Search results for "β-subunit"

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Sugar moiety has a direct influence on the secondary structure and properties of sturgeon gonadotropin

2009

Sugar moiety has a direct influence on the secondary structure and properties of sturgeon gonadotropin Sturgeon Acipenser güldenstädti Br. gonadotropic hormone (GTH) β-subunit (β-GTH) was selectively chemically deglycosylated (dg) by anhydrous hydrogen fluoride and trifluoromethane sulfonic acid. Two dgβ-GTH molecular forms retaining 35% (dgβ-GTH1) and 13% (dgβ-GTH2) of their initial carbohydrates were obtained. Investigation of the reassociated α-β hybrid dimers (recombinants) α-GTH+dgβ-GTH1 and α-GTH+dgβ-GTH2 showed that the immunoreactivity with antiserum raised against standard GTH dropped by 22.5%. Hybrid dimers were recognised by the standard GTH antibodies, which indicated that the s…

endocrine systemMultidisciplinaryGeneral interestmedicine.drug_classChemistrySciencesecondary structure of the α-βQdeglycosylationdimersturgeon gonadotropic hormoneSturgeonBiochemistrymedicineSugar moietyβ-subunitGonadotropinProtein secondary structurebiological functionProceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences.
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