Search results for "uva"
showing 10 items of 2916 documents
Induction of cholesterol biosynthesis by archazolid B in T24 bladder cancer cells.
2014
Abstract Background Resistance of cancer cells towards chemotherapeutics represents a major cause of therapy failure. The objective of our study was to evaluate cellular defense strategies in response to the novel vacuolar H+-ATPase inhibitor, archazolid B. Experimental approach: The effects of archazolid B on T24 bladder carcinoma cells were investigated by combining “omics” technologies (transcriptomics (mRNA and miRNA) and proteomics). Free cholesterol distribution was determined by filipin staining using flow cytometry and fluorescence microscopy. Flow cytometry was performed for LDLR surface expression studies. Uptake of LDL cholesterol was visualized by confocal microscopy. SREBP acti…
Fluvastatin stabilizes the blood–brain barrier in vitro by nitric oxide-dependent dephosphorylation of myosin light chains
2006
Inhibition of the 3-hydroxy-3-methylglutaryl-coenzyme-A reductase and the downstream mevalonate pathway is in part responsible for the beneficial effects that statins exert on the cardiovascular system. In this study we aimed at analysing the stabilizing effects of fluvastatin on the blood-brain barrier (BBB) integrity, using an in vitro co-culture model of ECV304 and C6, or primary bovine endothelial cells and rat astrocytes. Fluvastatin dose-dependently (1-25 micromol/l) increased barrier integrity as analysed by measurements of transendothelial electrical resistance (TEER). This effect (117.4+/-2.6% at 25 micromol/l) was significantly reduced by the nitric oxide (NO) synthase inhibitor L…
Fluvastatin prevents glutamate-induced blood-brain-barrier disruption in vitro.
2008
Abstract Glutamate is an important excitatory amino acid in the central nervous system. Under pathological conditions glutamate levels dramatically increase. Aim of the present study was to examine whether the HMG-CoA inhibitor fluvastatin prevents glutamate-induced blood-brain-barrier (BBB) disruption. Measurements of transendothelial electrical resistance (TEER) were performed to analyze BBB integrity in an in vitro co-culture model of brain endothelial and glial cells. Myosin light chain (MLC) phosphorylation was detected by immunohistochemistry, or using the in-cell western technique. Intracellular Ca 2+ and reactive oxygen species (ROS) levels were analyzed using the fluorescence dyes …
Updated insights into the mechanism of action and clinical profile of the immunoadjuvant QS-21: A review
2019
Background Vaccine adjuvants are compounds that significantly enhance/prolong the immune response to a co-administered antigen. The limitations of the use of aluminium salts that are unable to elicite cell responses against intracellular pathogens such as those causing malaria, tuberculosis, or AIDS, have driven the development of new alternative adjuvants such as QS-21, a triterpene saponin purified from Quillaja saponaria. Purpose The aim of this review is to attempt to clarify the mechanism of action of QS-21 through either receptors or signaling pathways in vitro and in vivo with special emphasis on the co-administration with other immunostimulants in new adjuvant formulations, called a…
Transforming adult education from neo-liberal to holistically inclusive adult education in Baltic States
2021
In this chapter, we explore the data of nation-wide adult education programmes in three Baltic states. These programmes incorporate informal learning elements from the perspective of active participatory citizenship (APC) and, therefore, this provision aims to enhance opportunities for young adults in vulnerable positions. We posit that the concept of active participatory citizenship (EduMAP Concept Note 2017) that aims at developing young adults’ politico-legal, socio-cultural and socio-economic proactiveness could be used for illustrating these educational programmes from the holistic education aspects (Jarvis and Parker 2005). Holistic approach to adult education (AE) denotes that knowle…
Inovacijos ir bendrovės vertė: Baltijos šalių patirtis
2021
Inovacijos yra konkurencingumo pagrindas. Kartu pagrindinis vadovo uždavinys yra savininkų turto maksimizavimas, todėl svarbu tyrinėti inovacijų ir bendrovės vertės santykį. Šio tyrimo tikslas – ištirti inovacijų poveikį organizacijos vertei. Tiriamos Baltijos šalių bendrovės, veikusios 2005–2011 metų laikotarpiu. Tyrimas parodė, kad inovatyvių bendrovių vertė yra didesnė, didesnės ir jos pačios. Nustatyta, kad didesnė bendrovės vertė pasiekiama, jei nematerialių išteklių dalis viršija 1 %. Taikant koreliaciją ir regresinę analizę, nustatyta, kad inovacijos yra svarbi organizacijos vertės determinantė, organizacijos dydis yra silpnesnis veiksnys, o augimas – pats silpniausias veiksnys. Stra…
Target vs non-target analysis to determine pesticide residues in fruits from Saudi Arabia and influence in potential risk associated with exposure
2017
The occurrence of pesticide residues in fruits was determined by a target method for 62 analytes and a wide scope screening method against a database of 500 pesticides. Limits of quantification (LOQs) were from 0.5 to 6.3 μg kg−1 for the target method and from 0.5 to 119 μg kg−1 for the non-target. Thirty samples: dates, apples, oranges, tangerines, lemons and grapefruits were selected because their high consumption, except lemons and grapefruits that were to cover all citrus fruits. Using the target method, 15 compounds (mostly insecticides and fungicides) out of 62 pesticides (organophosphorus, carbamates, pyrethroids, chloroacetanilides, triazines, triazoles, imidazoles, etc.) were detec…
Interactionist Approach to Visual Aesthetics in HCI
2021
Visual Aesthetics has gathered interest among scholars in HCI research. The growing interest stems from examinations of the aesthetic-usability effect (“what is beautiful is usable”), and possibly vice versa. Thus, numerous studies focus on understanding how we make sense and experience visual entities in interacting with technology. However, theoretical, and methodological stances vary, which impact conclusions of the studies conducted, and thus, affect design implications. Visual experience research in HCI lacks detailed conceptualizations of the constituents of visual experience and understanding of how these conceptualizations affect the overall research results through implicit methodo…
Rotavirus 2/6 virus-like particles administered intranasally in mice, with or without the mucosal adjuvants cholera toxin and Escherichia coli heat-l…
2001
ABSTRACTWe investigated the rotavirus-specific lymphocyte responses induced by intranasal immunization of adult BALB/c mice with rotavirus 2/6 virus-like particles (2/6-VLPs) of the bovine RF strain, by assessing the profile of cytokines produced after in vitro restimulation and serum and fecal antibody responses. The cytokines produced by splenic cells were first evaluated. Intranasal immunization with 50 μg of 2/6-VLPs induced a high serum antibody response, including immunoglobulin G1 (IgG1) and IgG2a, a weak fecal antibody response, and a mixed Th1/Th2-like profile of cytokines characterized by gamma interferon and interleukin 10 (IL-10) production and very low levels of IL-2, IL-4, and…