0000000000009105

AUTHOR

Ursula Antes

showing 3 related works from this author

Evidence for the presence of autoantibodies to the collagen-like portion of C1q in systemic lupus erythematosus.

1988

We investigated the connection between the C1q solid-phase binding assay (C1q SPBA) and double-stranded DNA antibodies, and analyzed the immune complex material in systemic lupus erythematosus (SLE) sera. Comparison with a new monoclonal assay for C1q-bearing immune complexes (the 242G3 assay) revealed that the immune complexes in SLE bind specifically to solid-phase C1q, and not to fluid-phase C1q. The C1q solid-phase binding activity sedimented as 7S IgG, was insensitive to DNase treatment, and could be selectively absorbed by C1q-coupled beads and by bovine serum albumin-anti-bovine serum albumin C1q beads, but not by DNA. Thus, antibodies to double-stranded DNA do not interfere in the C…

Complement Activating EnzymesImmunologySerum albuminchemical and pharmacologic phenomenaEnzyme-Linked Immunosorbent AssayAntigen-Antibody Complexurologic and male genital diseasesfluids and secretionsImmune systemRheumatologyimmune system diseasesComplement C1medicineImmunology and AllergyHumansLupus Erythematosus SystemicPharmacology (medical)Bovine serum albuminskin and connective tissue diseasesAutoantibodiesLupus erythematosusbiologybusiness.industryLigand binding assayComplement C1qAutoantibodyDNA Neoplasmmedicine.diseaseImmune complexImmunoglobulin GImmunologybiology.proteinCollagenAntibodybusinessUltracentrifugationArthritis and rheumatism
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C1q-bearing immune complexes detected by a monoclonal antibody to human C1q in rheumatoid arthritis sera and synovial fluids

1991

Using a monoclonal antibody directed against the C-chain of human C1q, we detected C1q-bearing immune complexes (IC) in sera and synovial fluids of rheumatoid arthritis (RA) patients. In a sandwich-ELISA, C1q-bearing IC were captured by the solid-phase monoclonal antibody and then detected with peroxidase-labeled F(ab')2-antibodies to either human IgG or IgM. The results of this assay were compared to an ELISA-modification of the C1q-solid-phase binding assay (C1q-SPBA). C1q-bearing IC were detected in 81.1% of RA-sera and the 65.2% of RA-synovial fluids. IgG as well as IgM was present in 72.6% of the sera and 70% of the synovial fluids which were positive in both assays. Most RA sera that …

medicine.drug_classImmunologyEnzyme-Linked Immunosorbent Assaychemical and pharmacologic phenomenaAntigen-Antibody ComplexMonoclonal antibodyComplement Hemolytic Activity AssayArthritis RheumatoidImmunoglobulin Fab FragmentsClassical complement pathwayImmune systemRheumatologyimmune system diseasesOsteoarthritisSynovial FluidmedicineHumansImmunology and AllergySynovial fluidskin and connective tissue diseasesbiologybusiness.industryComplement C1qAntibodies Monoclonalmedicine.diseaseImmune complexImmunoglobulin MImmunoglobulin GRheumatoid arthritisMonoclonalImmunologybiology.proteinAntibodybusinessRheumatology International
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Autoantikörper gegen die Komplementkomponente C1q beim Systemischen Lupus Erythematodes

1990

Autoantibodies against C1q, a subcomponent of the first complement component C1, could be detected in 49.4% of sera from patients with systemic lupus erythematosus (SLE). They are directed against the collagen-like portion of the C1q molecule and recognize only bound, but not fluid-phase C1q. The appearance of these autoantibodies in the course of SLE correlates with the detection of IgG in the C1q-Solid-Phase-Bindingassay, with high titres of dsDNA-antibodies and with depressed total complement activity (CH50) and C1q-values. Our investigations show that autoantibodies against the collagen-like portion of bound C1q but not immune complexes are the main constituent of C1q-binding IgG in SLE.

business.industryAutoantibodychemical and pharmacologic phenomenaGeneral Medicineurologic and male genital diseasesComplement activityfluids and secretionsImmune systemimmune system diseasesDrug DiscoveryImmunologyMolecular MedicineMedicineskin and connective tissue diseasesbusinessGenetics (clinical)Anti-SSA/Ro autoantibodiesKlinische Wochenschrift
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