Search results for "sheep"
showing 10 items of 279 documents
Imaging of VSOP labeled stem cells in agarose phantoms with susceptibility weighted and T2* weighted MR Imaging at 3T: determination of the detection…
2013
Objectives This study aimed to evaluate the detectability of stem cells labeled with very small iron oxide particles (VSOP) at 3T with susceptibility weighted (SWI) and T2* weighted imaging as a methodological basis for subsequent examinations in a large animal stroke model (sheep). Materials and Methods We examined ovine mesenchymal stem cells labeled with VSOP in agarose layer phantoms. The experiments were performed in 2 different groups, with quantities of 0–100,000 labeled cells per layer. 15 different SWI- and T2*-weighted sequences and 3 RF coils were used. All measurements were carried out on a clinical 3T MRI. Images of Group A were analyzed by four radiologists blinded for the num…
A pyrroloquinazoline derivative with anti-inflammatory and analgesic activity by dual inhibition of cyclo-oxygenase-2 and 5-lipoxygenase
2002
Abstract In a previous study, we reported a new pyrroloquinazoline derivative, 3-(4′-acetoxy-3′,5′-dimethoxy)benzylidene-1,2-dihydropyrrolo[2,1- b ]quinazoline-9-one (PQ), which inhibited human purified 5-lipoxygenase activity and prostaglandin E 2 release in lipopolysaccharide-stimulated RAW 264.7 cells. In the present work, we show that PQ inhibits cyclo-oxygenase-2 activity in intact cell assays (human monocytes) and purified enzyme preparations (ovine isoenzymes) without affecting cyclo-oxygenase-1 activity. This behaviour was confirmed in vivo by using the zymosan-injected mouse air pouch model, where PQ caused a marked reduction in cell migration and leukotriene B 4 levels at 4 h, as …
Estudio experimental comparado de marcadores bioquímicos en la infección por Fasciola hepatica y F. gigantica
2017
Fascioliasis is caused by the genetically and phenotypically very close Fasciola hepatica and F. gigantica. The latter, always considered secondary in human infection, appears nowadays increasingly involved in human endemic areas of Africa and Asia. Unfortunately, little is known about the pathogenicity of this liver fluke species, mainly due to difficulties assessing the moment of a patient's infection in the anamnesis and in the differential diagnosis with F. hepatica. This is the first experimental study comparing F. hepatica and F. gigantica in a long-term study of up to 24 weeks with genotypically and phenotypically standardised fluke strains in the same animal model host, the Guirra s…
Genome-wide analysis of Italian sheep diversity reveals a strong geographic pattern and cryptic relationships between breeds
2014
Summary Italy counts several sheep breeds, arisen over centuries as a consequence of ancient and recent genetic and demographic events. To finely reconstruct genetic structure and relationships between Italian sheep, 496 subjects from 19 breeds were typed at 50K single nucleotide polymorphism loci. A subset of foreign breeds from the Sheep HapMap dataset was also included in the analyses. Genetic distances (as visualized either in a network or in a multidimensional scaling analysis of identical by state distances) closely reflected geographic proximity between breeds, with a clear north–south gradient, likely because of high levels of past gene flow and admixture all along the peninsula. Sa…
Niegodziwi pasterze i Dobry Pasterz Ez 34 w świetle retoryki hebrajskiej
2020
Kontekstem podjętych badań było odmienne opisywanie struktury tekstu Ez 34 przez różnych komentatorów Księgi Ezechiela. Celem badań było odkrycie struktury, którą starożytny autor zaplanował i zapisał. Zastosowano metodę retoryki hebrajskiej, którą opracował Roland Meynet. Osiągnięto następujące wyniki badań: fragment Ez 34,1-10 ma strukturę paralelno-koncentryczną o schemacie: A, B, C, B’, A’. Fragment Ez 34,11-31 też ma strukturę paralelno-koncentryczną o takim samym schemacie. Wniosek, jaki można wyciągnąć z badanego tekstu, sprowadza się do stwierdzenia, że Księga Ezechiela jest aktualna dzisiaj.
Effect of substitution of barley and tickbean to maize and extruded soybean in the diet on milk and cheese from ewes grazing under two different stoc…
2007
This experiment aimed to verify the effects of a concentrate supplement based on barley and tickbean (BT), compared with a concentrate of maize and extruded soybean meal (MS), on milk and cheese yield and composition of ewes grazing with different stocking rate (SR). Thirty two Comisana ewes, divided into four groups, for 50 days in spring were allowed to graze plots of a mixed sward of Italian reygrass and berseem clover with a low (LSR, 23 ewes/ha) or a high SR (HSR, 38 ewes/ha) and fed BT or MS. Herbage allowance per ewe was more than double at LSR compared to HSR (P<0.001), and daily milk yield was 10 to 15% higher (P<0.01) in the LSR-BT ewes than in the other groups. Milk composition, …
Estudios estratégicos para la lucha contra la fascioliasis humana a nivel mundial = Strategic studies for the fight against de human fascioliasis wor…
2013
Fascioliasis is a zoonotic parasitic disease caused by Fasciola hepatica and F. gigantica. Emergence of human fascioliasis prompted a worldwide control initiative including a pilot study in several countries. Given the necessity to characterize the fasciolid populations involved, the overall aim of the present research is, first, the phenotypic characterization of faciolid adults and eggs implicated in human endemic areas, where this initiative has been implemented, through a computer image analysis system (CIAS) applied on the basis of standardized measurements. Second, new immunological techniques and their potential use as immunological diagnostic tests in those areas are evaluated. The …
THE ATTITUDE AND MOTIVATION OF BUYERS OF TRADITIONAL/ LOCAL/ BIO PRODUCTS IN THE CONTEXT OF AGROTOURISM, IN SIBIU COUNTY, ROMANIA
2013
Based on the statistical data concerning the sheep breeding in district Sibiu and on the information obtained by making investigations markets and fairs where they sell traditional/bio products, the extent was appreciated to which this products are known and desired. The survey was conducted between May and November 2012 in four locations: “Transilvania Peasant Market (Piata Taraneasca Transilvania)”, “Traditional agro-food products Fear (Targul de Produse Agroalimentare Traditionale)” from Huet Market, “Traditional Products and Handicrafts Fear (Targul de Produse Traditionale si Mestesugaresti)” inside the Carrefour hypermarket and in “Agricultural Market” Rasinari, Sibiu county. There wer…
Genomic diversity and population structure analysis reveal few genetic differences among Ethiopian indigenous sheep populations
2017
African sheep, like other domestic sheep, are domesticated from the Asiatic mouflon (Ovis orientalis). They entered the continent through the North and the Horn of Africa regions following maritime and terrestrial trading routes. Ethiopia is one of the main entry points of various plant and animal domesticates into Africa. It is characterised by diverse agro-ecologies, ancient human ethnic diversity and the presence of indigenous sheep breeds/populations of unprecedented morphological diversity (e.g. tail types). Here, we investigate the genome diversity and population structure of 146 unrelated animals from 11 Ethiopian indigenous sheep populations. DNA was extracted from ear tissue punche…
MutSa expression predicts a lower disease-free survival in malignant salivary gland tumors:an immunohistochemical study
2021
Appropriate DNA replication is vital to maintain cell integrity at the genomic level. Malfunction on DNA repair mechanisms can have implications related to tumor behavior. Our aim was to evaluate the expression of key complexes of the DNA mismatch-repair system MutS? (hMSH2-hMSH6) and MutS? (hMSH2-hMSH3) in a panel comprising the most common benign and malignant salivary gland tumors (SGT), and to determine their association with disease-free survival. Ten cases of normal salivary gland (NSG) and 92 of SGT (54 benign and 38 malignant) were retrieved. Immunohistochemistry was performed for hMSH2, hMSH3, hMSH6. Scanned slides were digitally analyzed based on the percentage of positive cells w…