Search results for "skeleton"
showing 10 items of 418 documents
Efectos de la exposición crónica al etanol sobre el tráfico intracelular y citoesqueleto como factores implicados en la migración neuronal
2013
El consumo de etanol durante la gestación puede inducir una serie de alteraciones graves en el desarrollo del feto, la manifestación más extrema da lugar al Síndrome Alcohólico Fetal (SAF). La exposición prenatal al alcohol es la causa conocida y, además evitable, más importante de retraso mental en el mundo occidental. Además de déficits cognitivos, los niños con SAF presentan múltiples anomalías estructurales en el sistema nervioso central, como reducción de la masa cerebral, y a nivel celular, daños en la migración neuronal, en el proceso de formación de espinas dendríticas y establecimiento de sinapsis. En la actualidad, los mecanismos moleculares implicados en la teratogénesis inducida…
The angiopoietin-Tie2 pathway regulates Purkinje cell dendritic morphogenesis in a cell-autonomous manner.
2021
Neuro-vascular communication is essential to synchronize central nervous system development. Here, we identify angiopoietin/Tie2 as a neuro-vascular signaling axis involved in regulating dendritic morphogenesis of Purkinje cells (PCs). We show that in the developing cerebellum Tie2 expression is not restricted to blood vessels, but it is also present in PCs. Its ligands angiopoietin-1 (Ang1) and angiopoietin-2 (Ang2) are expressed in neural cells and endothelial cells (ECs), respectively. PC-specific deletion of Tie2 results in reduced dendritic arborization, which is recapitulated in neural-specific Ang1-knockout and Ang2 full-knockout mice. Mechanistically, RNA sequencing reveals that Tie…
Myelin Proteome Analysis: Methods and Implications for the Myelin Cytoskeleton
2012
Myelin, the multilayered membrane that enwraps and insulates neuronal axons for fast signal propagation, is a plasma membrane specialization of oligodendrocytes and Schwann cells in the central and peripheral nervous system, respectively. Here we provide our lab protocols for the puri fi cation of myelin from mouse brains and for gel-based and gel-free proteomic applications, as well as a brief discussion with respect to our current knowledge of the myelin cytoskeleton.
Aggregation versus Biological Activity in Gold(I) Complexes. An Unexplored Concept
2021
The aggregation process of a series of mono- and dinuclear gold(I) complexes containing a 4-ethynylaniline ligand and a phosphane at the second coordination position (PR3-Au-C≡CC6H4-NH2, complexes 1-5, and (diphos)(Au-C≡CC6H4-NH2)2, complexes 6-8), whose biological activity was previously studied by us, has been carefully analyzed through absorption, emission, and NMR spectroscopy, together with dynamic light scattering and small-angle X-ray scattering. These experiments allow us to retrieve information about how the compounds enter the cells. It was observed that all compounds present aggregation in fresh solutions, before biological treatment, and thus they must be entering the cells as a…
Proteins of Muscle and the Cytoskeleton
1994
The contractile elements of striated vertebrate skeletal muscle, the myofibrils, contain thin filaments, which are 6 nm in diameter and consist mainly of actin, and thicker myosin filaments with a diameter of 16 nm (Fig. 10.1). During muscle contraction, the filaments undergo a sliding movement relative to each other (sliding filament mechanism). This is brought about by the reversible formation of bridges between the myosin molecules and the actin filaments, which bind, change their conformation and then dissociate (bridge cycle). The required energy is supplied by the hydrolysis of ATP. The sliding distance (step size) per molecule of ATP hydrolysed is controversial; the most recent measu…
Eremophilane Derivatives with a Novel Carbon Skeleton from Ligularia veitchiana
1997
Abstract Ligulaverin A 1, an eremophilane derivative with a novel carbon skeleton, was isolated from the medicinal plant Ligularia veitchiana, along with four analogues (ligulaverin B 2, ligulaverin C 3, ligulaverin D 4 and ligulaverin E 5). Their structures were elucidated by NMR techniques and X-ray diffraction. The biosynthetic route to this nineteen-carbon skeleton is discussed. © 1997 Elsevier Science Ltd.
An Improved Skew Angle Detection and Correction Technique for Historical Scanned Documents Using Morphological Skeleton and Progressive Probabilistic…
2017
International audience; Skew detection is a crucial step for document analysis systems. Indeed, it represents one of the basic challenges, especially in case of historical documents analysis. In this paper, we propose a novel robust skew angle detection and correction technique. Morphological Skeleton is introduced to significantly reduce the amount of data to treat by removing the redundant pixels and keeping only the central curves of the image components. The proposed method then uses Progressive Probabilistic Hough Transform (PPHT) to identify image lines. A special procedure is finally applied in order to estimate the global skew angle of the document image from these detected lines. E…
Hand Detection and Tracking Using the Skeleton of the Blob for Medical Rehabilitation Applications
2012
This article presents an image processing application for hand detection and tracking using the 4-connected skeleton of the segmentation mask. The system has been designed to be used with techniques of virtual reality to develop an interactive application for phantom limb pain reduction in therapeutic treatments. One of the major contributions is the design of a fast and accurate skeleton extractor, that has proven to be faster than those available in the literature. The skeleton allows the system to precisely detect the position of all the interest points of the hand (namely the fingers and the hand center). The system, composed of both the hand detector and tracker, and the virtual realit…
Real-Time Human Pose Estimation from Body-Scanned Point Clouds
2015
International audience; This paper presents a novel approach to estimate the human pose from a body-scanned point cloud. To do so, a predefined skeleton model is first initialized according to both the skeleton base point and its torso limb obtained by Principal Component Analysis (PCA). Then, the body parts are iteratively clustered and the skeleton limb fitting is performed, based on Expectation Maximization (EM). The human pose is given by the location of each skeletal node in the fitted skeleton model. Experimental results show the ability of the method to estimate the human pose from multiple point cloud video sequences representing the external surface of a scanned human body; being r…
Gesture Modeling by Hanklet-Based Hidden Markov Model
2015
In this paper we propose a novel approach for gesture modeling. We aim at decomposing a gesture into sub-trajectories that are the output of a sequence of atomic linear time invariant (LTI) systems, and we use a Hidden Markov Model to model the transitions from the LTI system to another. For this purpose, we represent the human body motion in a temporal window as a set of body joint trajectories that we assume are the output of an LTI system. We describe the set of trajectories in a temporal window by the corresponding Hankel matrix (Hanklet), which embeds the observability matrix of the LTI system that produced it. We train a set of HMMs (one for each gesture class) with a discriminative a…