Search results for "teeri"
showing 10 items of 308 documents
Prevalence of genetically similar Flavobacterium columnare phages across aquaculture environments reveals a strong potential for pathogen control
2022
Intensive aquaculture conditions expose fish to bacterial infections, leading to significant financial losses, extensive antibiotic use and risk of antibiotic resistance in target bacteria. Flavobacterium columnare causes columnaris disease in aquaculture worldwide. To develop a bacteriophage-based control of columnaris disease, we isolated and characterized 126 F. columnare strains and 63 phages against F. columnare from Finland and Sweden in 2017. Bacterial isolates were virulent on rainbow trout (Oncorhynchus mykiss) and fell into four previously described genetic groups A, C, E and G, with genetic groups C and E being the most virulent. Phage host range studied against a collection of 2…
Complete Genome Sequence of Fish Pathogen Flavobacterium columnare Strain B185, Originating from Finland
2019
We report a complete genome sequence of a Finnish isolate of the fish pathogen Flavobacterium columnare. Using PacBio RS II sequencing technology, the complete circular genome of F. columnare strain B185 with 3,261,404 bp was obtained.
Stochastic multicriteria evaluation of district heating systems considering the uncertainties
2018
It is of great importance to choose a suitable district heating (DH) system for a specific DH area from the economics, environment and energy (3E) points of view. This is a multicriteria decision making problem, in which the criteria performance values (PVs) and weighting are characterized by uncertain or imprecise information. In this study, seven candidate DH systems are evaluated from the viewpoints of 3E by the stochastic multicriteria acceptability analysis (SMAA) method. SMAA is able to handle the uncertainties of the criteria PVs and the weighting at the same time. These uncertainties are very common and typical in real-life, but in most cases are not treated judiciously or just negl…
G2/M checkpoint regulation and apoptosis facilitate the nuclear egress of parvoviral capsids
2022
The nuclear export factor CRM1-mediated pathway is known to be important for the nuclear egress of progeny parvovirus capsids in the host cells with virus-mediated cell cycle arrest at G2/M. However, it is still unclear whether this is the only pathway by which capsids exit the nucleus. Our studies show that the nuclear egress of DNA-containing full canine parvovirus. capsids was reduced but not fully inhibited when CRM1-mediated nuclear export was prevented by leptomycin B. This suggests that canine parvovirus capsids might use additional routes for nuclear escape. This hypothesis was further supported by our findings that nuclear envelope (NE) permeability was increased at the late stages…
On the Assessment of Fitness to Drive: Steering and Brake Operative Forces
2021
[EN] The Directive (EU) 2015/653 aimed at facilitating that the maximum force that any disabled driver could make on the vehicle's primary controls could be adjusted to their needs. The technical adjustment in the vehicle's design requires a measurement of the operational forces applied by the driver on the steering and brake controls, in order to determine its functional capacity during the execution of driving maneuvers. The objective of this paper is to define the steering and braking operative forces used for driving current market M1 motor vehicles for the ¿fitness to drive assessment of drivers with physical disabilities. A total of 200 trials were performed with 17 different vehicles…
Vertical stratification of bacteria and archaea in sediments of a small boreal humic lake
2019
ABSTRACT Although sediments of small boreal humic lakes are important carbon stores and greenhouse gas sources, the composition and structuring mechanisms of their microbial communities have remained understudied. We analyzed the vertical profiles of microbial biomass indicators (PLFAs, DNA and RNA) and the bacterial and archaeal community composition (sequencing of 16S rRNA gene amplicons and qPCR of mcrA) in sediment cores collected from a typical small boreal lake. While microbial biomass decreased with sediment depth, viable microbes (RNA and PLFA) were present all through the profiles. The vertical stratification patterns of the bacterial and archaeal communities resembled those in mar…
The effect of out-of-home activity intervention delivered by volunteers on depressive symptoms among older people with severe mobility limitations: a…
2015
Objectives: To examine the effects of an individualized outdoor activity intervention carried out by volunteers on depressive symptoms among community-living older people with severe mobility limitations who have difficulties accessing the outdoors independently. Methods: Secondary analyses of the “Volunteering, Access to Outdoor Activities and Wellbeing in Older People” (VOW) data (ISRCTN56847832). VOW was a randomized single blinded two-arm controlled trial conducted in Jyväskylä, Finland, in 2009-2011. At baseline, 121 people aged 67-92 years with severe mobility limitations were interviewed at home and randomized into either an intervention or waiting list control group. Volunteers (n=4…
Rich resource environment of fish farms facilitates phenotypic variation and virulence in an opportunistic fish pathogen
2020
Phenotypic variation is suggested to facilitate the persistence of environmentally growing pathogens under environmental change. Here, we hypothesized that the intensive farming environment induces higher phenotypic variation in microbial pathogens than natural environment, because of high stochasticity for growth and stronger survival selection compared to the natural environment. We tested the hypothesis with an opportunistic fish pathogen Flavobacterium columnare isolated either from fish farms or from natural waters. We measured growth parameters of two morphotypes from all isolates in different resource concentrations and two temperatures relevant for the occurrence of disease epidemic…
Optogenetic Control of Bacterial Expression by Red Light
2022
In optogenetics, as in nature, sensory photoreceptors serve to control cellular processes by light. Bacteriophytochrome (BphP) photoreceptors sense red and far-red light via a biliverdin chromophore and, in response, cycle between the spectroscopically, structurally, and functionally distinct Pr and Pfr states. BphPs commonly belong to two-component systems that control the phosphorylation of cognate response regulators and downstream gene expression through histidine kinase modules. We recently demonstrated that the paradigm BphP from Deinococcus radiodurans exclusively acts as a phosphatase but that its photosensory module can control the histidine kinase activity of homologous receptors.…
H2 hitting on graphene supported palladium cluster: molecular dynamics simulations
2016
Dissociative adsorption of impinging gas-phase molecular hydrogen (H2) on a palladium cluster (Pd4) supported on defective graphene (C47) was studied by periodic density functional theory molecular dynamics simulations. The H2 on Pd4/C47 collision dynamics were investigated without any particular constraint, except for the Born–Oppenheimer approximation. The study, which had mostly method-testing aims, provided, anyway, valuable information about the collision kinetics of gas-phase molecular hydrogen. This was treated as an impacting projectile having different kinetic energy values. At lower kinetic energies, sticking was ruled by steering effects imputable to the Pd cluster that easily re…