0000000000001688
AUTHOR
Vincent Grandperret
Les histones désacétylases de type 2 (HD2) : caractérisation fonctionnelle dans l'immunité des plantes
Les histones désacétylases (HDAC) sont des enzymes qui catalysent les réactions de désacétylation des protéines, une modification post-traductionnelle caractérisée par le retrait d’un groupement acétyle des chaînes latérales des résidus de lysine des protéines. Chez les plantes, il existe trois familles de HDAC. Les deux premières – les familles RDP3/HDA1 et SIR2 – sont communes à tous les eucaryotes, alors que la troisième – la famille des HD2 – est spécifique des plantes. Chez le tabac, des HD2 ont été identifiées comme des régulateurs négatifs de la réponse hypersensible induite par la cryptogéine, un éliciteur protéique. La réponse hypersensible est une réponse de défense caractérisée p…
Etude de la régulation d'une histone déacetylase de type 2 dans le contexte des réactions de défense des plantes
Rapport de stage de Master 2, Spécialité Biochimie Biologie Cellulaire et Moléculaire SPE IPM CT non renseigné car non soutenu par INRA; Master
Type-II histone deacetylases: elusive plant nuclear signal transducers
Since the beginning of the 21st century, numerous studies have concluded that the plant cell nucleus is one of the cellular compartments that define the specificity of the cellular response to an external stimulus or to a specific developmental stage. To that purpose, the nucleus contains all the enzymatic machinery required to carry out a wide variety of nuclear protein post-translational modifications (PTMs), which play an important role in signal transduction pathways leading to the modulation of specific sets of genes. PTMs include protein (de)acetylation which is controlled by the antagonistic activities of histone acetyltransferases (HATs) and histone deacetylases (HDACs). Regarding p…
NO signaling in cryptogein-induced immune responses in tobacco
Evolutionary diversification of type-2 HDAC structure, function and regulation in Nicotiana tabacum
Ministère de l'Education Nationale et de la Recherche ; Conseil Régional de Bourgogne (PARI AGRALE8) ; Association pour la Recherche sur les Nicotianacées ; Conseil Régional de Bourgogne; International audience; Type-2 HDACs (HD2s) are plant-specific histone deacetylases that play diverse roles during development and in responses to biotic and abiotic stresses. In this study we characterized the six tobacco genes encoding HD2s that mainly differ by the presence or the absence of a typical zinc finger in their C-terminal part. Of particular interest, these HD2 genes exhibit a highly conserved intron/exon structure. We then further investigated the phylogenetic relationships among the HD2 gen…
Les histones déacétylases de type 2 (HD2): des régulateurs importants de l'immunité innée chez le tabac
Interplays between nitric oxide and reactive oxygen species in cryptogein signalling
The cellular messenger nitric oxide (NO) has many functions in plants. In this study, we investigated its interplays with Reactive Oxygen Species (ROS) in the defense responses triggered by the elicitin cryptogein produced by the oomycete Phytophthora cryptogea. The production of NO induced by cryptogein in tobacco cell suspensions was partly regulated through a ROS-dependent pathway involving the NADPH oxidase NtRBOHD. In turn, NO down-regulated the level of H2O2 derived from NtRBOHD activity. Both NO and ROS synthesis appeared to be under the control of two redundant isoforms of histone deacetylases of type 2 acting as negative regulators of cell death. Occurrence of an interplay between …