Search results for "structural"
showing 10 items of 5047 documents
Agarose/κ-carrageenan-based hydrogel film enriched with natural plant extracts for the treatment of cutaneous wounds.
2020
Abstract Hydrogels for complex and chronic wound dressings must be conformable, absorb and retain wound exudates and maintain hydration. They can incorporate and release bioactive molecules that can accelerate the healing process. Wound dressings have to be in contact with the wound and epidermis, even for long periods, without causing adverse effects. Hydrogel dressing formulations based on biopolymers derived from terrestrial or marine flora can be relatively inexpensive and well tolerated. In the present article hydrogel films composed by agarose (1.0 wt%), κ-carrageenan at three different concentrations (0.5, 1.0 and 1.5 wt%) and glycerol (3.0 wt%) were prepared without recourse to cros…
Ciberacoso escolar, ajuste psicosocial e ideación suicida en la adolescencia
2019
ABTSRACT Cyberbullying has become a growing social concern among the scientific community and in society in general. The consequences of cyberbullying for the victim are quite serious; many indicators of social maladjustment have been found, with suicide ideation being especially noteworthy. From this perspective, the main objective of this study was to analyze the relationships between cybervictimization and suicide ideation in adolescent victims of cyberbullying through the psychosocial maladjustment variables of loneliness, depressive symptomatology, perceived stress, and psychological distress. Participants in this study were 1,062 adolescents (547 boys, 515 girls), ranging in age from …
Presentación: La razón de un rodeo
1981
Un análisis estructuralista presentado por Vidal Beneyto
1981
Reseña sobre el libro "Posibilidades y límites del análisis estructural: una investigación concreta en torno a lenguaje y poesía", que coordina Vidal-Beneyto. Referencia completa: Vidal-Beneyto, José (Ed.) (1981). Posibilidades y límites del análisis estructural: una investigación concreta en torno a lenguaje y poesía. Madrid: Editora Nacional, Cultura y Sociedad.
Vidal Beneyto (Comp.) (1981). Posibilidades y límites del análisis estructural: una investigación concreta en torno a lenguaje y poesía. Madrid: Edit…
1981
Reseña sobre el libro "Posibilidades y límites del análisis estructural: una investigación concreta en torno a lenguaje y poesía", que coordina Vidal-Beneyto. Referencia completa: Vidal-Beneyto, José (Ed.) (1981). Posibilidades y límites del análisis estructural: una investigación concreta en torno a lenguaje y poesía. Madrid: Editora Nacional, Cultura y Sociedad.
Irreversible formation of intermediate BSA oligomers requires and induces conformational changes.
2004
Understanding the relation between protein conformational changes and aggregation, and the physical mechanisms leading to such processes, is of primary importance, due to its direct relation to a vast class of severe pathologies. Growing evidence also suggests that oligomeric intermediates, which may occur early in the aggregation pathway, can be themselves pathogenic. The possible cytotoxicity of oligomers of non-disease-associated proteins adds generality to such suggestion and to the interest of studies of oligomer formation. Here we study the early stages of aggregation of Bovine Serum Albumin (BSA), a non pathogenic protein which has proved to be a useful model system. Dynamic light sc…
Glucagon fibril polymorphism reflects differences in protofilament backbone structure
2010
Amyloid fibrils formed by the 29-residue peptide hormone glucagon at different concentrations have strikingly different morphologies when observed by transmission electron microscopy. Fibrils formed at low concentration (0.25 mg/mL) consist of two or more protofilaments with a regular twist, while fibrils at high concentration (8 mg/mL) consist of two straight protofilaments. Here, we explore the structural differences underlying glucagon polymorphism using proteolytic degradation, linear and circular dichroism, Fourier transform infrared spectroscopy (FTIR), and X-ray fiber diffraction. Morphological differences are perpetuated at all structural levels, indicating that the two fibril class…
VCD studies on cyclic peptides assembled from L-α-amino acids and a trans-2-aminocyclopentane- or trans-2-aminocyclohexane carboxylic acid.
2010
The increasing interest in peptidomimetics of biological relevance prompted us to synthesize a series of cyclic peptides comprising trans-2-aminocyclohexane carboxylic acid (Achc) or trans-2-aminocyclopentane carboxylic acid (Acpc). NMR experiments in combination with MD calculations were performed to investigate the three-dimensional structure of the cyclic peptides. These data were compared to the conformational information obtained by electronic circular dichroism (ECD) and vibrational circular dichroism (VCD) spectroscopy. Experimental VCD spectra were compared to theoretical VCD spectra computed quantum chemically at B3LYP/6-31G(d) density functional theory (DFT) level. The good agreem…
Roles of a conserved proline in the internal fusion peptide of Ebola glycoprotein
2004
AbstractThe structural determinants underlying the functionality of viral internal fusion peptides (IFPs) are not well understood. We have compared EBOwt (GAAIGLAWIPYFGPAAE), representing the IFP of the Ebola fusion protein GP, and EBOmut (GAAIGLAWIPYFGRAAE) derived from a non-functional mutant with conserved Pro537 substituted by Arg. P537R substitution did not abrogate peptide-membrane association, but interfered with the ability to induce bilayer destabilization. Structural determinations suggest that Pro537 is required to preserve a membrane-perturbing local conformation in apolar environments.
Tight DNA-protein complexes isolated from barley seedlings are rich in potential guanine quadruplex sequences
2020
Background The concept of chromatin domains attached to the nuclear matrix is being revisited, with nucleus described as a set of topologically associating domains. The significance of the tightly bound to DNA proteins (TBP), a protein group that remains attached to DNA after its deproteinization should be also revisited, as the existence of these interactions is in good agreement with the concept of the topologically associating domain. The work aimed to characterize the DNA component of TBP isolated from barley seedlings. Methods The tight DNA-protein complexes from the first leaves, coleoptiles, and roots of barley seedlings were isolated by purification with chromatography on nitrocell…