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Linee guida per la diagnosi dei profili di dislessia e disortografia previsti dalla legge 170: invito a un dibattito

2014

LA COMPETENZA MATERNA NELLE CONDIZIONI DI NASCITA PRETERMINE: UN Q-SORT PER L'ASSESSMENT E IL SUPPORTO

2019

In riferimento alla condizione di rischio della competenza genitoriale nella nascita pretermine, si pone la necessità di prendere in carico proprio l’esigenza di supportare e accompagnare le madri nel fronteggiamento dei nuovi compiti evolutivi posti dalla nascita prematura del proprio bambino, e quindi, di sostenere la competenza genitoriale; in tal senso, le autrici e il gruppo di ricerca di riferimento hanno costruito e validato, nel corso degli anni, uno specifico Q-sort sulla competenza materna percepita

Fixed fuzzy points of fuzzy mappings in Hausdorff fuzzy metric spaces with application

2015

Recently, Phiangsungnoen et al. [J. Inequal. Appl. 2014:201 (2014)] studied fuzzy mappings in the framework of Hausdorff fuzzy metric spaces. Following this direction of research, we establish the existence of fixed fuzzy points of fuzzy mappings. An example is given to support the result proved herein; we also present a coincidence and common fuzzy point result. Finally, as an application of our results, we investigate the existence of solution for some recurrence relations associated to the analysis of quicksort algorithms.

Il consorzio del vino Marsala. Una lettura economico-aziendale della sua storia, evoluzione, declino e possibili scenari futuri.

2020

Since the 1940s, the first initiatives take places to create the Marsala wine consortium. In 1963, the constitution of the consortium actually occurs, making Marsala the only Italian wine whose rules of operation are derived from a state law. For almost 50 years the Consortium was a point of reference for producers, amd actively contributed to the qualitative development of the image of Marsala. However, in 2016, after a glorious hustory, the Marsala Consortium no longer appears in the official ministerial list of consortia of Italiam DOC wines. The main reasons for this, illustrated in the paper, can be traced to the drastic reduction of the adherents to the consortium, to the contrasts th…

Performance organizzativa e carichi di lavoro nello studio sui Consorzi Regionali di Bonifica

2022

The objective of our research was to verify, through an empirical survey, the measuring of the relationship between workload and performance within complex public organizations. Starting from the reference literature the research approach adopted was of a mixed type characterized by an exploratory design and the analysis of a case study. #e data collection was carried out through documentary sources and some structured interviews administered to the management and the various sector heads of reclamation consortium studied. Based on the interviews carried out, a relationship between the measurement of the workload and the performance within the institution examined is inferred, because there…

The membrane anchor of microsomal epoxide hydrolase from human, rat, and rabbit displays an unexpected membrane topology.

1997

The microsomal epoxide hydrolase (mEH) and cytochrome P450s catalyze the sequential formation of carcinogenic metabolites. According to one algorithm for predicting the membrane topology of proteins, the human, the rabbit, and the rat mEH should adopt a type II topology. The type II topology is also predicted by a recently established neuronal network which is trained to recognize signal peptides with very high accuracy. In contrast to these predictions we find, based on N-glycosylation analysis in a cell-free and in a cellular system, that the membrane anchor of human, rat, and rabbit mEH displays a type I topology. This result is correctly predicted by the positive inside rule in which ne…

Apical transport of osteopontin is independent of N-glycosylation and sialylation.

2002

Studies of how epithelial surface polarity into apical and basolateral domains is generated and maintained have proposed that carbohydrate modifications serve as apical targeting signals for proteins by interacting with lectin sorters. However, the experimental evidence in support of N-glycans, O-glycans and sialic acids mediating apical transport is still very controversial. This could be partly due to the fact that in most studies exogenously expressed proteins were analysed. One has, therefore, examined the role of carbohydrate moieties in apical targeting of the endogenous secretory protein osteopontin in MDCK cells. It was found, however, that sorting of osteopontin does not require N-…

Transporter (TAP)- and proteasome-independent presentation of a melanoma-associated tyrosinase epitope.

2000

The melanosomal protein tyrosinase is considered as a target of specific immunotherapy against melanoma. Two tyrosinase-derived peptides are presented in association with HLA-A2.1 [Wolfel et al., Eur. J. Immunol., 24, 759-764 (1994)]. Peptide 1-9 (MLLAVLYCL) is generated from the putative signal sequence. The internal peptide 369-377 is posttranslationally converted at residue 371, and its presentation is dependent on functional TAP transporters and proteasomes [Mosse et al., J. exp. Med.187, 37-48 (1998)]. Herein, we report on the processing and transport requirements for the signal sequence-derived peptide 1-9 that were studied in parallel to those for peptide 369-377. After infection of …

The microsomal epoxide hydrolase has a single membrane signal anchor sequence which is dispensable for the catalytic activity of this protein

1994

The microsomal epoxide hydrolase (mEH) catalyses the hydrolysis of reactive epoxides which are formed by the action of cytochromes P-450 from xenobiotics. In addition it has been suggested that mEH might mediate the transport of bile acids. For the mEH it has been shown that it is co-translationally inserted into the endoplasmic reticulum. Here we demonstrate that the N-terminal 20 amino acid residues of this protein serve as its single membrane anchor signal sequence and that the function of this sequence can also be supplied by a cytochrome P-450 (CYP2B1) anchor signal sequence. The evidence supporting this conclusion is as follows: (i) the rat mEH and a CYP2B1-mEH fusion protein, in whic…

Efficient production of active chicken avidin using a bacterial signal peptide in Escherichia coli

2004

Chicken avidin is a highly popular tool with countless applications in the life sciences. In the present study, an efficient method for producing avidin protein in the periplasmic space of Escherichia coli in the active form is described. Avidin was produced by replacing the native signal sequence of the protein with a bacterial OmpA secretion signal. The yield after a single 2-iminobiotin–agarose affinity purification step was approx. 10 mg/l of virtually pure avidin. Purified avidin had 3.7 free biotin-binding sites per tetramer and showed the same biotin-binding affinity and thermal stability as egg-white avidin. Avidin crystallized under various conditions, which will enable X-ray cryst…