Additional file 1 of Experimental community coalescence sheds light on microbial interactions in soil and restores impaired functions

Additional file 1: Supplementary Table 1. Number of OTUs significantly differentially abundant among all treatments as estimated using a generalized linear mixed model, among the 515 most abundant 16S rRNA OTUs and the 439 most abundant 18S rRNA OTUs. Supplementary Figure 1. Abundances of total bacteria and total fungi. Quantification of 16S rRNA (a and c) and ITS (b and d) gene copy numbers in the original soil, the removal treatments and the control (Step 1; a and b) and in the coalescence treatment, the self-mixed removal treatment and the control samples (Step 2; c and d) (mean ± s.e. of log10-transformed data expressed as gene copy g-1 dry soil). Letters indicate significantly differen…

Deciphering biotic interactions and their role in soil microbial community assembly

National audience; Soil microbial communities play key roles in ecosystem functioning. Yet, little is known aboutthe importance of microbe-microbe interactions in soil microbial community assembly andfunctions. To address this knowledge gap, the objective of my thesis is to assess the role ofinteractions between micro-organisms in soil microbial communities. For this purpose, we willmanipulate the interactions between microorganisms within complex soil microbialcommunities by (i) removing different members of the soil community, (ii) adding microbialtaxa to the soil community and (iii) increasing the physical distance between members of thesoil community. How shifts in microbial interaction…

Unusual presentation of blastic plasmacytoid dendritic cell neoplasm: Pitfalls in other hematolymphoid neoplasms

Abstract Blastic plasmacytoid dendritic cell neoplasm (BPDCN) is a rare CD4+/CD56+ hematological malignancy with aggressive clinical course and poor prognosis. Histologically, BPDCN is characterized by a diffuse, monomorphous infiltration of cutaneous, subcutaneous, and sometimes other tissues such as lymph nodes and bone marrow, by medium-sized neoplastic cells with blastoid morphology. Typically, there is absence of lymphocytic infiltrate. Diagnosis relies on immunophenotypic expression of CD4, CD56, and the more specific markers of plasmacytoid dendritic cells CD123, CD303/BDCA2, and TCL1. We report a case of a 57-year-old man who presented a 4 cm-long solitary, erythemateous lesion on t…

Les communautés de bactéries des sols. De réseaux plantes / pollinisateurs.

Experimental community coalescence sheds light on microbial interactions in soil and restores impaired functions

Abstract Background Microbes typically live in communities where individuals can interact with each other in numerous ways. However, knowledge on the importance of these interactions is limited and derives mainly from studies using a limited number of species grown in coculture. Here, we manipulated soil microbial communities to assess the contribution of interactions between microorganisms for assembly of the soil microbiome. Results By combining experimental removal (taxa depletion in the community) and coalescence (mixing of manipulated and control communities) approaches, we demonstrated that interactions between microorganisms can play a key role in determining their fitness during soi…

Deciphering biotic interactions and their role in soil microbial community assembly and functions: using community manipulation to steer soil microbial communities

Microbial communities play a key role in ecosystem functioning as well as in plant, animal, and human welfare. Their assembly relies on different processes commonly referred to as abiotic and biotic filters. Despite the widespread emphasis on abiotic filters in terrestrial ecosystems, a growing body of evidence suggests that interactions between microorganisms play a critical role in community assembly. However, the contribution of these interactions to microbial community assembly and the factors influencing them have not been clearly established. In most cases, biotic interactions between microorganisms have been investigated based on cocultures of a handful of strains, completely overloo…

Deciphering Biotic Interactions and Their Role in Soil Microbial Community Assembly and Functions

Microbial communities play a key role in ecosystem functioning as well as in plant, animal, and human welfare. Their assembly relies on different processes commonly referred to as abiotic and biotic filters. Despite the widespread emphasis on abiotic filters in terrestrial ecosystems, a growing body of evidence suggests that interactions between microorganisms play a critical role in community assembly. However, the contribution of these interactions to microbial community assembly and the factors influencing them have not been clearly established. In most cases, biotic interactions between microorganisms have been investigated based on cocultures of a handful of strains, completely overloo…