Search results for "wild"

Functional characterization of protein variants of the human multidrug transporter ABCC2 by a novel targeted expression system in fibrosarcoma cells

2012

The multidrug resistance-associated protein 2 (MRP2/ABCC2) is involved in the efflux of endogenous and xenobiotic substrates, including several anticancer and antiviral drugs. The functional consequences of ABCC2 protein variants remain inconsistent, which may be due to shortcomings of the in vitro assays used. To study systematically the functional consequences of nonsynonymous ABCC2 variants, we used a novel “Screen and Insert” (ScIn) technology to achieve stable and highly reproducible expression of 13 ABCC2 variants in HT1080 cells. Western blotting revealed lower (30–65%) ABCC2 expression for D333G, R1174H, and R1181L as compared with wild type (WT; 100%), whereas the linked variant V1…

Quantum algorithms for search with wildcards and combinatorial group testing

2012

We consider two combinatorial problems. The first we call "search with wildcards": given an unknown n-bit string x, and the ability to check whether any subset of the bits of x is equal to a provided query string, the goal is to output x. We give a nearly optimal O(sqrt(n) log n) quantum query algorithm for search with wildcards, beating the classical lower bound of Omega(n) queries. Rather than using amplitude amplification or a quantum walk, our algorithm is ultimately based on the solution to a state discrimination problem. The second problem we consider is combinatorial group testing, which is the task of identifying a subset of at most k special items out of a set of n items, given the…

NUTRITIONAL VALUE AND HEALTH PROMOTING EFFECTS OF WILD EDIBLE MUSHROOMS

2013

Gli OGM e la flora italiana

2007

IL RUOLO DELL’OLEASTRO INVEGES DI SCIACCA NELLA DEFINIZIONE DEL PAESAGGIO FORESTALE STORICO DELLA SICILIA

2016

L’oleastro, Olea europaea var. sylvestris (Mill.) Lehr, è uno delle componenti rappresentative della vegetazione mediterranea. Specie considerata caratteristica della macchia (Oleo-Ceratonion siliquae Br.-Bl. ex Guinoch. e Drouin. 1944) si accompagna con altre sclerofille e specie caducifoglie estive con le quali forma aspetti di vegetazione alto arbustiva o arborescente. Ma l’oleastro in Sicilia è anche un elemento di boschi termofili, di territori che presentano una certa xericità ambientale, potenzialmente diffusi in un’ampia fascia altimetrica che si estende dalla costa fino a 800-1000 m di quota. In tale contesto, l’oleastro si accompagna con querce caducifoglie termofile, quali Quercu…

The role of second and third line tyrosine kinase inhibitor monotherapy in EGFR wild-type (and unknown mutational status) advanced non-small-cell lun…

2015

I am not English. I am Irish which is quite another thing: Reconsidering Wilde’s Irishness

2023

Even though O. Wilde's Irishness is difficult to trace, there is a profound sense of Irishness underlying his life and much of what he wrote and thought, especially in his correspondence. In my paper, I evaluate the role of Irishness within his life and work in order to assess how it shapes and influences his own thinking and writing. Perhaps considered as one facet of his all-interesting multifarious doubleness, still, it occupies a specific role within reception studies and explains why there has always been a strong interest towards his life and work among his fellow countrymen.

C172S Substitution in the Chloroplast-encoded Large Subunit Affects Stability and Stress-induced Turnover of Ribulose-1,5-bisphosphate Carboxylase/Ox…

1999

Previous work has indicated that the turnover of chloroplast ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco; EC 4.1.1. 39) may be controlled by the redox state of certain cysteine residues. To test this hypothesis, directed mutagenesis and chloroplast transformation were employed to create a C172S substitution in the Rubisco large subunit of the green alga Chlamydomonas reinhardtii. The C172S mutant strain was not substantially different from the wild type with respect to growth rate, and the purified mutant enzyme had a normal circular dichroism spectrum. However, the mutant enzyme was inactivated faster than the wild-type enzyme at 40 and 50 degrees C. In contrast, C172S mutant …

Cysteines 449 and 459 modulate the reduction-oxidation conformational changes of ribulose 1.5-bisphosphate carboxylase/oxygenase and the translocatio…

2006

The role of cysteines 449 (Cys449) and 459 (Cys459) from the large subunit (LS) of ribulose 1-5-bisphosphate carboxylase/oxygenase (Rubisco) in the reduction-oxidation (redox) regulation of the enzyme was assessed by site-directed mutagenesis of these residues and chloroplast transformation of Chlamydomonas reinhardtii. In vitro studies indicated that mutations C449S, C459S or C449S/ C459S do not affect the activity and proteolytic susceptibility of the enzyme in the reduced state. However, when oxidized, the mutant enzymes differed from the wild type (WT), showing an increased resistance to inactivation and, in the case of the double mutant (DM), an altered structural conformation as refle…

Identification, ecology and use blood meals from hematophagous Diptera (Glossinidae, Stomoxys and Tabanidae) for noninvasive sampling of wildlife in …

2015

The contact between human and wild fauna has considerably increased during these last decades due to the increase of human population size but also to conservation policies. As a consequence, the number of zoonotic diseases soared with a mean of six new infectious diseases per year, 75% of whom being vectorially transmitted. The way to avoid the human contamination by these emergent diseases is based on the efficient vector control resulting from a deep knowledge of the ecology and the feeding behavior of the different vector species. During our work, we have identified and characterized the ecology of 6 tsetse species (Glossina palpalis palpalis, G. fuscipes fuscipes, G. fusca congolense, …