Search results for "uci"

showing 10 items of 5317 documents

Recombinant NeutraLite Avidin: a non-glycosylated, acidic mutant of chicken avidin that exhibits high affinity for biotin and low non-specific bindin…

2000

AbstractA recombinant non-glycosylated and acidic form of avidin was designed and expressed in soluble form in baculovirus-infected insect cells. The mutations were based on the same principles that guided the design of the chemically and enzymatically modified avidin derivative, known as NeutraLite Avidin. In this novel recombinant avidin derivative, five out of the eight arginine residues were replaced with neutral amino acids, and two of the lysine residues were replaced by glutamic acid. In addition, the carbohydrate-bearing asparagine-17 residue was altered to an isoleucine, according to the known sequences of avidin-related genes. The resultant mutant protein, termed recombinant Neutr…

StreptavidinGlycosylationMolecular Sequence DataBiophysicsBiotinChick EmbryoNon-specific bindingBiochemistrylaw.inventionchemistry.chemical_compoundBiotinstomatognathic systemStructural BiologylawMutant proteinNon-glycosylated mutantGeneticsAnimalsHumansAmino Acid SequenceIsoelectric PointProtein Structure QuaternaryMolecular BiologyCells CulturedbiologyAvidin-biotin technologyDNACell BiologyProtein engineeringrespiratory systemAvidinRecombinant ProteinsKineticsAmino Acid SubstitutionchemistryBiochemistryBiotinylationMutationbiology.proteinRecombinant DNAThermodynamicsProtein engineeringEndopeptidase KIsoleucineBaculoviridaeProtein BindingAvidinFEBS Letters
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Long Non Coding RNA H19: A New Player in Hypoxia-Induced Multiple Myeloma Cell Dissemination

2019

The long non-coding RNA H19 (lncH19) is broadly transcribed in the first stage of development and silenced in most cells of an adult organism

Stromal cellCellHIF-1αBiologyModels BiologicalCatalysisArticleInorganic Chemistrylcsh:ChemistryChemokine receptorWestern blotSettore BIO/13 - Biologia ApplicataCell Line TumormedicineCell AdhesionGene silencingHumansHIF-1 alfaPhysical and Theoretical ChemistryMolecular Biologylcsh:QH301-705.5Spectroscopymedicine.diagnostic_testCell growthhypoxiaOrganic ChemistryGeneral MedicineHypoxia (medical)Hypoxia-Inducible Factor 1 alpha Subunitfemale genital diseases and pregnancy complicationsComputer Science ApplicationsCell biologyGene Expression Regulation Neoplasticmultiple myelomamedicine.anatomical_structurelcsh:Biology (General)lcsh:QD1-999Cell cultureembryonic structuresRNA Long Noncodingmedicine.symptomStromal Cellslong non-coding RNA H19 (lncH19)International Journal of Molecular Sciences
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REGULATORY ELEMENTS OF THE LEUKAEMIA INHIBITORY FACTOR (LIF) PROMOTER IN MURINE BONE MARROW STROMAL CELLS

1999

Leukaemia inhibitory factor (LIF) plays an important role as a haematopoietically active cytokine. As described earlier in a murine model, interleukin 1 (IL-1) induced LIF mRNA and protein expression. We utilized the murine cell line +/+-1.LDA11 to further define regulatory mechanisms of LIF expression in bone marrow stromal cells. The production of LIF mRNA is stimulated by IL-1beta, TNF-alpha, and the cAMP analogue 8-bromoadenosine 3':5'-monophosphate (8BrcAMP). LIF mRNA expression is controlled at the transcriptional level. Different fragments from -542 to -45 bp 5' upstream of the transcriptional start site of the murine LIF gene were fused to the luciferase gene. All LIF-promoter lucif…

Stromal cellRecombinant Fusion Proteinsmedicine.medical_treatmentImmunology8-Bromo Cyclic Adenosine MonophosphateBone Marrow CellsStimulationRegulatory Sequences Nucleic AcidBiologyLeukemia Inhibitory FactorBiochemistryMiceGenes ReportermedicineAnimalsHumansImmunology and AllergyLuciferaseRNA MessengerNuclear proteinPromoter Regions GeneticMolecular BiologyCells CulturedLymphokinesMessenger RNAInterleukin-6Tumor Necrosis Factor-alphaInterleukinHematologyMolecular biologyGrowth InhibitorsRecombinant ProteinsCytokinemedicine.anatomical_structureGene Expression RegulationBone marrowStromal CellsInterleukin-1Cytokine
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Reproducibility of trace element time-series (Na/Ca, Mg/Ca, Mn/Ca, Sr/Ca, and Ba/Ca) within and between specimens of the bivalve Arctica islandica – …

2017

Abstract Trace element time-series in bivalve mollusk shells and other (biogenic) materials can potentially serve as environmental proxies. Yet, the applicability of element-to-calcium ratios is often challenging, because non-environmental factors such as vital effects distort or mask environmental signals. If a trace element time-series is driven by an environmental factor, it should be reproducible within and between coeval specimens of the same species. In the present study, we tested whether time-series of trace element-to-calcium ratios can be reproduced within and between coeval specimens of the bivalve Arctica islandica and thus whether an external signal is encoded in the temporal v…

StrontiumReproducibility010504 meteorology & atmospheric sciencesbiologyMagnesiumSodiumTrace elementPaleontologychemistry.chemical_elementMineralogyBariumManganese010502 geochemistry & geophysicsOceanographybiology.organism_classification01 natural scienceschemistryArctica islandicaEcology Evolution Behavior and SystematicsGeology0105 earth and related environmental sciencesEarth-Surface ProcessesPalaeogeography, Palaeoclimatology, Palaeoecology
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Workplace-based assessment of communication skills: A pilot project addressing feasibility, acceptance and reliability

2016

Background: Imparting communication skills has been given great importance in medical curricula. In addition to standardized assessments, students should communicate with real patients in actual clinical situations during workplace-based assessments and receive structured feedback on their performance. The aim of this project was to pilot a formative testing method for workplace-based assessment. Our investigation centered in particular on whether or not physicians view the method as feasible and how high acceptance is among students. In addition, we assessed the reliability of the method. Method: As part of the project, 16 students held two consultations each with chronically ill patients …

Students Medical020205 medical informaticsformative assessmenteducationlcsh:MedicinePilot Projects02 engineering and technologyarbeitsplatzbasierte PrüfungArticle03 medical and health sciences0302 clinical medicine0202 electrical engineering electronic engineering information engineeringHumans030212 general & internal medicineWorkplacelcsh:LC8-6691Education Medicallcsh:Special aspects of educationCommunicationformative Prüfunglcsh:RReproducibility of Resultsworkplace based assessment610 Medical sciences; MedicineBerlinKommunikative KompetenzcompetencyClinical CompetenceGMS Journal for Medical Education
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The role of local and regional authorities in the implementation of the european social charter

2018

URL del artículo en la web de la Revista: https://www.upo.es/revistas/index.php/lex_social/article/view/3475/2749 Le présent article met l¿accent sur le fonctionnement et les avantages des deux mécanismes de contrôle de la Charte sociale européenne (système de rapports et procédure de réclamations collectives) en termes de progrès réalisés par les États Parties dans le domaine des droits sociaux avec une action primordiale des collectivités locales et régionales, permettant ainsi de forger une conscience européenne plus solide « par le bas ». En ce sens, dans le cadre du « Processus de Turin » pour la Charte sociale européenne, les collectivités territoriales peuvent exercer une influence p…

Subsidiarity Turin processSocial rightsRevised European Social CharterMultilevel protectionDret constitucional
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Models dinàmics de competició entre llengües, aplicats al cas català-castellà, al País Valencià

2015

Antecedents: l'any 2003 Abrams i Strogatz van publicar un article en la revista Nature, sobre models dinàmics de la desaparició de les llengües. Dos anys més tard Mira i Paredes van esmenar el model proposat pels autors anteriors. Mètode: en aquest article es presenta una recerca inicial de la dinàmica de l'evolució del procés de substitució lingüística al País Valencià a partir de models matemàtics d'equacions diferencials. En particular, discutim l'aplicació dels models esmentats anteriorment a dades empíriques obtingudes d'enquestes realitzades pel Servici d'Investigació i Estudis Sociolingüístics (SIES) de la Generalitat Valenciana i per l'Acadèmia Valenciana de la Llengua (AVL). En el …

Substitució lingüística País Valencià llengua catalana. Artículo:PSICOLOGÍA [UNESCO]UNESCO::PSICOLOGÍA
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Sulphate-reducing bacteria in paper machine waters and in suction roll perforations

1978

To define some aspects of the biological corrosion sulphate-reducing bacteria were studied in paper machine waters and in plugged perforations of a suction roll. The desulphuricants were most active on passive fiber recipients. Most bacteria found in fiber plugs taken from the perforations of suction rolls belonged to the genus Desulfovibrio. Desulphuricants were found mainly at the outer ends of plugged perforations, where corrosion of the roll metal is most evident.

Suction (medicine)Materials sciencebusiness.product_categorybiologyPerforation (oil well)General Medicinebiology.organism_classificationApplied Microbiology and BiotechnologyDesulfovibrioMicrobiologyCorrosionPaper machineGenus DesulfovibrioSulfate-reducing bacteriaComposite materialbusinessBiotechnologyEuropean Journal of Applied Microbiology and Biotechnology
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Dificultades para Codificar, Relacionar y Categorizar Problemas Verbales Algebraicos: dos estudios con estudiantes de secundaria y profesores en form…

2014

En resolución de problemas verbales por transferencia, la activación de problemas ya conocidos que sirvan de guía, depende de las analogías percibidas entre éstos y el problema a resolver. Se desarrollan dos estudios relacionados para analizar en qué características se basan los estudiantes para codificar problemas y detectar sus analogías, en tareas de categorización (sorting). Se utilizaron técnicas cuantitativas y cualitativas combinadas. Primero se analizó cómo los estudiantes de secundaria son influidos por diferentes variables características de problemas de ciencias. Una gran proporción de sujetos no fue capaz de percibir las analogías y diferencias adecuadas entre problemas. El segu…

Superficie y Estructura de problemasFamiliaridad con la temáticaFuncionesProblem Surface and StructureSortingProfessors d'educació secundàriaInicialAnalogías entre ProblemasAnalogies among ProblemsSolució de problemesEducationFocus (linguistics)Task (project management)Ciència EnsenyamentDificultadesMathematics (miscellaneous)Tipos de problemasAlgebraic Verbal Problem SolvingMathematics educationCategorización de problemasFamiliarity with the subjetcEntrevistasResolución de Problemas Verbales AlgebraicosProblem sorting
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Superresolución por apertura sintética en holografía digital sin lentes. Aplicaciones a microscopía coherente.

2016

EL presente trabajo trata sobre técnicas de mejora de la resolución por apertura sintética en aquellos sistemas donde ésta,se ve limitada por la propia naturaleza de la luz (resolución limitada por la difracción), considerando en todos los casos que ni la resolución geométrica, ni la resolución asociada al ruido constituyen un factor limitante en el proceso de obtención de las imágenes. Además, se analizan diferentes técnicas que permiten aumentar dichos límites impuestos por la difracción sin necesidad de modificar las propiedades de los elementos que constituyen el sistema. Estas técnicas, a las que denominaremos técnicas de superresolución por apertura sintética se aplicarán a sistemas d…

Superresolución:FÍSICA::Óptica ::Microscopios [UNESCO]:FÍSICA::Óptica ::Holografía [UNESCO]UNESCO::FÍSICA::Óptica ::HolografíaApertura SintéticaUNESCO::FÍSICA::Óptica ::MicroscopiosHolografía
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