Search results for "teeri"
showing 10 items of 308 documents
Alternative Biosynthetic Starter Units Enhance the Structural Diversity of Cyanobacterial Lipopeptides
2019
Puwainaphycins (PUWs) and minutissamides (MINs) are structurally analogous cyclic lipopeptides possessing cytotoxic activity. Both types of compound exhibit high structural variability, particularly in the fatty acid (FA) moiety. Although a biosynthetic gene cluster responsible for synthesis of several PUW variants has been proposed in a cyanobacterial strain, the genetic background for MINs remains unexplored. Herein, we report PUW/MIN biosynthetic gene clusters and structural variants from six cyanobacterial strains. Comparison of biosynthetic gene clusters indicates a common origin of the PUW/MIN hybrid nonribosomal peptide synthetase and polyketide synthase. Surprisingly, the biosynthet…
Characterization of the interaction of the antifungal and cytotoxic cyclic glycolipopeptide hassallidin with sterol-containing lipid membranes.
2019
Hassallidins are cyclic glycolipopeptides produced by cyanobacteria and other prokaryotes. The hassallidin structure consists of a peptide ring of eight amino acids where a fatty acid chain, additional amino acids, and sugar moieties are attached. Hassallidins show antifungal activity against several opportunistic human pathogenic fungi, but does not harbor antibacterial effects. However, they have not been studied on mammalian cells, and the mechanism of action is unknown. We purified hassallidin D from cultured cyanobacterium Anabaena sp. UHCC 0258 and characterized its effect on mammalian and fungal cells. Ultrastructural analysis showed that hassallidin D disrupts cell membranes, causin…
Unraveling the pleomorphic forms of Borrelia burgdorferi
2014
Borrelia burgdorferi bakteeri on puutiaisten välittämän borrelioosin taudinaiheuttaja. Tämä tavanomaisesti spirokeettana esiintyvä bakteeri kykenee vaihtamaan muotoaan ympäristöolosuhteiden muuttuessa epäsuotuisiksi. Borrelia-bakteerin eri esiintymismuotojen uskotaan olevan borrelioosin pitkittyneen taudinkuvan takana, minkä vuoksi näiden muotojen tutkiminen on tärkeää. Tämän pro gradu –työn tavoitteena oli tutkia B. burgdorferin pleomorfisia muotoja ja löytää muotoja erottavia morfologisia tekijöitä sekä eroja proteiiniekspressiossa ja borrelioosipotilasseerumivasteessa spirokeettojen ja pyöreän esiintymismuodon välillä. Olettamuksena oli, että konfokaalimikroskopialla muotojen väliltä löy…
Suomalaisessa siipikarjassa ja tarhariistalinnuissa todettujen Brachyspira-suvun bakteereiden karakterisointia
2006
Thiourea Organocatalysts as Emerging Chiral Pollutants: En Route to Porphyrin-Based (Chir)Optical Sensing
2021
Environmental pollution with chiral organic compounds is an emerging problem requiring innovative sensing methods. Amino-functionalized thioureas, such as 2-(dimethylamino)cyclohexyl-(3,5-bis(trifluoromethyl)phenyl)thiourea (Takemoto’s catalyst), are widely used organocatalysts with virtually unknown environmental safety data. Ecotoxicity studies based on the Vibrio fischeri luminescence inhibition test reveal significant toxicity of Takemoto’s catalyst (EC50 = 7.9 mg/L) and its NH2-substituted analog (EC50 = 7.2–7.4 mg/L). The observed toxic effect was pronounced by the influence of the trifluoromethyl moiety. En route to the porphyrin-based chemosensing of Takemoto-type thioureas, their s…
”Meillä ei ole politiikkaa valtionavustuksissa!”
2021
Valtionavustustoiminta on olennainen osa suomalaista yhteiskuntapolitiikkaa ja valtionhallinnon ohjausjärjestelmää. Avustamista toimeenpanevat ministeriöt, tulosohjatut virastot sekä muut asiantuntijaorganisaatiot. Artikkelimme tutkii kulttuurihallinnon virkakunnan vallankäyttöä opetus- ja kulttuuriministeriön (OKM) valtionavustuspolitiikassa. Ymmärrämme kulttuurihallinnon ohjausvälineineen merkittävänä valtaa käyttävänä organisaatiorakenteena. Tarkastelu pohjautuu arviointitutkimukseen OKM:n tulosohjaaman asiantuntijaviraston Taiteen edistämiskeskuksen (Taike) valtionavustusprosessista. Artikkelimme on tapaustutkimus, jossa kuvaamme kulttuuripolitiikan toimeenpanorakent…
Social Participation Through Volunteering as Co-production of Public Services: Case Study of Latvia
2016
Active civic and social participation is considered to be significantly important for a country`s development in democratic societies. One of the popular forms of active citizenship is volunteering – the third economy sector that produces civic goods and/or services in which the public and private sectors are weak or inactive. In this aspect, volunteering is also a means for organized civil society to participate in the production of public services i.e. co-production. The aim of this research is the exploration of social participation in Latvia through volunteering, including its legal regulation and organizational setup, and identification of the aspects and prospects of volunteering in L…
Decreased temperature increases the expression of a disordered bacterial late embryogenesis abundant (LEA) protein that enhances natural transformati…
2021
Late embryogenesis abundant (LEA) proteins are important players in the management of responses to stressful conditions, such as drought, high salinity, and changes in temperature. Many LEA proteins do not have defined three-dimensional structures, so they are intrinsically disordered proteins (IDPs) and are often highly hydrophilic. Although LEA-like sequences have been identified in bacterial genomes, the functions of bacterial LEA proteins have been studied only recently. Sequence analysis of outer membrane interleukin receptor I (BilRI) from the oral pathogen Aggregatibacter actinomycetemcomitans indicated that it shared sequence similarity with group 3/3b/4 LEA proteins. Comprehensive …
Social Space for Self-Organising: An Exploratory Study of Timebanks in Finland and in the UK
2018
The article examines the challenges to self-organisation and upscaling of alternative economies from the viewpoint of defending and negotiating social space. Timebanks in Finland and the UK are presented as examples, analysing the difference of defending such social space in the contexts of a traditional welfare state (in the case of Finland) and an austerity-driven government with a “Big Society” ideology (in the case of UK). Both systems of government present different kinds of pressures on timebanks, pushing them to a given ontological categories and to action in accordance with pre-defined political goals. This difference, along with timebank reactions and the question of prospects of o…
Social Network Analysis and Qualitative Interviews for Assessing Geographic Characteristics of Tourism Business Networks.
2015
This study integrates quantitative social network analysis (SNA) and qualitative interviews for understanding tourism business links in isolated communities through analysing spatial characteristics. Two case studies are used, the Surselva-Gotthard region in the Swiss Alps and Longyearbyen in the Arctic archipelago of Svalbard, to test the spatial characteristics of physical proximity, isolation, and smallness for understanding tourism business links. In the larger Surselva-Gotthard region, we found a strong relationship between geographic separation of the three communities on compartmentalization of the collaboration network. A small set of businesses played a central role in steering col…