Search results for "spodoptera"
showing 10 items of 125 documents
Engineering of chicken avidin: a progressive series of reduced charge mutants.
1998
Avidin, a positively charged egg-white glycoprotein, is a widely used tool in biotechnological applications because of its ability to bind biotin strongly. The high pI of avidin (approximately 10.5), however, is a hindrance in certain applications due to non-specific (charge-related) binding. Here we report a construction of a series of avidin charge mutants with pIs ranging from 9.4 to 4.7. Rational design of the avidin mutants was based on known crystallographic data together with comparative sequence alignment of avidin, streptavidin and a set of avidin-related genes which occur in the chicken genome. All charge mutants retained the ability to bind biotin tightly according to optical bio…
Estudio de una superfamilia de genes de respuesta a patógenos (repat) en insectos
2012
El intestino de los insectos es una de las principales vías de entrada de entomopatógenos. Con el fin de minimizar los efectos negativos de los patógenos, el intestino de los insectos posee diferentes mecanismos: síntesis de péptidos antimicrobianos, producción de especies reactivas de oxígeno, activación de los sistemas de reparación y detoxificación, melanización, fagocitosis, apoptosis y la renovación celular, entre otros. Estudios de expresión tras la exposición a diferentes patógenos han mostrado cómo la expresión de ciertos genes varía en el intestino tras la infección. Previamente a la realización de esta tesis, se identificó, en el lepidóptero Spodoptera exigua, una familia de genes…
Increase in midgut microbiota load induces an apparent immune priming and increases tolerance to Bacillus thuringiensis
2010
Summary The insect immune system is comprised of both humoral and cellular components that are mobilized in response to parasitic or pathogenic infections. Activation of the immune response implies a consid- erable expenditure of energy and that is why insects rely on inducible pathways that are activated after coming into contact with the pathogenic agent. Known as immune priming, insects can prolong the activation of the immune response and transmit their immune status to the next generation. Starting from a laboratory colony of the lepidopteran Spodoptera exigua and using the lytic zone assay as a measure of the immune status, we selected for a sub-colony with high levels of immune activ…
Neuropeptidome regulation after baculovirus infection. A focus on proctolin and its relevance in locomotion and digestion
2020
AbstractBaculoviruses constitute a large group of invertebrate DNA viruses, predominantly infecting larvae of the insect order Lepidoptera. During a baculovirus infection, the virus spreads throughout the insect body producing a systemic infection in multiple larval tissues. Some behavioral and physiological changes in lepidopteran larvae have been described following a baculovirus infection and those changes could be connected with alterations in the host’s central nervous system (CNS). As a main component of the CNS, neuropeptides are small protein-like molecules functioning as neurohormones, neurotransmitters or neuromodulators. These peptides are involved in regulating animal physiology…
A proctolin-like peptide is regulated after baculovirus infection and mediates in caterpillar locomotion and digestion
2022
Baculoviruses constitute a large group of invertebrate DNA viruses, predominantly infecting larvae of the insect order Lepidoptera. During a baculovirus infection, the virus spreads throughout the insect body producing a systemic infection in multiple larval tissues, included the central nervous system (CNS). As a main component of the CNS, neuropeptides are small protein-like molecules functioning as neurohormones, neurotransmitters, or neuromodulators. These peptides are involved in regulating animal physiology and behavior and could be altered after baculovirus infection. In this study, we have investigated the effect of Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV) infection …
The Putative Metal Coordination Motif in the Endonuclease Domain of Human Parvovirus B19 NS1 Is Critical for NS1 Induced S Phase Arrest and DNA Damage
2011
The non-structural proteins (NS) of the parvovirus family are highly conserved multi-functional molecules that have been extensively characterized and shown to be integral to viral replication. Along with NTP-dependent helicase activity, these proteins carry within their sequences domains that allow them to bind DNA and act as nucleases in order to resolve the concatameric intermediates developed during viral replication. The parvovirus B19 NS1 protein contains sequence domains highly similar to those previously implicated in the above-described functions of NS proteins from adeno-associated virus (AAV), minute virus of mice (MVM) and other non-human parvoviruses. Previous studies have show…
Bracovirus derived genes in the genome of Spodoptera exigua Hubner (Lepidoptera: Noctuidae) and their role in host susceptibility to pathogens
2015
La asociación entre los himenópteros parasitoides, polydnavirus (PDV) y lepidópteros representa un modelo interesante para estudiar la transferencia horizontal de genes. Está bien documentado que miles de himenópteros parasitoides pertenecientes a las familias Braconidae e Ichneumonidae han domesticado virus simbióticos denominados respectivamente Bracovirus o Ichnovirus. El virus se inyecta junto con los huevos del parásito en el hemocele del lepidóptero huésped, donde se expresan proteínas específicas. Estas proteínas inhiben el sistema inmune del lepidóptero y detienen su desarrollo, lo que beneficia el desarrollo de los huevos y luego las larvas del parasitoide. En este sistema único, t…
Getting on host’s nerves: Baculovirus-associated changes in the neuronal system of Spodoptera exigua
2021
La família Baculoviridae constitueix un grup divers de virus entomopatogens amb un genoma d’ADN de doble cadena circular. Generalment, els baculovirus infecten en estadis larvaris dels ordres Lepidoptera, Hymenoptera i Diptera. Dins dels baculovirus, els nucleopoliedrovirus són els més comuns, tenint els seus virions oclosos dins d’una estructura proteica anomenada cos d’inclusió (OB) amb forma de poliedre. Els baculovirus tenen dues classes de virions, els virions oclosos dins dels OBs, que s’anomenen occlusion-derived viruses (ODVs); i els virions no oclosos que es coneixen com budded viruses (BVs). El cicle d’infecció pel baculovirus comença quan les larves ingerixen els OBs després d’al…
Cadherin fragments of Lepidopteran and Coleopteran species do not enhance toxicity of Cry1Ca and Vip3Aa proteins to Spodoptera exigua (Hübner) (Lepid…
2020
Bacillus thuringiensis Berliner 1915 (Bt) is an entomopathogenic bacterium used to control insect pest worldwide. During its life cycle, Bt produces different insecticidal proteins, among which Veg...
Antifeedant activity of neo-clerodane diterpenoids from Teucrium fruticans and derivatives of fruticolone
1999
The antifeedant activity of three neo-clerodane diterpenoids, fruticolone, isofruticolone and fruticolide from Teucrium fruticans was assessed using larvae of Spodoptera littoralis. Isofruticolone was one of the most potent of the Teucrium derived neo-clerodanes. Chemical modification of functional groups on fruticolone showed that its activity could be enhanced.