Search results for "vector"

Is tubulin the sole antigen recognized by a putative anti-bursicon antibody?

1999

Abstract A 56-kDa polypeptide suspected to be the tanning hormone `bursicon' was analyzed using the monoclonal antibody (mAb) 01C10 of Song and Ma. We studied the beetle Tenebrio molitor, for which data on bursicon have been recently published. After purification by two-dimensional gel electrophoresis of brain proteins, the immunoreactive 56-kDa polypeptide was trypsinated and microsequenced. The obtained sequences revealed a high homology with α- and β-tubulins. In a complementary study, immunoreactive clones were isolated, using the 01C10 mAb, from a library in expression vector obtained from Drosophila melanogaster head cDNAs. Again, the isolated clones were found, after cDNA sequencing,…

Assessing Transfer Entropy in cardiovascular and respiratory time series under long-range correlations.

2021

Heart Period (H) results from the activity of several coexisting control mechanisms, involving Systolic Arterial Pressure (S) and Respiration (R), which operate across multiple time scales encompassing not only short-term dynamics but also long-range correlations. In this work, multiscale representation of Transfer Entropy (TE) and of its decomposition in the network of these three interacting processes is obtained by extending the multivariate approach based on linear parametric VAR models to the Vector AutoRegressive Fractionally Integrated (VARFI) framework for Gaussian processes. This approach allows to dissect the different contributions to cardiac dynamics accounting for the simultane…

Persistent episomal transgene expression in liver following delivery of a scaffold/matrix attachment region containing non-viral vector

2008

An ideal gene therapy vector should enable persistent transgene expression without limitations of safety and reproducibility. Here we report the development of a non-viral episomal plasmid DNA (pDNA) vector that appears to fulfil these criteria. This pDNA vector combines a scaffold/matrix attachment region (S/MAR) with a human liver-specific promoter (alpha1-antitrypsin (AAT)) in such a way that long-term expression is enabled in murine liver following hydrodynamic injection. Long-term expression is demonstrated by monitoring the longitudinal luciferase expression profile for up to 6 months by means of in situ bioluminescent imaging. All relevant control pDNA constructs expressing luciferas…

A time domain triangle method approach to estimate actual evapotranspiration: Application in a Mediterranean region using MODIS and MSG-SEVIRI produc…

2016

Abstract In this study, spatially distributed estimates of regional actual evapotranspiration (ET) were obtained using a revised procedure of the so called “triangle method” to parameterize the Priestley–Taylor ϕ coefficient. In the procedure herein proposed, named Time-Domain Triangle Method (TDTM), the triangular feature space was parameterized considering pairs of T s –VI values obtained by exploring, for each pixel, only their temporal dynamics. This new method was developed using time series products provided by MODIS and MSG-SEVIRI sensors. Moreover the proposed procedure does not depend on ancillary data, and it is only based on remotely sensed vegetation indices and day–night time l…

On inductive dimensions for fuzzy topological spaces

1995

An approach to the dimension theory for fuzzy topological spaces is being developed. The appropriate context for this theory is not the category CFT of Chang fuzzy topological spaces or some of its modifications, but the category Hut introduced in the paper (this category is a slight extension of the category H of Hutton fuzzy topological spaces Hutton (1980). The frames of this category allow us to make exposition simple and uniform, and on the other hand to make it applicable in quite a general setting.

On Słowikowski, Raíkov and De Wilde Closed Graph Theorems

1986

Publisher Summary This chapter focuses on the Slowikowski, Raikov and De Wilde closed graph theorems. The vector spaces used in the chapter, are defined over the field Ղ of real or complex numbers. The term, “space” means separated topological vector space, unless the contrary is specifically stated. If Ω is a non-empty open subset of the n -dimensional euclidean space, then the Schwartz space ҟ′(Ω) endowed with the strong topology belongs to this class. The chapter also studies the classes of spaces related with this conjecture. The class of Slowikowski spaces contains the F-spaces and it is stable with respect to the operations that include: countable topological direct sums, closed subsp…

Noetherian type in topological products

2010

The cardinal invariant "Noetherian type" of a topological space $X$ (Nt(X)) was introduced by Peregudov in 1997 to deal with base properties that were studied by the Russian School as early as 1976. We study its behavior in products and box-products of topological spaces. We prove in Section 2: 1) There are spaces $X$ and $Y$ such that $Nt(X \times Y) < \min\{Nt(X), Nt(Y)\}$. 2) In several classes of compact spaces, the Noetherian type is preserved by the operations of forming a square and of passing to a dense subspace. The Noetherian type of the Cantor Cube of weight $\aleph_\omega$ with the countable box topology, $(2^{\aleph_\omega})_\delta$, is shown in Section 3 to be closely related …

Localification of variable-basis topological systems

2011

The paper provides another approach to the notion of variable-basis topological system generalizing the fixed-basis concept of S. Vickers, considers functorial relationships between the categories of modified variable-basis topological systems and variable-basis fuzzy topological spaces in the sense of S.E. Rodabaugh and shows that the procedure of localification is possible in the new setting. Quaestiones Mathematicae 33(2010), 11–33

Ultimate Order Statistics-Based Prototype Reduction Schemes

2013

Published version of a chapter in the book: AI 2013: Advances in Artificial Intelligence. Also available from the publisher at: http://dx.doi.org/10.1007/978-3-319-03680-9_42 The objective of Prototype Reduction Schemes (PRSs) and Border Identification (BI) algorithms is to reduce the number of training vectors, while simultaneously attempting to guarantee that the classifier built on the reduced design set performs as well, or nearly as well, as the classifier built on the original design set. In this paper, we shall push the limit on the field of PRSs to see if we can obtain a classification accuracy comparable to the optimal, by condensing the information in the data set into a single tr…

2004

This paper presents the use of Support Vector Machines (SVMs) for prediction and analysis of antisense oligonucleotide (AO) efficacy. The collected database comprises 315 AO molecules including 68 features each, inducing a problem well-suited to SVMs. The task of feature selection is crucial given the presence of noisy or redundant features, and the well-known problem of the curse of dimensionality. We propose a two-stage strategy to develop an optimal model: (1) feature selection using correlation analysis, mutual information, and SVM-based recursive feature elimination (SVM-RFE), and (2) AO prediction using standard and profiled SVM formulations. A profiled SVM gives different weights to …