Search results for "sort"

Specific binding of VegT mRNA localization signal to membranes in Xenopus oocytes

2021

Abstract We have studied the interaction of a VegT mRNA localization signal sequence with the membranes of the mitochondrial cloud in Xenopus oocytes, and the binding of the VegT mRNA signal sequence to the lipid raft regions of the vesicles bounded by ordered and disordered phospholipid bilayers. RNA preference for the membranes of the mitochondrial cloud was confirmed using microscopy of a fluorescence resonance energy transfer from RNA molecules to membranes. Our studies show that VegT mRNA has a higher affinity for ordered regions of lipid bilayers. This conclusion is supported by the dissociation constant measurements for RNA-liposome complex and the visualization of the FRET signal be…

A Generalisation of the Mean-Variance Analysis

2009

In this paper we consider a decision maker whose utility function has a kink at the reference point with different functions below and above this reference point. We also suppose that the decision maker generally distorts the objective probabilities. First we show that the expected utility function of this decision maker can be approximated by a function of mean and partial moments of distribution. This ‘mean-partial moments’ utility generalises not only mean-variance utility of Tobin and Markowitz, but also mean-semivariance utility of Markowitz. Then, in the spirit of Arrow and Pratt, we derive an expression for a risk premium when risk is small. Our analysis shows that a decision maker i…

Efficient selection of silenced primary cells by flow cytometry

2007

Background: RNA interference has emerged as a new and potent tool to knockdown the expression of target genes and to investigate their functions. For short time experiments with mammalian cell lines, RNA interference is typically induced by transfecting small interfering RNAs (siRNAs). Primary cells constitute important experimental systems in many studies because of their similarity to their in vivo counterparts; however, transfection of these cells has been found to be difficult. As a consequence, RNA interference of primary cells may result in mixed phenotypes because of the simultaneous presence in the same preparation of transfected and nontransfected cells. This may be particularly in…

Les débuts dans la vie active des jeunes issus de l'immigration après des études supérieures

2004

04039; Les auteurs décrivent ici les débuts dans la vie active des jeunes issus de l'immigration d'Afrique du Nord (Algérie, Maroc, Tunisie) et d'Europe du Sud (Espagne, Italie, Portugal) qui quittent la formation initiale après une expérience des études supérieures. A l'aide des données de l'enquête "Génération 98" du Céreq, l'entrée dans la vie professionnelle de ces deux catégories d'anciens étudiants est comparée au devenir professionnel des jeunes d'origine française. Ce travail débute avec une présentation de la classification des origines nationales telle qu'elle est appliquée dans l'étude. Il est suivi d'un exposé des caractéristiques sociodémographiques et scolaro-universitaires de…

On Fuzziness, Its Homeland and Its Neighbour

2013

It has been frequently remembered (also in the invitation letter to contribute to the present volume) that in his 1962 paper [27] which is a sort of proto-manifesto of fuzzy sets [28], Zadeh among the possible names that could denote the new notion he was trying to introduce, basides fuzzy, mentioned the term cloudy. If he had chosen this last one as the name of the theory, today we had contributed to a volume On Cloudiness.

Suomen yleiskartta

1863

Suffix array and Lyndon factorization of a text

2014

Abstract The main goal of this paper is to highlight the relationship between the suffix array of a text and its Lyndon factorization. It is proved in [15] that one can obtain the Lyndon factorization of a text from its suffix array. Conversely, here we show a new method for constructing the suffix array of a text that takes advantage of its Lyndon factorization. The surprising consequence of our results is that, in order to construct the suffix array, the local suffixes inside each Lyndon factor can be separately processed, allowing different implementative scenarios, such as online, external and internal memory, or parallel implementations. Based on our results, the algorithm that we prop…

A failure of high level verbal response selection in progressive dynamic aphasia.

2005

Different theoretical interpretations have been offered in order to account for a specific language impairment termed dynamic aphasia. We report a patient (CH) who presented with a dynamic aphasia in the context of nonfluent progressive aphasia. CH had the hallmark of reduced spontaneous speech in the context of preserved naming, reading, and single word repetition and comprehension. Articulatory and grammatical difficulties were also present. CH had a very severe verbal generation impairment despite being able to describe pictorial scenes and action sequences well. In the experimental investigations CH was severely impaired in word, phrase, and sentence generation tasks when many competing…

Pyrrolomycins as antimicrobial agents. Microwave-assisted organic synthesis and insights into their antimicrobial mechanism of action

2019

Abstract New compounds able to counteract staphylococcal biofilm formation are needed. In this study we investigate the mechanism of action of pyrrolomycins, whose potential as antimicrobial agents has been demonstrated. We performed a new efficient and easy method to use microwave organic synthesis suitable for obtaining pyrrolomycins in good yields and in suitable amount for their in vitro in-depth investigation. We evaluate the inhibitory activity towards Sortase A (SrtA), a transpeptidase responsible for covalent anchoring in Gram-positive peptidoglycan of many surface proteins involved in adhesion and in biofilm formation. All compounds show a good inhibitory activity toward SrtA, havi…

Discovery and structure-activity relationship studies of irreversible benzisothiazolinone-based inhibitors against Staphylococcus aureus sortase A tr…

2014

Gram-positive bacteria, in general, and staphylococci, in particular, are the widespread cause of nosocomial and community-acquired infections. The rapid evolvement of strains resistant to antibiotics currently in use is a serious challenge. Novel antimicrobial compounds have to be developed to fight these resistant bacteria, and sortase A, a bacterial cell wall enzyme, is a promising target for novel therapies. As a transpeptidase that covalently attaches various virulence factors to the cell surface, this enzyme plays a crucial role in the ability of bacteria to invade the host's tissues and to escape the immune response. In this study we have screened a small molecule library against rec…