How challenging RADseq data turned out to favor coalescent-based species tree inference. A case study in Aichryson (Crassulaceae)

Analysing multiple genomic regions while incorporating detection and qualification of discordance among regions has become standard for understanding phylogenetic relationships. In plants, which usually have comparatively large genomes, this is feasible by the combination of reduced-representation library (RRL) methods and high-throughput sequencing enabling the cost effective acquisition of genomic data for thousands of loci from hundreds of samples. One popular RRL method is RADseq. A major disadvantage of established RADseq approaches is the rather short fragment and sequencing range, leading to loci of little individual phylogenetic information. This issue hampers the application of coa…

A new combination in Mononeuria (Caryophyllaceae)

The nomenclatural change Mononeuria caroliniana comb. nov. is proposed on the basis of molecular phylogenetic results analyzing the nuclear internal transcribed spacer.

What is the origin of the Scottish populations of the European endemic Cherleria sedoides (Caryophyllaceae)?

Cherleria sedoides L. (Minuartia sedoides (L.) Hiern) is a montane perennial which, with some species in Minuartia sect. Spectabiles, is more closely related to Scleranthus than to other Minuartia species and is therefore best restored to the reinstated and redefined genus Cherleria. Reconstruction of the ancestral area of the clade containing C. sedoides suggests that it evolved in the Alps or the Balkan peninsula. The species now has an unusual distribution, being present in the mountains of southern Europe and Scotland but absent from the Arctic. Three historical scenarios that might have led to the presence of the species in Scotland are outlined and tested by a molecular analysis compa…

Simultaneous speciation in the European high mountain flowering plant genus Facchinia (Minuartia s.l., Caryophyllaceae) revealed by genotyping-by-sequencing

Understanding the relative importance of different mechanisms of speciation in a given lineage requires fully resolved interspecific relationships. Using Facchinia, a genus of seven species centred in the European Alps, we explore whether the polytomy found by Sanger sequencing analyses of standard nuclear (ITS) and plastid markers (trnQ-rps16) is a hard or soft polytomy by substantially increasing the amount of DNA sequence data, generated by genotyping-by-sequencing. In comparison to 142 phylogenetically informative sites in the Sanger sequences the GBS sequences yielded 3363 phylogenetically informative sites after exclusion of apparently oversaturated SNPs. Maximum parsimony, maximum li…

A conspectus of the genus Cherleria (Minuartia s.l., Caryophyllaceae)

Abstract: Minuartia s.l. (Caryophyllaceae) is polyphyletic, with its species belonging to eleven major clades, all of which have been recognized at the generic rank. Cherleria is one of these segregate genera, based on the Linnaean species Cherleria sedoides. Its centre of diversity is on the Balkan Peninsula, but species also occur in the European and North American high mountains and in the Arctic. The species of Cherleria show ecological, especially substrate, differentiation and multiple colonisations of alpine habitats. We make new combinations for the 17 (of 20) taxa in Cherleria that do not yet have Cherleria names and provide a key to all species of the genus. Citation: Moore A. J. …

Which changes are needed to render all genera of the German lora monophyletic?

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Two new combinations inAdenostyles (Asteraceae, Senecioneae), a conspectus of the genus and key to its species and subspecies

Abstract Dillenberger M. S. & Kadereit J. W.: Two new combinations in Adenostyles (Asteraceae, Senecioneae), a conspectus of the genus and key to its species and subspecies. — Willdenowia 42: 57-61. June 2012. — Online ISSN 1868-6397; © 2012 BGBM Berlin-Dahlem. Stable URL: http://dx.doi.org/10.3372/wi.42.42105 Based on a molecular phylogeny (using ITS, ETS and the rpl32-trnL, psbA-trnH and ndhF-rpl32 plastid spacer sequences) of all species and subspecies of the European genus Adenostyles currently recognised, and supported by the morphology of the leaf margin, we transfer two subspecies of A. alliariae to A. alpina and provide the new combinations A. alpina subsp. macrocephala and A. alpin…

A conspectus of Tephroseris (Asteraceae: Senecioneae) in Europe outside Russia and notes on the decline of the genus

Tephroseris is generally considered a difficult genus. Based on the examination of extensive herbarium material and considering the existing literature, we recognize seven species in Europe outside Russia. These are T. palustris, T. integrifolia with subsp. integrifolia, subsp. aurantiaca, subsp. capitata, subsp. maritima, subsp. serpentini and subsp. “tundricola”, T. balbisiana, T. crispa, T. helenitis, T. longifolia and T. papposa. Phylogenetic analysis of ITS and ETS sequences showed that these species fall into three lineages. These are: (1) T. palustris, clearly related to Arctic species of the genus; (2) T. integrifolia; and (3) the remaining species. Molecular dating of the T. integr…

Maximum polyphyly: Multiple origins and delimitation with plesiomorphic characters require a new circumscription of Minuartia (Caryophyllaceae)

The phylogeny of the European high mountain genus Adenostyles (Asteraceae-Senecioneae) reveals that edaphic shifts coincide with dispersal events.

UNLABELLED PREMISE OF THE STUDY Heterogeneity of edaphic conditions plays a large role in driving the diversification of many plant groups. In the Alps and other European high mountains, many closely related calcicole and calcifuge plant taxa exist. To better understand patterns and processes of edaphic differentiation, the phylogeny of the edaphically variable genus Adenostyles was studied. The genus contains three species, of which A. alpina has five subspecies. Each species and subspecies is largely confined to either calcareous or noncalcareous substrates. • METHODS We analyzed the phylogeny of Adenostyles using DNA sequences of nrITS, nrETS, nuclear chalcone synthase, and three plastid…