Search results for "silencing"

showing 3 items of 253 documents

Study of the interactions between Broad bean wilt virus 1 and its host plants

Broad bean wilt virus 1 (BBWV-1) is the type member of the Fabavirus genus, in the Secoviridae family. BBWV-1 is worldwide distributed and infects important horticultural and ornamental crops causing considerable economic losses. However, information about the biological and molecular characteristics of BBWV-1 isolates is scarce. BBWV-1 is composed of two molecules of positive single-stranded RNA (ssRNA+) that are separately encapsidated in virions of icosahedral morphology. Each ssRNA+ encodes polyproteins which are processed by proteolytic cleavage into functional peptides. RNA1 (~ 5.8 kb) encodes for one polyprotein that renders proteins involved in viral replication: a protease cofactor…

viral infectious clone.biological characterizationpathogenicity determinantBroad bean wilt virus 1; biological characterization; pathogenicity determinant; suppressor of post-transcriptional gene silencing; viral infectious clone.Broad bean wilt virus 1suppressor of post-transcriptional gene silencing
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Characterization of the autoantigen La (SS-B) as a dsRNA unwinding enzyme

1997

During the analysis of the La (SS-B) autoantigen for catalytic activities an ATP-dependent double-stranded RNA unwinding activity was detected. Both native and recombinant La proteins from different species displayed this activity, which could be inhibited by monospecific anti-La antibodies. La protein was able to melt dsRNA substrates with either two 3'-overhangs or a single 3'- and a 5'-overhang. Double-stranded RNAs with two 5'-overhangs were not unwound, indicating that at least one 3'-overhang is required for unwinding. Sequence elements of the La protein that might be involved in dsRNA unwinding, such as an evolutionarily conserved putative ATP-binding motif and an element that is hom…

virusesgenetic processesGene ExpressionRNA-binding proteinBiologyAutoantigensAntibodiesSubstrate SpecificitySingle-stranded binding proteinlaw.inventionMiceAdenosine TriphosphatelawGene expressionEscherichia coliGeneticsAnimalsHumansGeneralLiterature_REFERENCE(e.g.dictionariesencyclopediasglossaries)Cells CulturedRNA Double-StrandedRibonucleoproteinRNARNA NucleotidyltransferasesProtein kinase RMolecular biologyRecombinant ProteinsRatsenzymes and coenzymes (carbohydrates)RNA silencingLiverRibonucleoproteinsbiology.proteinRecombinant DNAElectrophoresis Polyacrylamide GelRNA HelicasesResearch Article
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La organización del trabajo por proyectos: La fábrica de la precariedad laboral

2019

The project-based organization, considered as a radical reorganization of work around one or more simultaneous projects, is nowadays widespread in large companies and public administrations. According to the managerial way of thinking, this system would be virtuous: by giving more autonomy to the employees in the act of production and by developing their skills through mobility and interdisciplinarity of work teams, it would be able to arouse their full commitment. This study confirms in some way how this opinion has been imposed, but at the expense of precarious working conditions, increasing the dedication of employees and silencing any form of criticism. The research is based on a review…

work organisationprecariousness 119 1241137-7038 8537 Arxius de sociologia 525290 2019 40 7038146 La organización del trabajo por proyectos: La fábrica de la precariedad laboral Goussardbut at the expense of precarious working conditionsby giving more autonomy to the employees in the act of production and by developing their skills through mobility and interdisciplinarity of work teams [this system would be virtuous]Lucie The project-based organizationorganización del trabajoincreasing the dedication of employees and silencing any form of criticism. The research is based on a review of the literature and 50 interviews with technicianshealthconsidered as a radical reorganization of work around one or more simultaneous projects:SOCIOLOGÍA [UNESCO]is nowadays widespread in large companies and public administrations. According to the managerial way of thinkingProject-based organizationthis system would be virtuous: by giving more autonomy to the employees in the act of production and by developing their skills through mobility and interdisciplinarity of work teamssaludUNESCO::SOCIOLOGÍAit would be able to arouse their full commitment. This study confirms in some way how this opinion has been imposedLa fábrica de la precariedad laboral Goussard [1137-7038 8537 Arxius de sociologia 525290 2019 40 7038146 La organización del trabajo por proyectos]precariedadwhere project-based organization is widespread. The links between this organization of work and precariousness are one of the results that stand out after the investigation. trabajo por proyectosengineers and industrial design executives working in a large company in the French automotive industry
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