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showing 10 items of 247 documents
Analysis of trematode parasite communities in fish eye lenses by pyrosequencing of naturally pooled DNA.
2011
Infections by multiple parasite species are common in nature and have important consequences for between species interactions and coevolutionary dynamics with the host populations. For example ecological and evolutionary factors underlying the structure of parasite communities determine the range of hosts a parasite can infect and set the basis for both evolution of host defences and parasite virulence as well as management of diseases. Studies investigating these factors have been facilitated in the recent past by genetic methods which surmount difficulties of traditional morphological taxonomy in identifying individual parasite species. Here we take a step further and present a novel meth…
First data on microflora of loggerhead sea turtle (Caretta caretta) nests from the coastlines of Sicily (Italy)
2020
ABSTRACT Caretta caretta is threatened by many dangers in the Mediterranean basin, but most are human-related. The purposes of this research were: (i) to investigate microflora in samples from six loggerhead sea turtle nests located on the Sicilian coast and (ii) to understand microbial diversity associated with nests, with particular attention to bacteria and fungi involved in failed hatchings. During the 2016 and 2018 summers, 456 eggs and seven dead hatchling from six nests were collected. We performed bacteriological and mycological analyses on 88 egg samples and seven dead hatchlings, allowing us to isolate: Fusarium spp. (80.6%), Aeromonas hydrophila (55.6%), Aspergillus spp. (27.2%) …
An annotated checklist of bryophytes of Europe, Macaronesia and Cyprus
2020
Introduction. Following on from work on the European bryophyte Red List, the taxonomically and nomenclaturally updated spreadsheets used for that project have been expanded into a new checklist for the bryophytes of Europe. Methods. A steering group of ten European bryologists was convened, and over the course of a year, the spreadsheets were compared with previous European checklists, and all changes noted. Recent literature was searched extensively. A taxonomic system was agreed, and the advice and expertise of many European bryologists sought. Key results. A new European checklist of bryophytes, comprising hornworts, liverworts and mosses, is presented. Fifteen new combinations are propo…
Molecular Phylogeny of Tintinnid Ciliates (Tintinnida, Ciliophora)
2012
We investigated the phylogeny of tintinnids (Ciliophora, Tintinnida) with 62 new SSU-rDNA sequences from single cells of 32 marine and freshwater species in 20 genera, including the first SSU-rDNA sequences for Amphorides, Climacocylis, Codonaria, Cyttarocylis, Parundella, Petalotricha, Undella and Xystonella, and 23 ITS sequences of 17 species in 15 genera. SSU-rDNA phylogenies suggested a basal position for Eutintinnus, distant to other Tintinnidae. We propose Eutintinnidae fam. nov. for this divergent genus, keeping the family Tintinnidae for Amphorellopsis, Amphorides and Steenstrupiella. Tintinnopsis species branched in at least two separate groups and, unexpectedly, Climacocylis branc…
New Insights into the Systematics of the Schoenoxiphium Clade (Carex, Cyperaceae)
2017
The Spanish Ministry of Science and Technology supported this research through the project CGL2012-38744 and CGL2016- 77401-P to M. Luceño (Principal Investigator), E. Maguilla, M. Escudero, S. Martín-Bravo, and T. Villaverde and an FPU fellowship (AP2012-2189) from the Spanish Government (Ministry of Education, Culture, and Sport) and a Synthesys grant (GB-TAF-2523) by the European Community Research Infrastructure Program to E.Maguilla.
Diversity and distribution of Phytophthora species in protected natural areas in Sicily
2019
: The aim of this study was to investigate the occurrence, diversity, and distribution of Phytophthora species in Protected Natural Areas (PNAs), including forest stands, rivers, and riparian ecosystems, in Sicily (Italy), and assessing correlations with natural vegetation and host plants. Fifteen forest stands and 14 rivers in 10 Sicilian PNAs were studied. Phytophthora isolations from soil and stream water were performed using leaf baitings. Isolates were identified using both morphological characters and sequence analysis of the internal transcribed spacer (ITS) region. A rich community of 20 Phytophthora species from eight phylogenetic clades, including three new Phytophthora taxa, was …
Regional-scale analysis of arbuscular mycorrhizal fungi: the case of Burgundy vineyards
2016
SPE IPM INRA UB; Aim : To improve knowledge of arbuscular fungal communities for a sustainable management in vineyards. Methods and results : In 16 plots across Burgundy under contrasted soil properties and agricultural practices, we assessed arbuscular mycorrhizal fungal (AMF) diversity in vine roots, using pyrosequencing of ribosomal Internal Transcribed Spacers (ITS). AMF sequences could be retrieved from all plots across Burgundy, both in organic and in conventional vineyards with high chemical inputs. Sequences from the survey were almost exclusively affiliated to molecular taxa in the Glomerales, including six “core species” found in all plots, corresponding to 77% of all sequences, s…
Phylogenetic placement of Cailliella praerupticola (Melastomataceae), a rare, monospecific lineage from Guinea, West Africa
2021
The genus Cailliella includes a single species, C. praerupticola, restricted to a sandstone plateau region in Coyah, Dubreka, Forecariah and Kindia Prefectures, Guinea, West Africa. Until its rediscovery in 2016, this species was known only by the type gathering made in 1937. We provide an emended description, photographs of living material, locality and habitat data, and a discussion of its conservation status. We further designate a lectotype for C. praerupticola. On the basis of morphology, Cailliella had been considered an isolated member of the tribe Melastomateae, possibly sister to Melastomastrum. We tested the classification of Cailliella by means of a phylogenetic analysis of C. pr…
Glomus ibericum, Septoglomus mediterraneum, and Funneliformis pilosus, three new species of arbuscular mycorrhizal fungi
2020
Three new arbuscular mycorrhizal fungal species—Glomus ibericum, Septoglomus mediterraneum, and Funneliformis pilosus—are described and illustrated. In the field, the three species were associated with roots of Ammophila arenaria (Poaceae), Elymus farctus (Poaceae), Otanthus maritimus (Asteraceae), and Echinophora spinosa (Apiaceae) colonizing maritime dunes located along the Mediterranean coast in eastern Spain. The novelty of these species is supported by morphological, molecular, and phylogenetic analyses. Single-species cultures of S. mediterraneum and F. pilosus were obtained using Trifolium repens as a host plant, both forming arbuscular mycorrhizae, whereas single-species cultures fr…
Symbiosis between river and dry lands: phycobiont dynamics on river gravel bars
2020
AbstractRiver gravel bars are dynamic and heterogeneous habitats standing on transition between aquatic and terrestrial environment. Periodical flooding, low nutrient content, frost, missing safe sites, drought, and heat on the ground surface significantly influence life in these habitats. Mutualistic symbiosis may be a successful strategy for organisms to survive and to proliferate under harsh conditions. The lichen genus Stereocaulon was selected as a model symbiotic system among the organisms living on river gravel bars. The aim of our work was to determine effect of this dynamic environment on a phycobiont (i.e., green eukaryotic photobiont) community structure. We analysed 147 Stereoca…