Expression and localization of MMP-9, MMP-2 and iNOS in some sample of human dental pulp orthodonticaly treated.

Acute intermittent nicotine treatment enhances proliferation of neuronal precursor cells in the subventricular zone (SVZ) of adult rat brain

Role of Matrix Metalloproteinases 2,9 (MMP-2,-9), Orphanin FQ and Others Peptides in Vascular Tone of Human Umbilical Cord at Term

Umbilical cord is the structure that connects the fetus to the placenta. Umbilical cord at term is made of three vessels surrounded by the Wharton's Jelly, a mocous connective tissue rich in glycosaminoglycans, proteoglycans and different types of collagens and other microfibrils, which are responsible for its elasticity and its mechanical properties. Umbilical cord seems to lack any capillaries or lymphatics and umbilical vessels are unique in lacking any innervation of the vascular tone, and the expression of some enzymes responsible for the remodelling of the extracellular matrix. Trough immunihistochemistry we investigated the expression of matrix metallo-peptidase 2 and 9 (MMP-2, MMP-9…

Immunohistochemical expression and apoptotic role of Caspase9 in aortic aneurysms

Immunohistochemical Localization of Epithelial, Endothelial and Muscular Cell Differentiation Markers in Human Umbilical Cord

Introduction. Since time [1], our group studies through immunohistochemistry and RT-PCR analysis the expression of many genes and the localization of the corresponding proteins in the human umbilical cord [2], with the aim to create a map of the presence of the different molecules in the different territories of this organ. In this work we investigate the expression and localization of several cell differentiation markers in the different territories of the human umbilical cord. Materials and methods. The presence of E-Cadherin, CD31, CD68, AE1-AE3, Desmin, Actin ML, HHF35 and MIB-1 was studied through immunohistochemistry. Results. The expression of E-Cadherin (epithelial cell marker) has …

Ambiente staminale e prospettive di transdifferenziamento verso il fenotipo osteoblastico

Bovine seminal vesicles ANP, Oxytocin, immunohistochemical expression.

Expression of β-Endorphin and Caspase-9 in Orthodontically Treated Dental Pulp.

Introduction. For several years, our histology laboratory has worked in co-operation with the Winchmore Hill Dental Practice studying the activity of Acetylcholinesterase and some neuropeptides (CGRP, Sub. P, Enkephalins, Endorphins)(1) in orthodontic treated human dental pulp to clarify force's action. It seems that recent data do not yet, fully elucidate the exact effects of the force applied on teeth. For this reason we are investigating whether the traction is involved in some cases of pulpal death. In this study we focused on the expression of Caspase-9 and β-Endorphine. Materials and Methods. The expression of the 2 peptides has been identified through immunohistochemistry assay: not …

Role of matrix metalloproteases 2, 9 (MMP-2, -9), orphanin FQ and others peptides in vascular tone of human umbilical cord at term

Umbilical cord is the structure that connects the fetus to the placenta. Umbilical cord at term is made of three vessels surrounded by the Wharton’s jelly, a mucous connective tissue rich in glycosaminoglycans, proteoglycans and different types of collagens and other microfibrils, which are responsible for its elasticity and its mechanical properties.Umbilical cord seems to lack any capillaries or lymphatics and umbilical vessels are unique in lacking any innervation; thus endothelial cells may play the major role in the local control of the blood flow. Objectives. In this paper we investigated in human umbilical cord at term the expression of some peptides involved in the regulation of the…

METABOTROPHIC GLUTAMATE RECEPTORS (MGLUR1/5 AND MGLUR2/3) ACTIVATION BY AGONISTS TREATMENT INCREASES FGF-2 AND BDNF IN RAT AND MOUSE BRAIN AND INDUCES NEUROPROTECTION

Expression and localization of matrix metalloproteinases MMPs in human umbilical cord

Regulation of neurogenesis in the adult brain

Molecular mechanism of FGF-2 gene regulation by nicotinic receptors

Imaging ecografico del cordone ombelicale umano.

Human Umbilical Cord at Term. Immunohistochemical expression of MMP-2, MMP-9, Orphanin F/Q and others Peptides involved in the regulation of vascular tone

B-Endorphin in Human Dental Pulp During Orthodontic Treatments

Imaging ecografico del cordone ombelicale umano Ecographic imaging of the human umbilical cord

Immunohistochemical and transcriptional expression of the matrix metalloproteinases MMP-2 and MMP-9 in normal and pathological human oral mucosa

The oral cavity is exposed to chronic or recurrent, physical and chemical trauma that could lead to mucosal reactions (e.g. hyperplasia, dysplasia and tumors). The objective of this study is to investigate the expression and the possible changes of the two matrix metalloproteinases MMP-2 and MMP-9 in normal and pathological human oral mucosa samples. Normal oral mucosa samples and three different types of pathological conditions (hyperplasia, dysplasia and carcinoma) were used for this study. Immunohistochemical analysis was used to evaluate protein expression for the two enzymes, while Reverse Transcription ? Polymerase Chain Reaction (RT-PCR) was used to investigate gene expression. Image…

ACUTE INTERMITTENT NICOTINE TREATMENT ENHANCES PROLIFERATION OF NEURONAL PRECURSOR CELLS IN THE SUBVENTRICOLAR ZONE (SVZ) OF ADULT AND AGED RAT BRAIN

Expression of remodelling enzymes (MMP2-MMP9) in samples of salivary gland's tumors

Immunohistochemical localization of MMP-9 and Orphanin in some sample of dental pulp.

Expression of cytokeratins in human umbilical cord at term

Background. Cytokeratins are the proteins that costitute intermediate filaments. In the cytoplasm, keratin filaments extend from the nucleus to the plasma membrane. This link is important for the structural organization of the cytoplasm and for many cellular processes such as mitosis, cell movement, cell differentiation and adhesion. The pattern of expression of cytokeratins is frequently organ or tissue specific and depends mainly on the type of epithelium, the level of differentiation and the stage of development. The expression of cytokeratins in umbilical cord has been investigated by many groups, but these previous studies gave only fragmentary informations: each single study focused o…

Expression of remodelling enzymes (MMP-2, MMP-8, MMP-9) in mixed tumors of salivary gland

Twenty years' history of immunohistochemical mammals dental pulps studies.

Functional Aspects of Adrenocholinergic and Peptidergic Innervation about Prostate and Seminal Vesicles in Mammals: A Review

This study was aimed to explain the functional role of mediation in the organs of the male reproductive system (prostate and seminal vesicles of rat and hamster), representing throught the visualization of adrenergic fibres (Falck Method), cholinergic fobres (Martinez-Rodriguez method) and by immunohistochemical methods to detect the P substance and the CGRP (PAP) in glandular structures. The adrenocholinergic and peptidergic mediators have a growing interest for their synergic action in the control of contractility, vasodilatation and most likely, of the secretory activity of the epithelium, probably expressed by andocrine-paracrine mechanisms.

Expression of Remodelling Enzymes (MMP-2, MMP-9) in Normal and Pathological Oral Mucosa Samples

Introduction. The oral cavity contains many structures compressed in a small area. It is exposed to chronic or recurrent, physical and chemical trauma leading to mucosal reactions (e.g. hyperplasia, dysplasia and tumors). For a long time, our group has been studying the expression of some metalloproteinases (MMP-2, MMP-9) in healthy and pathological human oral mucosa through immunohistochemistry and RT-PCR to investigate the entity of the remodelling process in the different cases. Matherials and Methods. In this paper we investigate a sample of normal oral mucosa and four pathological samples (mucocele, fibroma, dysplasia and carcinoma). We identified the localization of MMP-2 and MMP-9 th…

Expression of Remodelling Enzymes MMPs in Hernia Formation

Cell differentiation markers in human umbilical cord; an immunohistochemical study

The full-term human umbilical cord is made of three blood vessels (two arteries and a vein) surrounded by the Wharton’s jelly, a soft connective tissue made of few mesenchymal stromal cells and an abundant extracellular matrix. Both endothelial and Wharton’s jelly cells are versatile in their differentiation potential; they have shown to be able to differentiate into several cell lineages. Since the differentiation potential of these cells is very wide, we thought it could be interesting to investigate the expression of several cell differentiation markers in the different regions of human umbilical cord. Immunohistochemistry showed the expression of E-cadherin and cytokeratins in the amnio…

Vent'anni di storia sullo studio immunoistochimico delle polpe dentali di mammiferi Twenty years'history of immunohistochemical mammals dental pulp studies

Cytocheratins Identification in Human Umbilical Cord at Term.

Introduction. Cytokeratins (Cks) are the proteins that constitute intermediate filaments. The pattern of expression of cytokeratins is frequently organ or tissue specific and depends mainly on the type of epithelium, the level of differentiation and the stage of development. With this study we tried to make light on the pattern of expression of a wide range of CKs in human umbilical cord at term. Materials and methods. We investigated the expression of CKs through immunohistochemistry and RT-PCR assay. Results. AE1/AE3 antibody reacts with the basic CKs 1, 2, 3, 4, 5, 6, 7 and 8, and with the acidic CKs 10, 13, 14, 15, 16 and 19. The reactivity for this antibody is localized in the amniotic…

Expression of matrix metalloproteinases in full-term human umbilical cord and Human Umbilical Vein Endothelial Cells.

Background. Matrix metalloproteinases (MMPs) are extracellular zinc-dependent endopeptidases involved in breakdown and remodelling of extracellular matrix in physiological and pathological processes. MMPs have also a role on cell proliferation, migration, differentiation, angiogenesis and apoptosis. Umbilical cord is a special organ subjected to many changes during pre-natal life and whose cells can maintain a certain degree of plasticity also in post-natal period; for example recently they have been used as a source of stem cells. For these reasons we believe it is interesting to investigate the expression of some remodelling enzymes in this tissue. Human Umbilical Vein Endothelial cells (…

Acute intermittent nicotine treatment counteracts the reduced proliferation of neuronal precursor cells of the subventricular zone (svz) in aged rat brain