Search results for "terce"
showing 10 items of 394 documents
Efecto de supresión y mediación en el contexto de la intervención psicosocial: Diferencias, similitudes y ejemplos
2013
ResumenEl presente trabajo compara el efecto mediador y el supresor de una tercera variable. Ambos efectos parcelan la varianza de la variable dependiente (Y). La mediación es la reducción, tras introducir una tercera variable (Z) en el modelo, de la proporción de varianza explicada directamente por la variable independiente (X) en la variable criterio (Y) y la supresión es el incremento de la proporción de varianza explicada directamente por la independiente en la variable criterio, al incluir la tercera variable en el modelo. Se analizan las características y diferencias de cada efecto y se examina en qué situaciones se produce uno u otro. Cada situación, compuesta de tres variables corre…
Ecohydrological modelling of flow duration curve in Mediterranean river basins
2013
Abstract Flow duration curve provides an important synthesis of the relevant hydrological processes occurring at the basin scale, and, although it is typically obtained from field observations, different theoretical approaches finalized to its indirect reconstruction have been developed in recent years. In this study a recent ecohydrological model for the probabilistic characterization of base flows is tested through its application to a study catchment located in southern Italy, where long historical series of daily streamflow are available. The model, coupling soil moisture balance with a simplified scheme of the hydrological response of the basin, provides the daily flow duration curve. …
Cell Systems Bioelectricity: How Different Intercellular Gap Junctions Could Regionalize a Multicellular Aggregate
2021
Simple Summary Electric potential patterns across tissues are instructive for development, regeneration, and tumorigenesis because they can influence transcription, migration, and differentiation through biochemical and biomechanical downstream processes. Determining the origins of the spatial domains of distinct potential, which in turn decide anatomical features such as limbs, eyes, brain, and heart, is critical to a mature understanding of how bioelectric signaling drives morphogenesis. We studied theoretically how connexin proteins with different voltage-gated gap junction conductances can maintain multicellular regions at distinct membrane potentials. We analyzed a minimal model that i…
Los Gs auxiliares del imperio
2005
Itraconazole inhibits nuclear delivery of extracellular vesicle cargo by disrupting the entry of late endosomes into the nucleoplasmic reticulum
2021
ABSTRACT Extracellular vesicles (EVs) are mediators of intercellular communication under both healthy and pathological conditions, including the induction of pro‐metastatic traits, but it is not yet known how and where functional cargoes of EVs are delivered to their targets in host cell compartments. We have described that after endocytosis, EVs reach Rab7+ late endosomes and a fraction of these enter the nucleoplasmic reticulum and transport EV biomaterials to the host cell nucleoplasm. Their entry therein and docking to outer nuclear membrane occur through a tripartite complex formed by the proteins VAP‐A, ORP3 and Rab7 (VOR complex). Here, we report that the antifungal compound itracona…
β2 integrin phosphorylation on Thr758 acts as a molecular switch to regulate 14-3-3 and filamin binding
2008
AbstractLeukocyte integrins of the β2 family are essential for immune cell-cell adhesion. In activated cells, β2 integrins are phosphorylated on the cytoplasmic Thr758, leading to 14-3-3 protein recruitment to the β2 integrin. The mutation of this phosphorylation site impairs cell adhesion, actin reorganization, and cell spreading. Thr758 is contained in a Thr triplet of β2 that also mediates binding to filamin. Here, we investigated the binding of filamin, talin, and 14-3-3 proteins to phosphorylated and unphosphorylated β2 integrins by biochemical methods and x-ray crystallography. 14-3-3 proteins bound only to the phosphorylated integrin cytoplasmic peptide, with a high affinity (Kd, 261…
Identification of a copper chaperone from tomato fruits infected with Botrytis cinerea by differential display
2003
Differential display was used to isolate tomato genes responding to fungal infection. Here we describe the isolation and characterization of a gene that is down-regulated in tomato fruits infected with the phytopathogen Botrytis cinerea. The cDNA identified encodes a protein that shares sequence similarity to the amino terminal region of CCH, a copper chaperone from Arabidopsis thaliana, that participates in intracellular copper homeostasis by delivering Cu to the secretory pathway. The fact that this newly characterized tomato gene, referred to as LeCCH (Lycopersicon esculentum copper chaperone), be differentially expressed after fungal infection, suggests an interesting relationship betwe…
Regulatory T Cells and IL-10 Independently Counterregulate Cytotoxic T Lymphocyte Responses Induced by Transcutaneous Immunization
2011
Background: The imidazoquinoline derivate imiquimod induces inflammatory responses and protection against transplanted tumors when applied to the skin in combination with a cognate peptide epitope (transcutaneous immunization, TCI). Here we investigated the role of regulatory T cells (Treg) and the suppressive cytokine IL-10 in restricting TCI-induced cytotoxic T lymphocyte (CTL) responses. Methodology/Principal Findings: TCI was performed with an ointment containing the TLR7 agonist imiquimod and a CTL epitope was applied to the depilated back skin of C57BL/6 mice. Using specific antibodies and FoxP3-diphteria toxin receptor transgenic (DEREG) mice, we interrogated inhibiting factors after…
An analytical model to evaluate the radiated power spectrum of a multipactor discharge in a parallel-plate region
2008
This paper is aimed at studying the electromagnetic radiation pattern of a multipactor discharge occurring in a parallel-plate waveguide. The proposed method is based on the Fourier expansion of the multipactor current in terms of time-harmonic currents radiating in the parallel-plate region. Classical radiation theory combined with the frequency domain Green's function of the problem allows the calculation of both the electric and the magnetic radiated fields. A novel analytical formula for the total radiated power of each multipactor harmonic has been derived. This formula is suitable for predicting multipactor with the third-harmonic technique. The proposed formulation has been successfu…