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showing 10 items of 1268 documents
Increasing temperature alters the within-host competition of viral strains and influences virus genetic variability
2021
Environmental conditions can affect viral accumulation, virulence and adaptation, which have implications in the disease outcomes and efficiency of control measures. Concurrently, mixed viral infections are relevant in plants, being their epidemiology shaped by within-host virus–virus interactions. However, the extent in which the combined effect of variations in abiotic components of the plant ecological niche and the prevalence of mixed infections affect the evolutionary dynamics of viral populations is not well understood. Here, we explore the interplay between ecological and evolutionary factors during viral infections and show that isolates of two strains of Pepino mosaic potexvirus co…
Adaptation of turnip mosaic potyvirus to a specific niche reduces its genetic and environmental robustness
2020
Robustness is the preservation of the phenotype in the face of genetic and environmental perturbations. It has been argued that robustness must be an essential fitness component of RNA viruses owed to their small and compacted genomes, high mutation rates and living in ever-changing environmental conditions. Given that genetic robustness might hamper possible beneficial mutations, it has been suggested that genetic robustness can only evolve as a side-effect of the evolution of robustness mechanisms specific to cope with environmental perturbations, a theory known as plastogenetic congruence. However, empirical evidences from different viral systems are contradictory. To test how adaptation…
Systematic analysis of specific and nonspecific auxin effects on endocytosis and trafficking.
2021
Abstract The phytohormone auxin and its directional transport through tissues are intensively studied. However, a mechanistic understanding of auxin-mediated feedback on endocytosis and polar distribution of PIN auxin transporters remains limited due to contradictory observations and interpretations. Here, we used state-of-the-art methods to reexamine the auxin effects on PIN endocytic trafficking. We used high auxin concentrations or longer treatments versus lower concentrations and shorter treatments of natural indole-3-acetic acid (IAA) and synthetic naphthalene acetic acid (NAA) auxins to distinguish between specific and nonspecific effects. Longer treatments of both auxins interfere wi…
Prefoldins contribute to maintaining the levels of the spliceosome LSM2–8 complex through Hsp90 in Arabidopsis
2020
14 p.-7 fig.-2 tab.
Drosophila Evolution over Space and Time (DEST) : A New Population Genomics Resource
2021
Drosophila melanogaster is a leading model in population genetics and genomics, and a growing number of whole-genome datasets from natural populations of this species have been published over the last years. A major challenge is the integration of disparate datasets, often generated using different sequencing technologies and bioinformatic pipelines, which hampers our ability to address questions about the evolution of this species. Here we address these issues by developing a bioinformatics pipeline that maps pooled sequencing (Pool-Seq) reads from D. melanogaster to a hologenome consisting of fly and symbiont genomes and estimates allele frequencies using either a heuristic (PoolSNP) or a…
A haplotype-resolved, de novo genome assembly for the wood tiger moth (Arctia plantaginis) through trio binning
2020
ABSTRACT Background Diploid genome assembly is typically impeded by heterozygosity because it introduces errors when haplotypes are collapsed into a consensus sequence. Trio binning offers an innovative solution that exploits heterozygosity for assembly. Short, parental reads are used to assign parental origin to long reads from their F1 offspring before assembly, enabling complete haplotype resolution. Trio binning could therefore provide an effective strategy for assembling highly heterozygous genomes, which are traditionally problematic, such as insect genomes. This includes the wood tiger moth (Arctia plantaginis), which is an evolutionary study system for warning colour polymorphism. F…
How to reach optimal estimates of confidence intervals in microscopic counting of phytoplankton?
2021
Abstract Present practices in the microscopic counting of phytoplankton to estimate the reliability of results rely on the assumption of a random distribution of taxa in sample preparations. In contrast to that and in agreement with the literature, we show that aggregated distribution is common and can lead to over-optimistic confidence intervals, if estimated according to the shortcut procedure of Lund et al. based on the number of counted cells. We found a good linear correlation between the distribution independent confidence intervals for medians and those for parametric statistics so that 95% confidence intervals can be approximated by using a correction factor of 1.4. Instead, the rec…
Out in the open : behavior’s effect on predation risk and thermoregulation by aposematic caterpillars
2020
Abstract Warning coloration should be under strong stabilizing selection but often displays considerable intraspecific variation. Opposing selection on color by predators and temperature is one potential explanation for this seeming paradox. Despite the importance of behavior for both predator avoidance and thermoregulation, its role in mediating selection by predators and temperature on warning coloration has received little attention. Wood tiger moth caterpillars, Arctia plantaginis, have aposematic coloration, an orange patch on the black body. The size of the orange patch varies considerably: individuals with larger patches are safer from predators, but having a small patch is beneficia…
Flow Control of Fluid in Pipelines Using PID Controller
2019
In this paper, a PID controller is utilized in order to control the flow rate of the heavy oil in pipelines by controlling the vibration in a motor pump. A torsional actuator is placed on the motor pump in order to control the vibration on a motor and consequently controlling the flow rates in pipelines. The necessary conditions for the asymptotic stability of the proposed controller are validated by implementing the Lyapunov stability theorem. The theoretical concepts are validated utilizing numerical simulations and analysis, which proves the effectiveness of the PID controller in the control of flow rates in pipelines.
Modeling and control of uncertain nonlinear systems
2018
A survey of the methodologies associated with the modeling and control of uncertain nonlinear systems has been given due importance in this paper. The basic criteria that highlights the work is relied on the various patterns of techniques incorporated for the solutions of fuzzy equations that corresponds to fuzzy controllability subject. The solutions which are generated by these equations are considered to be the controllers. Currently, numerical techniques have come out as superior techniques in order to solve these types of problems. The implementation of neural networks technique is contributed in the complex way of dealing the appropriate coefficients and solutions of the fuzzy systems.