Search results for "vidi"

Functionalized carbon nanotube for next generation biosensor

2013

Oligosaccharide recognition by selectins: Synthesis and biological activity of multivalent sialyl lewis-X ligands

1995

Abstract Trivalent sialyl Lewis-X ligands 6–8 anchored onto flexible templates have been synthesized and evaluated as inhibitors of E-selectin and P-selectin mediated cell adhesion in cell culture assays and in vivo. Biological activities in vitro correlated with spacer length and lead to ligands with 3-fold (E-selectin) and 5-fold (P-selectin) improved receptor binding avidity per single tetrasaccharide moiety.

Molecular Design of the Cαβ Interface Favors Specific Pairing of Introduced TCRαβ in Human T Cells

2008

Abstract A promising approach to adoptive transfer therapy of tumors is to reprogram autologous T lymphocytes by TCR gene transfer of defined Ag specificity. An obstacle, however, is the undesired pairing of introduced TCRα- and TCRβ-chains with the endogenous TCR chains. These events vary depending on the individual endogenous TCR and they not only may reduce the levels of cell surface-introduced TCR but also may generate hybrid TCR with unknown Ag specificities. We show that such hybrid heterodimers can be generated even by the pairing of human and mouse TCRα- and TCRβ-chains. To overcome this hurdle, we have identified a pair of amino acid residues in the crystal structure of a TCR that …

Layer-by-layer deposited multilayer assemblies of polyelectrolytes and proteins: from ultrathin films to protein arrays

2008

We have recently introduced a new method of creating ultrathin films of polyelectrolytes based on the electrostatic attraction between opposite charges. Multilayer assemblies are adsorbed in a layer-by-layer fashion from aqueous solutions of the polymers. The total film thickness can easily be adjusted by varying the ionic strength of the solution. Here, we report on the temperature stability and the water content of the multilayer assemblies. Furthermore, we have extended our concept to the incorporation of protein layers into films of synthetic polyelectrolytes. The well established system biotin/streptavidin was used to construct such multilayers, also by biospecific recognition. Adsorpt…

The avidin-theophylline complex: A structural and computational study

2023

: The interaction between avidin and its counterpart biotin is one of central importance in biology and has been reproposed and studied at length. However, the binding pocket of avidin is prone to promiscuous binding, able to accommodate even non-biotinylated ligands. Comprehending the factors that distinguish the extremely strong interaction with biotin to other ligands is an important step to fully picture the thermodynamics of these low-affinity complexes. Here, we present the complex between chicken white egg avidin and theophylline (TEP), the xanthine derivative used in the therapy of asthma. In the crystal structure, TEP lies in the biotin-binding pocket with the same orientation and …

AS “Rietumu bankas” finanšu analīze

2018

Autore darba ietvaros ir veikusi AS “Rietumu bankas” finanšu analīzi, novērtējot komercbankas gada pārskatus par 2013.–2017. gadu un veiktā analīze sniedz priekšstatu par esošo komercbankas darbību un aspektiem, kuri ir jāuzlabo nākamo pārskata gadu laikā. Darba mērķis: veikt komercbankas AS “Rietumu banka” finanšu analīzi un izstrādāt priekšlikumus darbības uzlabošanai. Darba uzdevumi: 1. Analizēt teorētiskos materiālus par komercbanku finanšu analīzi. 2. Raksturot AS “Rietumu bankas” darbību. 3. Analizēt AS “Rietumu bankas” gada pārskatus, izmantojot strukturālo un dinamisko analīzi. 4. Analizēt AS “Rietumu bankas” darbību raksturojošos rādītājus. 5. Izstrādāt priekšlikumus AS “Rietumu ba…

L’in house providing tra diritto europeo e diritto nazionale

2012

Nanodevices by DNA based gold nanostructures

2017

In this thesis DNA based structures were utilized to create gold nanostructures for nanosensing and nanoelectronic applications. In the past, both of these fields have been dominated by the conventional lithography methods, e.g., electron beam lithography and UV-lithography, but more recently scaling down the components by these techniques has become increasingly more complex and costly. Especially in the micro- and nanoelectronics, the increase in the component density and thus computational power would require fabrication of sub-10-nm components, which is challenging for the top-down approaches. Aforementioned developments have led researchers to seek alternative methods to fabricate these…

Naturālisma iezīmes Ovidija darbā Metamorfozes

2015

Bakalaura darba tēma ir Naturālisma iezīmes Ovidija darbā Metamorfozes. Bakalaura darba mērķis ir izpētīt, kāda nozīme ir naturālismam Ovidija poēmas Metamorfozes mākslinieciskajā veidojumā. Darbā tiek analizētas konkrētas epizodes, kurās jaušamas naturālisma iezīmes un tā funkcijas poēmā kopumā. Teorētiskā daļa, kas sastāv no divām nodaļām, tiek balstīta uz pieejamo zinātnisko un teorētisko literatūru, ar kuras palīdzību tiek noskaidrots naturālisma jēdziens un tam raksturīgās iezīmes. Praktiskā daļa tiek iedalīta divās galvenajās nodaļās un divās apakšnodaļās, kurās tiek pētīta naturālisma funkcija poēmā. Tiek secināts, ka ar naturālistiskiem aprakstiem, Ovidijs pauž savu viedokli par pas…

La fides e la promessa: forme di reciprocità tra dèi e uomini nella riscrittura di Ovidio

2017

Nelle rielaborazioni ovidiane dei racconti mitici greci, emergono diversi casi in cui la relazione tra esseri umani e divinità è riscritta alla luce della nozione romana di fides. Ma se la quasi totalità di questi miti, in cui il dio promette all’interagente mortale un pegno, un dono o un privilegio, è giustificata dalla presenza di vincoli di tipo amoroso, parentale o devozionale, un caso a parte rappresenta il racconto di Numa Pompilio e Giove (Fast. 3. 285-392) – un racconto Romano – in cui l’agente umano merita i pignora certa del dio e instaura con lui un rapporto di reciprocità. Il contributo si propone di esaminare questa rappresentazione della fides pollicita attraverso un confronto…