0000000000006565

AUTHOR

Martin A. Cheever

showing 3 related works from this author

Dendritic Cells Lose Ability to Present Protein Antigen after Stimulating Antigen-Specific T Cell Responses, despite Upregulation of MHC Class II Exp…

2000

Abstract Immature dendritic cells (DC) take up, process and present protein antigens; mature DC are specialized for stimulating primary T cell responses with increased expression of MHC class II and co-stimulatory molecules, but are incapable of processing and presenting soluble protein. The current study examined whether maturation of DC is triggered by T cell recognition of antigens presented by immature DC. Human DC derived from CD34+ progenitor cells by culture with granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-6 (IL-6) in serum-free medium could prime naive CD4+ T cells to keyhole limpet hemocyanin (KLH) and ovalbumin (OVA). The cultured DC retained the abil…

CD4-Positive T-LymphocytesTime FactorsOvalbuminT cellImmunologyCD1Bone Marrow CellsCell CommunicationCulture Media Serum-FreeInterferon-gammaInterleukin 21medicineHumansImmunology and AllergyCytotoxic T cellIL-2 receptorCD40 AntigensAntigen-presenting cellCells CulturedAntigen PresentationMHC class IIbiologyInterleukin-6Tumor Necrosis Factor-alphaHistocompatibility Antigens Class IIGranulocyte-Macrophage Colony-Stimulating FactorCell DifferentiationDendritic CellsHematologyIntercellular Adhesion Molecule-1Natural killer T cellMolecular biologyCoculture Techniquesmedicine.anatomical_structureHemocyaninsB7-1 Antigenbiology.proteinImmunobiology
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In Vitro Priming to Tumor-Associated Proteins

1997

Cancer can be cured in mice by adoptive transfer of T cells specific for the malignant cells or by vaccination to tumor-specific antigens. The application of immunotherapy to the treatment of human cancer hinges on the identification of human tumor antigens to which specific immunity can be elicited.

Adoptive cell transferbiologybusiness.industrymedicine.medical_treatmentfungifood and beveragesPriming (immunology)ImmunotherapyDendritic cellIn vitroVaccinationAntigenmedicineCancer researchbiology.proteinbusinessKeyhole limpet hemocyanin
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Rapid High Efficiency Sensitization of CD8+ T Cells to Tumor Antigens by Dendritic Cells Leads to Enhanced Functional Avidity and Direct Tumor Recogn…

2003

Abstract Myeloid-origin dendritic cells (DCs) can develop into IL-12-secreting DC1 or non-IL-12-secreting DC2 depending on signals received during maturation. Through rapid culture techniques that prepared either mature, CD83+ DC1 or DC2 from CD14+ monocytes in only 2 days followed by a single 6–7 day DC-T cell coculture, we sensitized normal donor CD8+ T cells to tumor Ags (HER-2/neu, MART-1, and gp100) such that peptide Ag-specific lymphocytes constituted up to 16% of the total CD8+ population. Both DC1 and DC2 could sensitize CD8+ T cells that recognized peptide-pulsed target cells. However, with DC2, a general decoupling was observed between recognition of peptide-pulsed T2 target cells…

AdultCytotoxicity ImmunologicMaleReceptor ErbB-2CD8 AntigensT cellImmunologyAntigen presentationEpitopes T-LymphocyteStreptamerCD8-Positive T-LymphocytesBiologyLymphocyte ActivationInterleukin 21MART-1 AntigenAdjuvants ImmunologicAntigens NeoplasmT-Lymphocyte SubsetsCell Line TumorCell AdhesionmedicineHumansImmunology and AllergyCytotoxic T cellIL-2 receptorAntigen-presenting cellMelanomaCells CulturedAntigen PresentationMembrane GlycoproteinsCell DifferentiationDendritic CellsInterleukin-12Peptide FragmentsNeoplasm ProteinsCell biologymedicine.anatomical_structureCulture Media ConditionedImmunologyInterleukin 12FemaleImmunizationgp100 Melanoma AntigenThe Journal of Immunology
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