Search results for "teeri"
showing 10 items of 308 documents
Dispersion from Cα or NH: 4D experiments for backbone resonance assignment of intrinsically disordered proteins
2020
AbstractResonance assignment of intrinsically disordered proteins is remarkably challenging due to scant chemical shift dispersion arising from conformational heterogeneity. The challenge is even greater if repeating segments are present in the amino acid sequence. To forward unambiguous resonance assignment of intrinsically disordered proteins, we present iHACANCO, HACACON and (HACA)CONCAHA, three Hα-detected 4D experiments with Cα as an additional dimension. In addition, we present (HACA)CON(CA)NH and (HACA)N(CA)CONH, new 4D Hα-start, HN-detect experiments which have two NH dimensions to enhance peak dispersion in a sequential walk through C′, NH and HN, and provide more accurate NH/HN ch…
Predicting habitat suitability for Ixodes ricinus and Ixodes persulcatus ticks in Finland
2022
AbstractBackgroundTicks are responsible for transmitting several notable pathogens worldwide. Finland lies in a zone where two human-biting tick species co-occur:IxodesricinusandIxodespersulcatus. Tick densities have increased in boreal regions worldwide during past decades, and tick-borne pathogens have been identified as one of the major threats to public health in the face of climate change.MethodsWe used species distribution modelling techniques to predict the distributions ofI.ricinusandI.persulcatus,using aggregated historical data from 2014 to 2020 and new tick occurrence data from 2021. By aiming to fill the gaps in tick occurrence data, we created a new sampling strategy across Fin…
Host’s genetic background determines the outcome of reciprocal faecal transplantation on life-history traits and microbiome composition
2022
Abstract Background Microbes play a role in their host's fundamental ecological, chemical, and physiological processes. Host life-history traits from defence to growth are therefore determined not only by the abiotic environment and genotype but also by microbiota composition. However, the relative importance and interactive effects of these factors may vary between organisms. Such connections remain particularly elusive in Lepidoptera, which have been argued to lack a permanent microbiome and have microbiota primarily determined by their diet and environment. We tested the microbiome specificity and its influence on life-history traits of two colour genotypes of the wood tiger moth (Arctia…
Direct pathway cloning and expression of the radiosumin biosynthetic gene cluster
2023
Radiosumins are a structurally diverse family of low molecular weight natural products that are produced by cyanobacteria and exhibit potent serine protease inhibition. Members of this family are dipeptides characterized by the presence of two similar non-proteinogenic amino acids. Here we used a comparative bioinformatic analysis to identify radiosumin biosynthetic gene clusters from the genomes of 13 filamentous cyanobacteria. We used direct pathway cloning to capture and express the entire 16.8 kb radiosumin biosynthetic gene cluster from Dolichospermum planctonicum UHCC 0167 in Escherichia coli. Bioinformatic analysis demonstrates that radiosumins represent a new group of chorismate-der…
Mucin induces CRISPR-Cas defense in an opportunistic pathogen
2022
It is unknown what circumstances promote particular bacterial defenses against bacterial viruses (phages). Almeida & Hoikkala et al. show that mucin, derived from mucus, greatly accelerates CRISPR-Cas defenses against phage in an opportunistic pathogen. Parasitism by bacteriophages has led to the evolution of a variety of defense mechanisms in their host bacteria. However, it is unclear what factors lead to specific defenses being deployed upon phage infection. To explore this question, we co-evolved the bacterial fish pathogen Flavobacterium columnare and its virulent phage V156 in presence and absence of a eukaryotic host signal (mucin) for sixteen weeks. The presence of mucin leads to a …
Effects of whole-body vertical shock-type vibration on human ability for fine manual control
1991
The effects of vertical (z-axis) whole-body shock-type vibration on the ability for fine manual control were examined. The amplitudes and frequency of the shocks was varied, but a constant frequency-weighted acceleration of 1.25 m/s2 r.m.s. was maintained. The examination of the shock's effects was carried out using an experimental system that simulated the actual workplace of earth-moving machinery. Control was measured using a first-order pursuit tracking-test, in which a seated subject was asked to use both hands to direct a cursor on a monitor using a steering wheel. Although the magnitude of shocks (peak amplitude of 6-10 m/s2) and the number of shocks per unit time (shock cycle of 10-…
Perceptual-Cognitive Skills and Performance in Orienteering
2008
The goal was analysis of the perceptual-cognitive skills associated with sport performance in orienteering in a sample of 22 elite and 17 nonelite runners. Variables considered were memory, basic orienteering techniques, map reading, symbol knowledge, map-terrain-map identification, and spatial organisation. A computerised questionnaire was developed to measure the variables. The reliability of the test (agreement between experts) was 90%. Findings suggested that competence in performing basic orienteering techniques efficiently was a key variable differentiating between the elite and the nonelite athletes. The results are discussed in comparison with previous studies.
Warm and Supportive Parenting Can Discourage Offspring’s Civic Engagement in the Transition to Adulthood
2016
It is widely believed that warm and supportive parenting fosters all kinds of prosocial behaviors in the offspring, including civic engagement. However, accumulating international evidence suggests that the effects of family support on civic engagement may sometimes be negative. To address this apparent controversy, we identified several scenarios for the negative effects of supportive parenting on youth civic engagement and tested them using four waves of data from the Finnish Educational Transitions Studies. They followed 1549 students (55 % female) from late adolescence into young adulthood, included both maternal (n = 231) and offspring reports of parental support, and assessed civic en…
Improving Quality of Care in Inflammatory Bowel Disease Through Patients’ Eyes: IQCARO Project
2019
Abstract Background Quality improvement is a major topic in inflammatory bowel disease (IBD) care, and measuring quality of care (QoC) is necessary for QoC improvement. Most QoC projects or consensus statements are designed from the health care professional point of view. Having QoC indicators designed for and fully evaluable by patients may provide a key tool for external evaluation of QoC improvement measures. The aim of the IQCARO project was to identify indicators to measure QoC from the IBD patient’s point of view. Methods An extensive review of the literature to identify indicators of QoC was performed; first the identified indicators were reviewed by a steering committee including pa…
An Agent-Based Model of Extortion Racketeering
2016
Mafias can be considered as criminal organisations that are in the business of producing, promoting, and selling protection. Here, we describe the Palermo Scenario, an agent-based model of protection rackets aimed to deepen our understanding of protection rackets, and help policymakers to evaluate methods for destabilising them. Additionally, since the system is explicitly specified, we can use it to investigate the entire causal pathway from cause to effect: not only from actions to Mafia destabilisation, but also the intermediate actions along the path and actors' internal mental representations among the population.