Search results for "spore"
showing 10 items of 252 documents
Combined Effects of 50 Hz Electromagnetic Field and SiO2 Nanoparticles on Oxidative Stress in Plant’s Gametic Cells / Zemfrekvences (50 Hz) Elekromag…
2015
Abstract The paper presents the results of combined effects of an extremely low frequency electromagnetic field (50 Hz) (ELF EMF) and SiO2 nanoparticles on fluorescence of plant gametic cells (immature microspores). The data were recorded by a BD FACSJazz® cell sorter after cell irradiation by blue laser (488 nm). A significant difference of fluorescence was observed between gametic cells after 1 hour incubation in suspension of SiO2 nanoparticles and the control gametic cells. It was observed that fluorescence intensity of gametic cell was higher with ELF EMF treatment in comparison to control cells, but it was statistically significant only for cells treated with electromagnetic radiation…
Microspore embryogenesis induced through in vitro anther culture of almond (Prunus dulcis Mill.)
2016
Anther culture is one of the most widely used methods to induce gametic embryogenesis. The aim of this investigation was to induce microspore embryogenesis in almond (Prunus dulcis Mill.), through this technique. Anthers were cultured at the vacuolated developmental stage, and seven cultivars, two culture media and two temperature treatments were assessed. Although evidence of the microspore induction was observed in all the genotypes and treatments tested (symmetrical nucleus division and multinucleated structures), calli were produced merely by anthers cultured in the medium P and the regeneration of embryos was detected only in anthers of the cultivars Filippo Ceo, Lauranne and Genco, pl…
Biological and microclimatic monitoring for conservation of cultural heritage: a case study at the De Rossi room of the Palatina library in Parma
2019
Biological particles in heritage-related indoor environments (museums, libraries, archives) represent a hazard to artifacts (biodeteriogenic action), operators and visitors’ health. The aim of the study was to evaluate environmental biological contamination and microclimate conditions in different periods of the year in De Rossi room of the historical Palatina Library in Parma. Microclimatic measurements were recorded continuously for a period ranging from 11 to 17 days in: January–February, May and September 2017. Monitoring of bacterial and fungal contamination was performed for air by active and passive sampling and by nitrocellulose membranes on shelves and manuscripts. Microorganisms w…
The life cicle of Plasmopara viticola
2000
Plasmopara viticola (Berk. & M. A. Curtis) Bed. & De Toni is an obligate biotrophic oomycetous organism which causes downy mildew of grape vine. An understanding of its life-cycle is essential in relation to the pathology and epidemiology, and for the development of better control of the disease. It overwinters as oospores within fallen leaves.
A new species of Pythium with inflated sporangia and coiled antheridia, isolated from India
2008
Pythium kashmirense sp. nov. was isolated from soil samples taken on the Himalayas at the height of 5300 feet in the Shivalik Hill Range of the northern Indian state of Jammu and Kashmir. The oomycete has filamentous-inflated type sporangia and its antheridial filaments form loose loops around the female gametangia, and coil around the oogonial stalks. The new species is closely related to Pythium plurisporium, Pythium periilum, Pythium inflatum, and Pythium folliculosum. All of these oomycetes have filamentous-inflated type sporangia. However, P. kashmirense has its own distinguishing characteristics which can easily differentiate it among these related species. The sequences of the intern…
Two ectomycorrhizal truffles, Tuber melanosporum and T. aestivum , endophytically colonise roots of non‐ectomycorrhizal plants in natural environments
2020
International audience; Serendipitous findings and studies on Tuber species suggest that some ectomycorrhizal fungi, beyond their complex interaction with ectomycorrhizal hosts, also colonise roots of nonectomycorrhizal plants in a loose way called endophytism. Here, we investigate endophytism of T. melanosporum and T. aestivum . We visualised endophytic T. melanosporum hyphae by fluorescent in situ hybridisation on nonectomycorrhizal plants. For the two Tuber species, microsatellite genotyping investigated the endophytic presence of the individuals whose mating produced nearby ascocarps. We quantified the expression of four T. aestivum genes in roots of endophyted, non‐ectomycorrhizal plan…
Isoëtes sabatina (IsoëtaceaeLycopodiophyta), a new aquatic species from central Italy
2013
The new species Isoetes sabatina is described and illustrated. Morphological, anatomical, and ecological characters are given. It differs from the similar I. echinospora by several characters, such as megaspore ornamentation and size, microspore size, velum extension and form, and also for its ecology. A table showing the main differential characters of the new species compared with those of the other European aquatic species is presented. So far, the species is known from a single locality (Bracciano Lake, central Italy), having a peculiar ecology and within the Mediterranean bioclimate.
A new species of Pythium with filamentous sporangia having pectinolytic activities, isolated in the Burgundy region of France.
2001
A new species, Pythium pectinolyticum (F-83.1), isolated from soil samples taken in the Burgundy region is being described here. This species is characterised by its filamentous non-inflated type of sporangia, smooth-walled mostly catenulate oogonia and very rare antheridia. This fungus is a very slow-growing organism on most of the solid media, but it grows well in liquid media and also on those containing pectin as the sole source of carbon. Morphological features are described here together with the sequences of the internal transcribed spacer 1 region of the nuclear ribosomal DNA of the fungus, its comparison with related species, and its pectinolytic behaviour.
STUDY ON ISOLATED MICROSPORE CULTURE IN CITRUS SINENSIS L. OSBECK CV. MORO, A BLOOD ORANGE CULTIVAR
2014
In vitro tissue culture represents a useful support for the advancement of Citrus breeding and propagation. Haploidy technology, that is the single-step development of complete homozygous genotypes from heterozygous parents through gametic embryogenesis, has already a huge impact on many relevant crops, representing an integral part in their breeding programmes (Germanà 2011a; 2011b). In order to increase the number of genotypes responding to gametic embryogenesis, in vitro isolated microspore culture of Citrus sinensis L. Osbeck cv. Moro, has been carried out, investigating the influence of the culture medium composition, and, particularly, of the plant growth regulator types and concentra…
Cloning and expression of genes involved in conidiation and surface properties of Penicillium camemberti grown in liquid and solid cultures.
2008
International audience; Based on bioinformatic data on model fungi, the rodA and wetA genes encoding, respectively, a RodA hydrophobin protein and the WetA protein involved in conidiation mechanisms, were PCR-cloned and characterized for the first time in Penicillium camemberti. These results, completed by a sequence of the brlA gene (available in GenBank), which encodes a major transcriptional regulator also involved in the conidiation mechanism, were used to compare, by qRT-PCR, the expression of the three genes in liquid and solid cultures in a synthetic medium. While expression of the brlA and wetA genes increased dramatically in both culture conditions after 4 days of growth, expressio…