Search results for "vectors"
showing 10 items of 601 documents
Malaria resurgence risk in southern Europe: climate assessment in an historically endemic area of rice fields at the Mediterranean shore of Spain
2010
Abstract Background International travel and immigration have been related with an increase of imported malaria cases. This fact and climate change, prolonging the period favouring vector development, require an analysis of the malaria transmission resurgence risk in areas of southern Europe. Such a study is made for the first time in Spain. The Ebro Delta historically endemic area was selected due to its rice field landscape, the presence of only one vector, Anopheles atroparvus, with densities similar to those it presented when malaria was present, in a situation which pronouncedly differs from already assessed potential resurgence areas in other Mediterranean countries, such as France an…
Rapid, reproducible transduction of select forebrain regions by targeted recombinant virus injection into the neonatal mouse brain
2009
Viral vectors can mediate long-term gene expression in different regions of the brain. Recombinant adeno-associated virus (rAAV) and Lenti virus (LV) have both gained prominence due to their ability to achieve specific transduction of various neuronal populations. Whilst widespread gene delivery has been obtained by targeted injection of rAAV in various brain structures, LV has also been utilized for infection of stem cell populations for cell lineage tracing. Both viral vector systems are most commonly used for gene delivery in mature brains, but the great potential of somatic gene delivery into the neonate brain has not been systematically exploited. Here we provide a systematic guideline…
Rotavirus-Specific Cytotoxic T Lymphocytes Recognize Overlapping Epitopes in the Amino-Terminal Region of the VP7 Glycoprotein
1999
Abstract Rotavirus-specific cytotoxic T lymphocytes (CTL) play an important role in the resolution of rotavirus infection. The outer capsid glycoprotein, VP7, elicits a class I MHC-restricted CTL response. Vaccinia virus recombinants expressing the VP7 genes from simian rotavirus SA11 (serotype G3) and from the RF strain of bovine rotavirus (serotype G6) were used to analyze the CTL activity to this antigen in BALB/c (H-2 d ) and C57BL/6 (H-2 b ) mice neonatally infected with homologous and heterologous rotaviruses. A vaccinia virus recombinant expressing the first amino-terminal 88 amino acids of VP7 was constructed and used to search for cross-reactive CTL against this region of the prote…
Novel Glutamate–Putrescine Ligase Activity in Haloferax mediterranei: A New Function for glnA-2 Gene
2021
This article belongs to the Section Cellular Biochemistry.
Invariant Feature Matching for Image Registration Application Based on New Dissimilarity of Spatial Features
2016
An invariant feature matching method is proposed as a spatially invariant feature matching approach. Deformation effects, such as affine and homography, change the local information within the image and can result in ambiguous local information pertaining to image points. New method based on dissimilarity values, which measures the dissimilarity of the features through the path based on Eigenvector properties, is proposed. Evidence shows that existing matching techniques using similarity metrics--such as normalized cross-correlation, squared sum of intensity differences and correlation coefficient--are insufficient for achieving adequate results under different image deformations. Thus, new…
Targeting Homer genes using adeno-associated viral vector: lessons learned from behavioural and neurochemical studies.
2008
Over a decade of in-vitro data support a critical role for members of the Homer family of postsynaptic scaffolding proteins in regulating the functional architecture of glutamate synapses. Earlier studies of Homer knockout mice indicated a necessary role for Homer gene products in normal mesocorticolimbic glutamate transmission and behaviours associated therewith. The advent of adeno-associated viral vectors carrying cDNA for, or short hairpin RNA against, specific Homer isoforms enabled the site-directed targeting of Homers to neurons in the brain. This approach has allowed our groups to address developmental issues associated with conventional knockout mice, to confirm active roles for di…
Functional roles of the membrane-associated AAV protein MAAP
2021
AbstractWith a limited coding capacity of 4.7 kb, adeno-associated virus (AAV) genome has evolved over-lapping genes to maximise the usage of its genome. An example is the recently found ORF in the cap gene, encoding membrane-associated accessory protein (MAAP), located in the same genomic region as the VP1/2 unique domain, but in a different reading frame. This 13 KDa protein, unique to the dependovirus genus, is not homologous to any known protein. Our studies confirm that MAAP translation initiates from the first CTG codon found in the VP1 ORF2. We have further observed MAAP localised in the plasma membrane, in the membranous structures in close proximity to the nucleus and to the nuclea…
Sharp estimates and saturation phenomena for a nonlocal eigenvalue problem
2011
Abstract We determine the shape which minimizes, among domains with given measure, the first eigenvalue of a nonlocal operator consisting of a perturbation of the standard Dirichlet Laplacian by an integral of the unknown function. We show that this problem displays a saturation behaviour in that the corresponding value of the minimal eigenvalue increases with the weight affecting the average up to a (finite) critical value of this weight, and then remains constant. This critical point corresponds to a transition between optimal shapes, from one ball as in the Faber–Krahn inequality to two equal balls.
Distribution of Eigenvalues for Semi-classical Elliptic Operators with Small Random Perturbations, Results and Outline
2019
In this chapter we will state a result asserting that for elliptic semi-classical (pseudo-)differential operators the eigenvalues are distributed according to Weyl’s law “most of the time” in a probabilistic sense. The first three sections are devoted to the formulation of the results and in the last section we give an outline of the proof that will be carried out in Chaps. 16 and 17.
Dimension of self-affine sets for fixed translation vectors
2016
An affine iterated function system is a finite collection of affine invertible contractions and the invariant set associated to the mappings is called self-affine. In 1988, Falconer proved that, for given matrices, the Hausdorff dimension of the self-affine set is the affinity dimension for Lebesgue almost every translation vectors. Similar statement was proven by Jordan, Pollicott, and Simon in 2007 for the dimension of self-affine measures. In this article, we have an orthogonal approach. We introduce a class of self-affine systems in which, given translation vectors, we get the same results for Lebesgue almost all matrices. The proofs rely on Ledrappier-Young theory that was recently ver…