia de diferentes linajes, algunos con procesos de divergencia reciente, especialmente en los valles interandinos al suroc…
Utilizing genetically engineered bacteria to produce plant-specific glucosides
2001
Plant-derived glucosides have attracted much attention due to their widespread applications. This class of products is difficult to isolate or to synthesize in pure form because of the resulting low yields. Thus, simple approaches for the generation of such glucosides would be highly beneficial. We purified and characterized a novel glucosyltransferase from plant cell suspension cultures of Rauvolfia serpentina, which showed rather low substrate specificity. We obtained its cDNA and expressed the active recombinant protein in bacteria (Escherichia coli) with excellent plant-specific glucosylation efficiencies. Compared with the plant system, the bacteria delivered the new enzyme, which was …
�ber den Wirkungsmechanismus von Reserpin
1959
High speed X-ray analysis of plant enzymes at room temperature.
2013
X-ray measurements at room temperature (295 K) deliver high quality data sets with unprecedented speed (2 min), as shown for crystallized raucaffricine-O-β-D-glucosidase (RG), its mutant RG-Glu186Gln and several ligand complexes of the enzyme which participates in alkaloid biosynthesis in the plant Rauvolfia. The data obtained are compared with data sets measured under typical cryo conditions (100K). Under both conditions, density maps are highly comparable and favor the described protocol for room temperature measurements, potentially paving the way for future crystallographic studies capturing biosynthetic pathway intermediates.
Natural products and enzymes from plant cell cultures
1995
Plants represent an unlimited source of natural products. Many of the recently detected phytochemicals exhibit remarkable bioactivities, ranging from anticancer activity, phosphodiesterase inhibition to cytotoxicity against HIV-infected cells. Cultivated plant cells produce at their unorganized, dedifferentiated stage secondary metabolites, but in very different amounts in so far as new compounds are concerned. In fact, more than 140 novel natural products are presently known from plant cell cultures, which also include new metabolites formed by biotransformation. The biotransformation capacity of suspended cells is described and recent high yielding transformations, like the formation of a…
The gene encoding polyneuridine aldehyde esterase of monoterpenoid indole alkaloid biosynthesis in plants is an ortholog of theα/β hydrolase super fa…
2000
The biosynthesis of the anti-arrhythmic alkaloid ajmaline is catalysed by more than 10 specific enzymes. In this multistep process polyneuridine aldehyde esterase (PNAE) catalyses a central reaction by transforming polyneuridine aldehyde into epi-vellosimine, which is the immediate precursor for the synthesis of the ajmalane skeleton. PNAE was purified from cell suspension cultures of Rauvolfia serpentina. The N-terminal sequence and endoproteinase LysC fragments of the purified protein were used for primer design and for the amplification of specific PCR products leading to the isolation of PNAE-encoding cDNA from a R. serpentina library. The PNAE cDNA was fused with a C-terminal His-tag, …