Search results for "smad"
showing 10 items of 81 documents
Role of TGF-b1 in type I collagen production in bronchial epithelial cells: effects on Smad7 inhibitory role?
2008
Airway epithelial cells play an important role in inflammatory, apoptotic and remodelling process associate with fibrosis and COPD. Transforming growth factor 1 (TGF-b1) is involved in airways remodelling by Smads signalling pathway. We investigated the role of TGF-b1 on type I collagen production and Smads (Smad 2-3-4-and 7) expression in bronchial epithelial cells (16HBE). Cells were treated with 1ng/ml and 10ng/ml of TGF-b1 for 0, 3 and 24 hours. With low dose of TGF-b1 we observed no significant variation on Smad2 mRNA expression for both times but a significant increased of Smad7 mRNA expression at 3h (p=0.0043) and a significant reduction of Smad3, Smad4 and Smad7 mRNA expression at 2…
The mitotic kinase Aurora-A promotes distant metastases by inducing epithelial-to-mesenchymal transition in ERα+ breast cancer cells
2013
In this study, we demonstrate that constitutive activation of Raf-1 oncogenic signaling induces stabilization and accumulation of Aurora-A mitotic kinase that ultimately drives the transition from an epithelial to a highly invasive mesenchymal phenotype in estrogen receptor α-positive (ERα(+)) breast cancer cells. The transition from an epithelial- to a mesenchymal-like phenotype was characterized by reduced expression of ERα, HER-2/Neu overexpression and loss of CD24 surface receptor (CD24(-/low)). Importantly, expression of key epithelial-to-mesenchymal transition (EMT) markers and upregulation of the stemness gene SOX2 was linked to acquisition of stem cell-like properties such as the ab…
CCL5(RANTES) inhibits TGFbeta1 induced collagen production in human bronchial epithelial cells by action of Smad protein
2009
Airway epithelial cells modulate bronchial remodelling in COPD and asthma. TGF-β1 up-regulation was observed in the bronchial epithelial cells of asthmatics and COPD patients. TGF-β is involved in airways remodelling mainly acting via the Smad pathway. Increased CCL5 was also observed in bronchi of patients with severe COPD. We investigated the effects of TGF-β1 on collagen type I, Smad 3-4 and 7 expression and the effects of CCL5 on TGF-β1-induced collagen production in human bronchial epithelial cells (16HBE).Cells were treated with 10ng/ml of TGF-β1, 10ng/ml of CCL5 and 10ng/ml of both TGF-β1 and CCL5 for 0,3 and 24 hours.TGF-b1 increased Smad3,Smad4 and collagen type I(p=0.0472, p=0.043…
The Activation Status of the TGF-β Transducer Smad2 Is Associated with a Reduced Survival in Gastrointestinal Cancers: A Systematic Review and Meta-A…
2019
Aberrant function of Smad2, a crucial member of transforming growth factor beta (TGF-β) signaling, is associated with the development of malignancies, particularly in the gastrointestinal district. However, little is known about its possible prognostic role in such tumor types. With the first meta-analysis on this topic, we demonstrated that the lack of the activated form of Smad2 (phosphor-Smad2 or pSmad2), which was meant to be the C-terminally phosphorylated form, showed a statistically significant association with an increased risk of all-cause mortality in patients with gastrointestinal cancers (RR, 1.58; 95% CI, 1.05–2.37, p = 0.029, I2 = 84%), also after having adjusted for potential…
Role of cyclooxygenase pathways in bowel fibrotic remodelling in a murine model of experimental colitis
2022
Abstract Objective Gut fibrosis occurs under chronic inflammation. This study examined the effects of different cyclooxygenase (COX) inhibitors on fibrosis in the inflamed colon. Methods Colitis was induced by 2,4-dinitrobenzenesulfonic acid (DNBS) in albino male Sprague–Dawley rats. After 6, 12 and 18 days, macroscopic and microscopic damage, collagen and elastic fibre content were examined. At day 6, pro-fibrotic factors (collagen I and III, hydroxyproline, fibronectin, matrix metalloproteinase-2 and -9), transforming growth factor-beta (TGF-β) signalling [TGF-β, Ras homolog gene family member A (RhoA), phosphorylated small mother against decapentaplegic (pSMAD)-2 and -6] and peristalsis …
TGFbeta signaling: roles of Smads
2009
Individual variation of form of the brain of Triton cristatus Laur. and its relation to the specific variation of the brain of Urodela
1931
Smad7 controls resistance of colitogenic T cells to regulatory T cell-mediated suppression.
2008
Background & Aims Foxp3-expressing regulatory T cells (Tregs) play a key role in the maintenance of the gut immune homeostasis, and an intact transforming growth factor (TGF)-β signaling is required for their function. In inflammatory bowel disease (IBD), the TGF-β signaling is impaired because of high expression of the inhibitory molecule Smad7. Although no intrinsic defects in Tregs function have been shown in IBD, it is still unknown whether colitogenic T cells are susceptible to Treg-mediated suppression. In this study, we have investigated whether IBD mucosal CD4+ T cells are resistant to Tregs and whether Smad7 is involved in this process. Methods IBD lamina propria mononuclear cells …
Stresa pārvarēšanas stratēģiju saistība ar apmierinātību ar dzīvi cilvēkiem pēc vidēji smaga galvas smadzeņu bojājuma un cilvēkiem ar artrītu
2021
Pētījuma mērķis bija izpētīt saistību starp pielietoto stresa pārvarēšanas stratēģiju un apmierinātību ar dzīvi cilvēkiem, kuri piedzīvojuši vidēji smagu smadzeņu bojājumu, un cilvēkiem ar artrītu. Pētījumā piedalījās 131 dalībnieki vecumā no 17 līdz 84 gadiem. Dalībnieki tika iedalīti trīs respondentu grupās – 50 personas ar diagnosticētu artrītu (46 sievietes un 4 vīrieši), 31 personas, kurām dzīves laikā diagnosticēts vidēji smags galvas smadzeņu bojājums (17 sievietes un 14 vīrieši), un 50 personas, kurām nav bijis nekāda veida galvas smadzeņu bojājums un nav hroniskas saslimšanas (36 sievietes un 14 vīrieši). Pētījuma dalībnieki tika atlasīti pēc pieejamības un brīvprātības principa. A…
Radioloģiskā atradne magnētiskās rezonanses izmeklējumos pacientiem ar galvassāpēm
2016
Ievads. Mokošas galvassāpes ir viena no biežāk sastopamajām sūdzībām, kuru dēļ pacienti vēršas pēc palīdzības. 75% iedzīvotāju sūdzas par hroniskām vai periodiskām galvassāpju epizodēm. Tomēr šis procentuālais pacientu skaits ir tikai aptuvens. Pētījuma laikā tiks noskaidrots, kāds ir šo galvassāpju iemesls VSIA “PSKUS” ambulatorajiem pacientiem. Darba mērķis. Prospektīvā pētījumā ar magnētiskās rezonanses palīdzību analizēt VSIA “PSKUS” ambulatoros pacientus ar nosūtīšanas diagnozi ‘cefaļģija’ vai aizdomas par migrēnu. Noteikt atrasto patoloģiju vai attīstības variantu (anomāliju). Materiāli un metodes. Tika veikts aprakstošs prospektīvs pētījums. Pētījumam tika atlasīti pacienti ar nosūtī…