Search results for "toxin"
showing 10 items of 1434 documents
ACELLULAR PERTUSSIS VACCINE COMPOSED OF GENETICALLY INACTIVATED PERTUSSIS TOXIN: SAFETY AND IMMUNOGENICITY IN 12- TO 24- AND 2-TO 4-MONTH-OLD CHILDREN
1992
To determine whether a nontoxic derivative of pertussis toxin obtained by recombinant DNA technology, PT-9K/129G, is a good candidate for a new pertussis vaccine, we examined the safety and the immunogenicity in children of a vaccine containing 15 micrograms of PT-9K/129G protein and 0.5 mg of aluminum hydroxide per dose. Fifty-three children 12 to 24 months of age and 21 infants aged 2 to 4 months were injected with two and three doses, respectively. The vaccine did not induce significant local or systemic reactions and elicited an increase of antibody titer in more than 98% of the children. The geometric mean of the toxin-neutralizing titers increased after each dose and was 85 units in c…
An ITS-RFLP method to identify black Aspergillus isolates responsible for OTA contamination in grapes and wine
2006
Ochratoxigenic mycobiota in grapes from representative wine regions in Valencia was identified. Black aspergilli were predominant among the different Aspergillus spp. isolated. Restriction digestion analysis of the ITS products was tested as a rapid method to identify isolates of black Aspergillus species from grapes. Restriction endonuclease digestion of the ITS products using the endonucleases HhaI, NlaIII and RsaI, distinguished five types of restriction fragment length polymorphism (RFLP) corresponding to Aspergillus niger, Aspergillus tubingensis, Aspergillus carbonarius and Aspergillus aculeatus species. In addition, a new RFLP type in the A. niger aggregate was identified. The fragme…
Comparison of different sample treatments for the analysis of ochratoxin A in must, wine and beer by liquid chromatography.
2004
Abstract Ochratoxin A (OTA) is a mycotoxin produced by some species of Aspergillus and Penicillium verrucosum. It has been found in foods and feed all over the world. There is a great concern about OTA because it is nephrotoxic and probably, carcinogenic to humans. Most of analytical methods developed for OTA in wine, beer and other products are based on LC with fluorescence detection (LC–FLD). In the present work, various procedures for extraction and/or clean-up for determination of OTA in musts, wine and beer by LC–FLD were compared: (1) dilution with polyethylen glycol 8000 and NaHCO3 solution and clean-up an on immunoaffinity column (IAC); (2) extraction with chloroform and IAC clean-u…
Concentration of ochratoxin A in wines from supermarkets and stores of Valencian Community (Spain)
2004
Ochratoxin A (OTA) is a mycotoxin produced by fungi species belonging to the genera Aspergillus and Penicillium being isolated in alcoholic beverages. The aim of this work is developed and applied a procedure for the analysis of OTA in wines. An analytical method based on immunoaffinity column (IAC) for clean-up, liquid chromatography with fluorescence detection (LC-FD), and LC-FD after of OTA methylation was used to determine the occurrence of OTA in wines. Recoveries of this mycotoxin spiked to red wines at 0.5 ng/ml level were >90% with an average of relative standards deviations of 4%. Furthermore, 116 wine samples from designation of origin (DO) and three samples from food stores of Va…
Effect of ethanol on the ability of Oenococcus oeni to remove ochratoxin A in synthetic wine-like media
2010
The study focused on the changes in bacterial population, malolactic fermentation and ochratoxin A (OTA) levels in cultures of Oenococcus oeni performed in synthetic medium supplemented with ethanol and OTA. Growth was better in cultures containing 5% ethanol and was not observed in cultures containing 15% ethanol. The OTA removal ability of O. oeni depended on ethanol and initial OTA levels. The highest toxin removal percentage was observed in cultures containing 5% ethanol and 2 μg OTA/l. In ethanol-containing medium part of OTA was not sorbed by O. oeni and remained in the liquid medium. Thus, it cannot efficiently eliminate OTA in acidic ethanol-containing beverages, such as wine. The e…
Exposure assessment to ochratoxin A from the consumption of Italian and Hungarian wines
2005
Abstract A total of 267 wine samples including 19 dessert, 186 red, 11 rose and 51 white produced mostly in the years 1997–2002 in Italian and Hungarian regions were analyzed for ochratoxin A (OTA) using inmunoaffinity column (IAC) clean-up and HPLC with fluorimetric detection. None of Hungarian wine samples were contaminated with this mycotoxin. For Italian red wines, 84% of the samples were positive for OTA ranged from 0.01 to 4.00 ng/mL. Furthermore, OTA was detected in 63% of dessert, in 56% of rose and in 19% of white wine samples ranged from 0.01 to 1.64, from 0.01 to 1.04 and from 0.01 to 0.21 ng/mL, respectively. A study of OTA daily exposure assessment in Italian wines was also car…
Lactic acid bacteria: a potential tool to reduce ochratoxin A in wine
2009
E. M. Mateo, A. Medina, F. Mateo, F.M. Valle Algarra, R. Mateo, and M. Jimenez 1 Dpto. de Microbiologia i Ecologia. Universitat de Valencia. Dr. Moliner 50, E-46100, Burjassot, Valencia, Spain. 2 Dpto. de Quimica Analitica. Universitat de Valencia. Dr. Moliner 50, E-46100, Burjassot, Valencia, Spain. 3 Dpto. de Ingenieria Electronica, Universitat Politecnica de Valencia, Camino de Vera, 14. E-46022, Valencia, Spain.
Killer toxin-secreting double-stranded RNA mycoviruses in the yeasts Hanseniaspora uvarum and Zygosaccharomyces bailii.
1994
Killer toxin-secreting strains of the yeasts Hanseniaspora uvarum and Zygosaccharomyces bailii were shown to contain linear double-stranded RNAs (dsRNAs) that persist within the cytoplasm of the infected host cell as encapsidated virus-like particles. In both yeasts, L- and M-dsRNAs were associated with 85-kDa major capsid protein, whereas the additional Z-dsRNA (2.8 kb), present only in the wild-type Z. bailii killer strain, was capsid protein, whereas the additional Z-dsRNA (2.8 kb), present only in the wild-type Z. bailii killer strain, was shown to be encapsidated by a 35-kDa coat protein. Although Northern (RNA) blot hybridizations indicated that L-dsRNA from Z. bailii is a LA species,…
Ecological role of mycotoxins in wheat crop residues and consequences on the multitrophic interactions in the soil and further development of Fusariu…
2009
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Le désherbage d’automne contre l’ergot du seigle les traitements herbicides d’automne, un levier chimique efficace de lutte contre l’ergot du seigle
2013
Context and study: Trials in field allowed to show a link between a good grass weeding (blackgrass and rye grass) and extent of ergot contaminations. Makhteshim Agan France and Arvalis tested on 15 trials in 2011-2012, 2 ureas, as isoproturon against blackgrass, alone or in pro-gram with a sulfonylurea, to evaluate their indirect effect against ergot. Field trials were set up on sensitive crops to ergot (rye, wheat and triticale) infested with artificial ergot contaminations and weed seeds sensible to ALS. Contaminations were evaluated first in field on weeds and on crop, and then on harvest, by assessing sclerotia and alkaloids content in harvested samples. results: For all evaluation mean…