Search results for "vidi"
showing 10 items of 346 documents
Live imaging of developmental processes in a living meristem of Davidia involucrata (Nyssaceae)
2014
Morphogenesis in plants is usually reconstructed by scanning electron microscopy and histology of meristematic structures. These techniques are destructive and require many samples to obtain a consecutive series of states. Unfortunately, using this methodology the absolute timing of growth and complete relative initiation of organs remain obscure. To overcome this limitation, an in vivo observational method based on Epi-Illumination Light Microscopy (ELM) was developed and tested with a male inflorescence meristem (floral unit) of the handkerchief tree Davidia involucrata Baill. (Nyssaceae). We asked whether the most basal flowers of this floral unit arise in a basipetal sequence or, altern…
Tur e Iraj, Caín y Abel: Semejanzas iconográficas en la interminable lucha del mal y el bien.
2015
ABSTRACT: The duality between good and evil is a common topic in all civilizations regardless of chronology or geographical location. It is a conflict reflected in art history, and on the present occasion, through the iconographic types of Cain killing Abel as well as Tur murdering Iraj. The roots of these myths are deeply embedded in two highly relevant written sources, the Bible and the Shahnameh or The Book of Kings . The latter was written by the great Iranian poet Ferdowsi. The legends, despite having different origins, nevertheless have many similarities and they share a particular iconographic type. I analyze each of the myths along with their pictorial representations, which connec…
Double Thermoresponsive Block Copolymers Featuring a Biotin End Group
2010
A poly(oligo(ethylene glycol) monomethyl ether methacrylate)-block-poly(N-isopropyl methacrylamide) (POEGMA-b-PNIPMAM) block copolymer with a biotin end group on the PNIPMAM block as a biotarget was synthesized as a model system for temperature-controlled polymer immobilization. The synthesis was based on RAFT polymerization followed by postpolymerization modification of an activated ester precursor block and an exchange of the dithioester end group within one step. NMR, differential scanning calorimetry (DSC), dynamic light scattering (DLS), and turbidimetry measurements were performed to investigate the stimulus-responsive properties. The double thermoresponsive POEGMA-b-PNIPMAM with biot…
Increasing Functional Avidity of T Cell Receptor (TCR)-Redirected T Cells by Removing Defined N-Glycoslyation Sites in the Constant Domain of Introdu…
2007
Abstract Adoptive transfer of T lymphocytes transduced with a TCR to impart tumor reactivity has been reported as potential strategy to redirect immune responses to target cancer cells. However, the affinities of most TCRs specific for shared tumor antigens that can be isolated are usually low, in part reflecting the nature of the targeted tumor antigens which are self-proteins. Thus strategies that can increase the affinity or functional avidity of TCRs to be used in therapy to transduce T cells might be therapeutically beneficial. However, current strategies for increasing TCR affinity require extensive and usually random mutagenesis followed by screening the many derived mutations, and m…
Qualitative assessment of SARS-CoV-2-specific antibody avidity by lateral flow immunochromatographic IgG/IgM antibody assay.
2020
Abstract Knowledge of the precise timing of SARS‐CoV‐2 infection may be of clinical and epidemiological relevance. The presence of low‐avidity IgGs has conventionally been considered an indicator of recent infection. Here, we carried out qualitative assessment of SARS‐CoV‐2‐specific antibody avidity using an urea (6M) dissociation test performed on a lateral flow immunochromatographic IgG/IgM device. We included a total of 76 serum specimens collected from 57 COVID‐19 patients, of which 39 tested positive for both IgG and IgM and 37 only for IgG. Sera losing IgG reactivity after urea treatment (n = 28) were drawn significantly earlier (P = .04) after onset of symptoms than those which prese…
Early neutralizing and glycoprotein B (gB)-specific antibody responses to human cytomegalovirus (HCMV) in immunocompetent individuals with distinct c…
2000
Abstract Background: Antibodies with functional anti-Human Cytomegalovirus (HCMV) activity are likely to be involved in preventing virus dissemination and thus may contribute to minimize the clinical manifestations of infection. Objectives: To investigate the role of humoral immunity in modulating the clinical expression of primary Human Cytomegalovirus (HCMV) infection in immunocompetent persons. Study design: Neutralizing (NA) and glycoprotein B (gB)-specific antibodies were quantitated in acute-phase and late-convalescence phase sera from 19 individuals who developed either HCMV mononucleosis (12) or oligosymptomatic hepatitis (seven). Results: The levels of NA in sera drawn early after …
First insights into weed communities of rice agrocoenoses in southern Thailand
2015
The study presents the results of geobotanical investigations conducted in rice fields in southern Thailand in 2013. It is focussed on the classification of plant communities poorly investigated from a geobotanical standpoint. Altogether 125 phytosociological relevés were collected, using the Braun-Blanquet method. We noted more than 100 species in the vegetation plots, including many rare ones. We classified the vegetation into seven communities, including six associations. Five associations are proposed as new: Nymphaeetum nouchali, Fimbristylido miliaceae-Sphenocleetum zeylanicae, Ischaemo rugosi-Cyperetum pulcherrimi, Pentapeto phoeniceae-Aeschynomenetum indicae and Marsileetum minutae.…
Fonti, suggestioni e intersezioni classiche, tardoantiche e medievali nell’«Alda» di Guglielmo di Blois
2019
In questo saggio vengono studiate le fonti classiche, tardoantiche e medievali dell’«Alda», commedia elegiaca latina di Guglielmo di Blois (scritta verso il 1169-1170). In particolare, dopo una presentazione generale dell’opera e una rassegna critica degli studi e delle edizioni, ci si sofferma sulle suggestioni attinte a Terenzio, Virgilio, Orazio, Ovidio, Stazio, all’«Aegritudo Perdicae», Massimiano, Boezio, Ildeberto di Lavardin, ad alcune commedie elegiache del sec. XII (il «Geta» e l’«Aulularia» di Vitale di Blois, il «Pamphilus», la «Lidia» di Arnolfo d’Orléans) e alla tradizione novellistica “orientale” («Mischle Sendebar»). This essay is founded on the classical, late and medieval s…
Maturation of IgG avidity to individual rubella virus structural proteins.
2001
Background: the structural proteins of rubella virus, the capsid protein C and the envelope glycoproteins E1 and E2 were produced in lepidopteran insect cells using baculovirus expression vectors. The C-terminal ends of the corresponding proteins were fused to a polyhistidine tag for easy and gentle purification by metal ion affinity chromatography. Objectives: to investigate the maturation of natural and vaccinal IgG avidity against individual authentic and recombinant rubella virus (RV) structural proteins. Study design the analysis was carried out using a modified immunoblotting technique where the purified baculovirus-expressed proteins were compared with authentic rubella virus protein…
Targeting p53, hdm2, and CD19: vaccination and immunologic strategies.
2000
Peptides presented by class I major histocompatibility complex (MHC) molecules and derived from normal self-proteins that are expressed at elevated levels by cells from a variety of human (Hu) malignancies provide, in theory, potential target antigens for a broad-spectrum, cytotoxic T lymphocyte (CTL)-based immunotherapy of cancer and hematologic malignancies. However, as such tumor- and leukemia-associated self-proteins are also expressed at low levels in some types of normal tissues, such as thymus, spleen and lymphohemopoietic cells, these self-MHC-self-peptide complexes may also represent thymic and/or peripheral tolerogens, thereby preventing immune responses. This is particularly true…